Navigation Links
pET GST Fusion System 41 from Novagen

ProductspET GST Fusion System 41 from Novagen
Company Novagen
Item pET GST Fusion System 41
Price $373.00
Description 

Schistosomal glutathione-S-transferase (GST) is commonly used as a fusion partner when expressing proteins in E. coli (1). The GSTTag sequence has been reported to enhance the production and in some cases the solubility of its fusion partners. When expressed in a soluble, properly folded form, GSTTag fusion proteins can be purified with immobilized glutathione. Gentle elution is achieved with buffers containing reduced glutathione. Quantification of soluble GST fusions is also possible by assaying the transferase activity. In order to further enhance the utility of GST as a fusion tag, Novagen has designed two new multi-frame pET vectors, pET-41a-c(+) and pET-42a-c (+). In addition to featuring the powerful T7lac promoter, these vectors encode the GSTTag (220 aa), proteolytic sites, HisTag (6 aa) and STag (15 aa) sequences.

In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the generation of native proteins with their authentic N-terminal residues.

The HisTag and STag sequences enable alternative protein detection and purification procedures to be performed; for example, when enhancing purity with a separate purification method, or when purifying under denaturing conditions.

1. Smith, D.B. and Johnson, K.S. (1988) Gene 67, 3140.

Info NovagenNovagen
441 Charmany Drive
Madison, WI 53719
Customer Service: 800-526-7319
Tech Support: 800-207-0144
Fax Number: 608-238-1388
Web Site: http://www.novagen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. pET GST Fusion System 42 plus Competent Cells from Novagen
2. pET GST Fusion System 42 from Novagen
3. pET GST Fusion System 41 plus Competent Cells from Novagen
4. His-Patch ThioFusion Expression System from Invitrogen
5. R.A.P.I.D. System from Idaho Technology Inc.
6. CEQ 8800 Genetic Analysis System from Beckman Coulter
7. ProteomeLab PA 800 Protein Characterization System from Beckman Coulter
8. ProteomeLab PF 2D Protein Fractionation System from Beckman Coulter
9. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
10. CEQ 8000 Genetic Analysis System from Beckman Coulter
11. LCMS-IT-TOF Formula Predictor System from Shimadzu
... The kit was tested on rat tissue extracts ... yeast ( S. cerevisae ) extract. It was ... tissues and cell lines. Application: A simple and ... fractions based on the detection of the inorganic ...
... CAST Equine EIA Principle: ... LTC4, LTD4 and LTE4 are ... by 5-lipoxygenase and glutathione-S-transferase. In ... termed "Slow Reacting Substances of ...
... stress is the condition in which a ... stimuli and the various antioxidants. Oxidative ... pathogenesis of several diseases, including artherosclerosis, chronic ... possesses several antioxidant systems that are very ...
... tested through 63 ligand ... spanning a broad therapeutic ... includes many non human ... non-selective assays. Used ...
Biology Products:
(Date:9/2/2014)... team of University of Maryland physicists has published ... say make possible new nanostructures and nanotechnologies with ... quantum computing advances to new sensor development. , ... Nature Communications the Maryland scientists, primary discovery ... nanostructures that uses a connector, or "intermedium," nanoparticle ...
(Date:9/2/2014)... always true that digital distribution of media will ... means, at least when file sizes are large. ... in Yale,s Journal of Industrial Ecology ... for consoles such as PlayStation3. Researchers found that ... greenhouse gas emissions than game files downloaded over ...
(Date:9/2/2014)... study provides evidence that physical barriers formed ... argue that the same factor responsible for much ... also responsible for the linguistic diversity in the ... interaction between speech communities. Therefore, spatially isolated languages ... reduction of linguistic contact. , "Charles Darwin would ...
Breaking Biology News(10 mins):New synthesis method may shape future of nanostructures, clean energy 2New synthesis method may shape future of nanostructures, clean energy 3
... power of viruses is well documented in human history. ... the great Goliaths of human civilization, most famously in ... European conquest and settlement. In recent years, there ... effect of the pandemics in the Americas wasn,t confined ...
... information is a basic property of biological systems. Common ... Transmission of signals occurs at an even ... molecules, which bear a phosphate or a sulfate group. ... are of supreme importance, they have been extensively studied ...
... often focused on its potential effects on coral reefs, ... may be more significant, according to Donald Potts, a ... of California, Santa Cruz, and an expert in coral ... give an invited talk on "Geobiological Responses to Ocean ...
Cached Biology News:New World post-pandemic reforestation helped start Little Ice Age, say Stanford scientists 2New World post-pandemic reforestation helped start Little Ice Age, say Stanford scientists 3Sulfurous ping-pong in the urinary tract 2Sulfurous ping-pong in the urinary tract 3Ocean acidification could have broad effects on marine ecosystems 2
(Date:9/2/2014)... , Sept. 2, 2014 Orexigen Therapeutics, ... the U.S. Patent and Trademark Office (PTO) has issued ... being evaluated for weight loss. NB32 is a fixed-dose ... U.S. Patent No. 8,815,889 claims methods for ... bupropion. The patent expires in 2024. If NB32 is ...
(Date:9/2/2014)... September 02, 2014 PMG Research, ... 11th clinical research site: PMG Research of Rocky Mount. ... operation of the clinical research department at Boice-Willis ... presence in eastern North Carolina. This new site enhances ... patients in Rocky Mount and the surrounding communities, this ...
(Date:9/1/2014)... (PRWEB) September 01, 2014 The ... $1,712.1 million by 2019 from $1,070.7 million in ... 2014 to 2019. The global oligonucleotide synthesis market ... services, applications, end users, and geography. The synthesized ... growth rate in the oligonucleotide synthesis market during ...
(Date:8/31/2014)... 2014 This is a professional ... the Epoxy Hardener industry in Global and ... the industry, including definitions, applications and industry chain ... analysis are provided with a focus on history, ... A comparison between the international and Chinese situation ...
Breaking Biology Technology:Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 2Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 3Orexigen Expands NB32 Intellectual Property Portfolio With New U.S. Patent 4PMG Research Announces the Formation of its Eleventh Site 2PMG Research Announces the Formation of its Eleventh Site 39.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 29.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 39.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 49.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 5Epoxy Hardener Industry Development Trends in Global & China Regions Now Available at ReportsnReports.com 2Epoxy Hardener Industry Development Trends in Global & China Regions Now Available at ReportsnReports.com 3