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Tritirachium album Proteinase K from USB Corp.

ProductsTritirachium album Proteinase K from USB Corp.
Company USB Corp.
Item Tritirachium album Proteinase K
Price 
Description Source: Tritirachium album
Description:Proteinase K is a highly active and stable endopeptidase used in a wide range of applications purified from the fungus T. album. It cleaves peptide bonds mainly following the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids(1) and is classified as a serine protease. Proteinase K is useful in purifying high molecular weight nucleic acids from cells and tissues (i.e. genomic DNA isolation from mouse tails) and in revealing protein structure and function.
Tested User Friendly
Properties:
Molecular Weight: 28,900 Da
Optimum pH: 4.0-12.5(1)
Optimum Temperature: 65C(1)
Working Concentration: 0.05 - 1 mg/ml
Activators: Stimulated by 0.2 - 1% SDS or 1 - 4M urea(8)
Inactivated by PMSF(1); Not inactivated by EGTA, thiol reagents or trypsin/chymotrypsin inhibitors(1)
Form:Lyophilized powder
Purity:Tested for contaminating ribonucleases and deoxyribonucleases.
Shipping and Storage:Shipped on dry ice. Store dry at 4C.
Unit Definition:One unit is the amount of enzyme that liberates folin positive amino acids and peptides corresponding to 1 mol tyrosine in 1 min at 37C using hemoglobin as substrate.
Concentration:20 - 50 units/mg
Applications:Used in the isolation of native, high molecular weight DNA and RNA.
*Used as a general protease to inactivate DNases, RNases and to degrade proteins.
*Specifically modifies cell surface proteins and glycoproteins for analysis of membrane structures for protein localization(5,6).
*Produces characteristic protein fragments used in enzyme/protein structure and function studies(7).
*Improves the cloning efficiency of PCR products.
Info USB Corp.USB Corp.
26111 Miles Road
Cleveland Ohio 44128


Call USB Corp. to buy products (US and Canada only; other locations use numbers below)
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Customer Service: 216.765.5000
Fax Number: 216.464.5075
Web Site: http://www.usbweb.com
NOTE: Price information is approximate list price and actual prices may vary.

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