Navigation Links
UBC Fractions Extract from GeneTex

ProductsUBC Fractions Extract from GeneTex
Company GeneTex
Item UBC Fractions Extract
Price $440.00
Description Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelmans syndrome, and Liddle syndrome. The enzyme fractions supplied represent the full compliment of purified conjugation enzymes (E1, E2s, and E3s) that are found in mammalian Fraction II. The enzymes have been tested and shown to work with typical [125I]-labeled substrate proteins such as lysozyme and beta-lactoglobulin. These conjugation fractions contain ubiquitin c-terminal hydrolases. The addition of ubiquitin aldehyde is recommended for the inhibition of UCHs and to improve overall conjugate yield. The supplied fractions do not contain 20S or 26S protein degradation activity. If the substrate being conjugated requires E2 or E3 enzymes not found in Fraction II, the reaction can be supplemented with enzymes from Fraction I. Includes complimentary fractions for 2-5 conjugation reactions depending on conditions.

  • Type: Proteins and peptides
  • Form: 400 g in 50 mM HEPES, pH 7.6, containing 1 mM DTT.
Info GeneTexGeneTex
14785 Omicron Dr. Suite101
San Antonio, TX 78245
Customer Service: (877) 436-3839
Fax Number: (210) 677-8843
Web Site: http://www.genetex.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Human CALCA EIA (Enzyme immunoassay) Kit, Host: Rabbit ; Extraction-free from BACHEM
2. 26S Proteasome Fraction Extract from GeneTex
3. Extracti-Gel D Detergent Removing Gel AffinityPak Columns from Pierce Biotechnology, Inc.
4. Ubiquitin/Proteasome Fraction II Extract from GeneTex
5. 26S Proteasome Fraction Extract from GeneTex
6. IRS Extract from GeneTex
7. Ubiquitin Fraction I Extract from GeneTex
8. Feature Extraction Software; 2-year License for Not-For-Profit/Academic from Agilent Technologies
9. Feature Extraction Software; 1-year license for Commercial from Agilent Technologies
10. Feature Extraction Software; 1-year License for Not-For-Profit/Academic from Agilent Technologies
11. Panorama Mouse/Rat Tissue Extract Protein Array Kit from Sigma-Aldrich
Procollagen type I C-terminal propeptide (human, PICP)...
... in Early Drug Development with Imaging Mass ... drug compound localization data in early drug ... mass spectrometry service maps unlabeled ... so you can localize drugs and metabolites ...
... LC Sciences provides a unique new ... DNA synthesis serving diverse needs in genomics, ... applications. OligoMix is a MIXTURE of a ... sequences delivered in a single microtube. OligoMix ...
... is an automated, high-resolution protein analysis ... ProteomeLab family of products, the PA ... automated protein characterization solutions including molecular ... carbohydrate profiling and mechanisms to study ...
Biology Products:
(Date:4/23/2014)... in their mid-thirties and forties, face a devastating ... two decades. "Mutant" protein clusters, long blamed for ... the primary focus of therapies in development by ... Prof. Gerardo Lederkremer and Dr. Julia Leitman of ... Immunology, in collaboration with Prof. Ulrich Hartl of ...
(Date:4/23/2014)... Toronto Scarborough research shows that male black widow spiders ... a rare example of mate preference by male spiders. ... Emily MacLeod and Maydianne Andrade, a professor in UTSC,s ... studies and the wild that males overwhelmingly chose to ... black widows can tell whether a potential mate is ...
(Date:4/23/2014)... source of a unique rhythmic sound, recorded for decades ... the Antarctic minke whale ( Balaenoptera bonaerensis ). First ... who thought it sounded like a duck, the bio-duck ... Southern Ocean, but its source has remained a mystery, ... of researchers deployed acoustic tags on two Antarctic minke ...
Breaking Biology News(10 mins):On the defensive 2On the defensive 3Picky male black widow spiders prefer well-fed virgins 2Scientists identify source of mysterious sound in the Southern Ocean 2Scientists identify source of mysterious sound in the Southern Ocean 3
... have uncovered a dramatic new twist in the battle against ... itself immune to a key component of the only effective ... team of TB researchers at Leicester announced a new advance ... They isolated the molecular weapons of the bacterium and are ...
... spring arrives across the country, tourists returning to beaches will ... algae that make water unfit for swimming and pose risks ... realize is that the small streams running through their neighborhoods ... feeds the algae blooms. A new study published in ...
... RIDGE, Tenn., March 12, 2008 -- Noted green energy ... this years Global Venture Challenge, a business competition and ... Ridge National Laboratory. The focus of this years ... bioenergy, energy efficiency and power generation sectors. Sridhar, ...
Cached Biology News:Scientists discover how TB 'develops invincibility' against only available treatment 2From the backyard to the ocean: New study shows streams act as key nitrogen filters 2
(Date:1/15/2014)... "The interface is the device," Nobel laureate Herbert ... to be found at the junctures where layers ... of nanotechnology, the interfaces between layers of metal ... such high-tech favorites as spintronics, high-temperature superconductors, ferroelectrics ...
(Date:1/15/2014)... 15, 2014 Cynvenio, a cancer diagnostics ... the genomic analysis of tumor cells in the bloodstream, ... web-based cancer decision support application powered by CollabRx, Inc. ... ClearID™ genomic test, Therapy Finder will provide breast cancer ...
(Date:1/15/2014)... More than 5 million Americans are currently ... seniors will die with Alzheimer’s or another dementia, according ... shocked many Americans into looking for ways to improve ... age-related cognitive disorders. Jonathan Weisman, president of Biohack Pure, ...
(Date:1/15/2014)... Freeslate, Inc ., the leading ... Lupin Limited, one of India’s top five pharmaceutical companies, ... System for high throughput solid form screening. ... a wide range of quality, affordable generic and branded ...
Breaking Biology Technology:A deeper look at interfaces 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3