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Z-Competent E.coli Transformation Buffer Set from Zymo Research

ProductsZ-Competent E.coli Transformation Buffer Set from Zymo Research
Company Zymo Research
Item Z-Competent E.coli Transformation Buffer Set
Price $99.00
Description The Z-Competent E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Z-Competent method completely eliminates the requirement for heat shocking and related procedures. Instead, Fast-Track bacterial transformation can be performed by adding DNA to Z-Competent cells and incubating on ice for 10-20 minutes. Transformation efficiencies are typically on the order of 10^8-10^9 transformants/g of transformed plasmid DNA with most E. coli strains. Z-Competent E. coli cells also demonstrate fast transformation kinetics during incubation on ice. Most E. coli strains respond well to the Z-Competent preparation method. Uniquely formulated reagents make it easy to generate Z-Competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Z-Competent E. coli Transformation Kit includes all buffers and ZymoBroth medium to generate 20 ml of Z-Competent cells. The Z Competent E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Z-Competent cells.
Info Zymo ResearchZymo Research
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