|Products||microRNA Expression Profiling - Comprehensive Service from LC Sciences|
|Item||microRNA Expression Profiling - Comprehensive Service|
|Description|| LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using Paraflo technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs (1-3). We have standard arrays for mature miRNA of all species available in the latest version of the Sanger miRBase database (Release 12.0, September 2008). Our service is comprehensive and includes small RNA enrichment from your total RNA sample, single or dual color labeling, hybridization, image data processing and data analysis. Two-three weeks after receiving your total RNA samples, we send you the analyzed data, representative and original images of the array, and raw data. We offer additional products and services including arrays containing miRNA*, walk/tiling arrays, custom arrays (first 100 probes free of charge), and in-depth analysis for multiple data sets. The optional data analysis may include multi-array normalization, t-test, ANOVA, False Discovery Rate calculator, and/or clustering analysis.|
LC Sciences is a genomics and proteomics company offering innovative, customizable, and comprehensive microarray services for nucleic acid/protein profiling and functional analysis, biomarker-discovery, novel drug screening, and the custom development of diagnostic devices. We provide unique one-stop products and services for assays of DNA, RNA, protein, enzymes, antibodies, or small molecules, as well as proven microfluidic technology and novel microarray chemistry for design of your miniaturized assay devices for diagnostics and biosensing applications.
Our microarray services include the use of custom synthesized microarrays based on the Paraflo on-chip synthesis technology which enables the total customization of content on each individual microarray. Our customers have the flexibility to use our standard probe content or their own custom designed probe content and to use probes of their chosen lengths and number of replicates. The Paraflo technology also provides the flexibility of synthesizing microarrays containing probes with modifications such as, 5-amino linker, phosphate, biotin, and various methylation or other types of modifications. Incorporation of modifications greatly increases the number of applications for these microarrays.
LC Sciences offers additional Paraflo technology derived products that serve customers needs for construction of biomolecular libraries containing up to millions of different biomolecules of defined sequences. These products significantly reduce the cost and increase the speed of highly multiplexing genome-scale experiments such as exploration of small RNAs and designer gene/DNA library synthesis.
(1) Vagin VV, Sigova A, Li C, Seitz H, Gvozdev V, Zamore PD. (2006) A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline. Science 313(5785), 320-324.
(2) Saito, Y., Liang, G., Egger, G., Friedman, JM., Chuang, JC., Coetzee, GA., Jones, PA. (2006) Specific activation of microRNA-127 with downregulation of the protooncogene BCL6 by chromatin-modifying drugs in human cancer cells. Cancer Cell 9(6), 435-443.
(3) Cummins, J.M., He, Y., Leary, R.J., Pagliarini, R., Diaz, L.A., Jr., Sjoblom, T., Barad, O., Bentwich, Z., Szafranska, A.E., Labourier, E. et al. (2006) The colorectal microRNAome. Proc Natl Acad Sci USA 103, 3687-3692.
|Info|| LC Sciences|
2575 W. Bellfort
Houston, TX 77054
Fax Number: (713) 664-8181
Web Site: http://www.lcsciences.com