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Tritirachium album Proteinase K from USB Corp.

ProductsTritirachium album Proteinase K from USB Corp.
Company USB Corp.
Item Tritirachium album Proteinase K
Description Source: Tritirachium album
Description:Proteinase K is a highly active and stable endopeptidase used in a wide range of applications purified from the fungus T. album. It cleaves peptide bonds mainly following the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids(1) and is classified as a serine protease. Proteinase K is useful in purifying high molecular weight nucleic acids from cells and tissues (i.e. genomic DNA isolation from mouse tails) and in revealing protein structure and function.
Tested User Friendly
Molecular Weight: 28,900 Da
Optimum pH: 4.0-12.5(1)
Optimum Temperature: 65C(1)
Working Concentration: 0.05 - 1 mg/ml
Activators: Stimulated by 0.2 - 1% SDS or 1 - 4M urea(8)
Inactivated by PMSF(1); Not inactivated by EGTA, thiol reagents or trypsin/chymotrypsin inhibitors(1)
Form:Lyophilized powder
Purity:Tested for contaminating ribonucleases and deoxyribonucleases.
Shipping and Storage:Shipped on dry ice. Store dry at 4C.
Unit Definition:One unit is the amount of enzyme that liberates folin positive amino acids and peptides corresponding to 1 mol tyrosine in 1 min at 37C using hemoglobin as substrate.
Concentration:20 - 50 units/mg
Applications:Used in the isolation of native, high molecular weight DNA and RNA.
*Used as a general protease to inactivate DNases, RNases and to degrade proteins.
*Specifically modifies cell surface proteins and glycoproteins for analysis of membrane structures for protein localization(5,6).
*Produces characteristic protein fragments used in enzyme/protein structure and function studies(7).
*Improves the cloning efficiency of PCR products.
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128

Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-216-245-1829
Customer Service: 216.765.5000
Fax Number: 216.464.5075
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NOTE: Price information is approximate list price and actual prices may vary.

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