Navigation Links
loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

ProductsloxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
Company Gene Bridges GmbH
Item loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP)
Price 
Description The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells.
The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique NotI and XhoI sites flank the cassette for convenient cloning with restriction sites.
Using the provided PCR template one can easily create a loxP-amp-loxP cassette flanked by any other restriction sites to clone the cassette into the vector of choice. The restriction sites can be introduced by adding the corresponding sequence in the PCR primer. The template can easily be used to engineer the E.coli genome by Red/ET Recombination.
The loxP-amp-loxP cassette is not linear but plasmid based (3502 bp in size). Due to its R6K origin the plasmid cannot replicate in most E.coli strains. The PCR product can therefore be used directly for downstream applications without any further purification.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
2. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
3. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
4. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
5. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
6. Mouse Anti-Ampicillin Monoclonal Antibody, Unconjugated, Clone 164-12-2 from Abcam
7. EasySep Biotin Selection Kit, Other from StemCell Technologies, Inc.
8. EasySep PE Selection Kit, Other from StemCell Technologies, Inc.
9. EasySep PE Selection Kit, Other, Starter Kit from StemCell Technologies, Inc.
10. EasySep FITC Selection Kit, Other from StemCell Technologies, Inc.
11. FailSafe PCR PreMix Selection Kit from EPICENTRE Biotechnologies
...
Coverslips for Microarrays, 1. *Category: MICROARRAY ACCESSORIES....
S. O'Hare & C.K. Atterwill • In vitro toxicity techniques for regular lab use. Ex vivo techniques are also covered with the aim of reducing and refining the use of animals in the future....
... The ScriptCap™ 2'-O-Methyltransferase converts Cap 0 (or ... The Cap 1 structure has been shown ... up to 50%. This improvement is seen ... ScriptCap™ m7G Capping System as well as ...
Biology Products:
(Date:7/30/2014)... could be as simple as inserting a medicated, disappearing ... Washington bioengineers have discovered a potentially faster way to ... HIV. Their method spins the drug into silk-like fibers ... higher doses of the drug than possible with other ... could offer women a potentially more effective, discreet way ...
(Date:7/30/2014)... out how to create colors, nature had already perfected ... many different hues, for example. Now scientists are tapping ... way to make colored plastics. Their paper on using ... rather than dyes, to produce color appears in ... Asger Mortensen, Anders Kristensen and colleagues point out that ...
(Date:7/30/2014)... Monash University Professor Steven Chown has been awarded ... Medal for Excellence in Antarctic Research. , SCAR, ... coordinates high quality international scientific research in the ... Antarctic region in the Earth system. The organisation ... Antarctic Treaty System, responsible for governing the region. ...
Breaking Biology News(10 mins):Dissolvable fabric loaded with medicine might offer faster protection against HIV 2Dissolvable fabric loaded with medicine might offer faster protection against HIV 3
... SANTA CRUZ, CA--Studies conducted in California and elsewhere ... a tool for managing fisheries and protecting marine habitats, ... Cruz. A recent study in the Gulf ... key concept behind marine reserves--the idea that offspring produced ...
... For parents of children with cancer, the hopeful ... for decades. Among the bad newstreatments that enable survival ... cancer who have reached puberty currently have an opportunity ... may have their semen frozen and preserved in sperm ...
... David Queller and Joan Strassmann argue in a new paper ... genetic level on up, give a living thing its "organismality," ... or a colony. Some of the traits scientists ... membership in the same species, may not be necessary to ...
Cached Biology News:Studies show marine reserves can be an effective tool for managing fisheries 2Studies show marine reserves can be an effective tool for managing fisheries 3Studies show marine reserves can be an effective tool for managing fisheries 4Studies show marine reserves can be an effective tool for managing fisheries 5For young boys with cancer, testicular tissue banking may be option to preserve fertility 2For young boys with cancer, testicular tissue banking may be option to preserve fertility 3What is the meaning of 'one'? 2What is the meaning of 'one'? 3
(Date:7/30/2014)... Mass. , July 30, 2014 /PRNewswire/ ... ), a biotechnology company focused on discovering, ... unmet medical needs using RNA-targeted technologies, today ... (RXI-109-1402) with RXI-109, for the reduction of ... revision surgery, has been initiated. ...
(Date:7/30/2014)... (PRWEB) July 30, 2014 Adding to ... weighing boat special – Buy 2 of ... This promotion will benefit lab technicians looking for durable ... boats are ideal for multiple applications including weighing liquids, ... variety of choices including diamond-shaped, hexagonal, square, aluminum weighing, ...
(Date:7/29/2014)... 30, 2014 “PharmaSphere: Emerging Biotech ... Venture Capital Funding” report provides in-depth analysis of ... equity and venture capital segments of the financial ... to have significant impact on the future of ... Furthermore, the report identifies regions of the world ...
(Date:7/29/2014)... Calif. , July 29, 2014 Human ... and therapeutic company focused on extending the healthy, high ... Franz Och , Ph.D., an expert in machine ... Scientist. Och comes to HLI from Google where he ... Translate. He will report directly to HLI Co-Founder and ...
Breaking Biology Technology:RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 2RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 3RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 4RXi Pharmaceuticals Expands Clinical Program with Initiation of Third Phase 2a Study with RXI-109 for Treatment of Hypertrophic Scars 5Weighing Boat Promotion Now Available at Pipette.com, Major Pipette Distributor 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 3Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 4Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3