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TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem

ProductsTACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
Company Calbiochem
Item TACE Fluorometric (MCA)) Assay Kit, InnoZyme™
Price $442.00

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96 Tests. PROTECT FROM LIGHT. AVOID FREEZE/THAW CYCLES. The InnoZyme™ TACE Activity Kit is a specific and sensitive assay designed to measure human TACE activity in cell lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well Plate is pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an excitation wavelength of ~324 nm and emission wavelength of ~400 nm. The level of fluorescence is directly related to the enzyme activity.

Kirkegaard, T., et al. 2004. Clin. Exp. Immunol. 135, 146.Neumann, U., et al. 2004. Anal. Biochem. 328, 166.Skovronsky, D.M., et al. 2001. J. Neurobiol. 49, 40.Black, R.A., et al. 1997. Nature 385, 729.Moss, M.L., et al. 1997. Nature 385, 733.
Info CalbiochemCalbiochem
Calbiochem, A Brand of EMD Biosciences, Inc.
10394 Pacific Center Court
San Diego, CA 92121

Customer Service:
(800) 854-3417
(858) 450 9600

Tech Support: 800-628-8470
Fax Number:
(800) 776-0999
(858) 453 3552

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