Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
Phosphotyrosine IgG mouse monoclonal antibody: Alkaline Phosphatase conjugate...
Rabbit polyclonal to GPCR G2A ( Abpromise for all tested applications). entrezGeneID: 29933 SwissProtID: Q9UNW8...
... reagent has been developed for use ... reduction of nonspecific background staining due ... describe blocking peroxidase with hydrogen peroxide. ... in combination with additional novel components ...
Rabbit polyclonal to Wilms Tumor Protein ( Abpromise for all tested applications). entrezGeneID: 7490 SwissProtID: P19544...
Biology Products:
(Date:8/21/2019)... ... 20, 2019 , ... Scientists ranked New England Biolabs number ... the survey in 2016. The results are published in the newest report, ... Science and Medicine Group. More than 800 life scientists were asked to rank ...
(Date:8/15/2019)... ... August 15, 2019 , ... Deep Science Ventures ... Food and Agriculture practice, a new unit dedicated to building 100 companies over the ... raw materials and supply chains. , Deep Science Ventures was founded to create ...
(Date:8/15/2019)... ... August 15, 2019 , ... Make IVD tests ... routine tasks. That was the message at the world's largest gathering of laboratory ... American Association for Clinical Chemistry (AACC) Annual Scientific Meeting was held in Anaheim, ...
Breaking Biology News(10 mins):
(Date:8/8/2019)... ... August 08, 2019 , ... ... sales and service organizations, today announced that the company’s Studio Intelligent Virtual Agent ... IVA market . Inference will officially accept the award at Frost & Sullivan’s ...
(Date:8/6/2019)... DEERFIELD, Ill. (PRWEB) , ... August 05, 2019 ... ... announced that Jason Casarella has been recognized as a distinguished leader by PharmaVOICE ... is featured in an article in the magazine’s July/August special issue. , As ...
(Date:7/26/2019)... ... 25, 2019 , ... LeadingBiotech , an exclusive event ... its Boston BD conference to be held on September 10, 2019, ... , the event provides an opportunity for attendees to leverage insight from real-life ...
(Date:7/17/2019)... COLLEGE PARK, Md. (PRWEB) , ... July 15, 2019 , ... ... cut DNA so that a certain trait can be removed, replaced, or edited, but ... Maryland, is looking far beyond these traditional applications in his latest publication in Nature ...
Breaking Biology Technology: