|Products||Reverse-iT One-Step Kit, ReddyMix from ABgene|
|Item||Reverse-iT One-Step Kit, ReddyMix|
|Description|| The Reverse-iT One-Step Kit is the ultimate in convenience, sensitivity and reproducibility in RT-PCR. It has been specifically designed to streamline the RT-PCR process.|
The Reverse-iT One-Step Kit combines the unique Reverse-iT Blend of RTases and ABgene®s Thermoprime Plus DNA Polymerase to provide a highly sensitive, reproducible and easy-to-use system for the detection and analysis of RNA. The kit is able to detect RNA from as little as 100 femtograms. The Reverse-iT Blend can be used at temperatures up to 57°C, avoiding problems caused by secondary structure in the RNA template. It also shows a better dynamic range than AMV or MMuLV. This means that increases in template concentration give rise to corresponding increases in product generated over a wider range of starting material concentrations.
The master mix format of the kit minimises reagent handling steps, reducing errors and the risk of contamination. It is supplied at a 2x concentration, providing extra flexibility for primer and template concentrations, and contains a proprietary buffer, which has been optimised for exceptional sensitivity and yield. The Reverse-iT Blend is provided in a separate tube, allowing negative controls without RTase to be carried out easily. The kit is also available in ABgene®s ReddyMix format, which contains a dye and precipitant for running samples directly on electrophoresis gels after RT-PCR.
Surrey KT19 9AP
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Fax Number: +44 (0)1372 741414
Web Site: http://www.abgene.com