Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
(Date:7/22/2014)... most kids will hear that drinking milk helps make their bones ... these messages foster the idea that if something is good for ... in the Journal of Consumer Research , when children hear ... it. , "We predicted that when food is presented to children ... goal such as learning how to read or count, they would ...
(Date:7/22/2014)... MSN, APRN, PMHCNS-BC, CNE, Assistant Professor of Clinical ... School of Nursing, contributed samples used in a ... with schizophrenia and also suggesting a possible link ... Biological insights from 108 schizophrenia-associated genetic loci, was ... , available at http://www.nature.com/nature/journal/vaop/ncurrent/full/nature13595.html., ...
(Date:7/22/2014)... side of meerkats which sees them prevent their daughters ... a new study. , Research into the desert creatures ... many adult helpers shows that the alpha female can ... The study shows how this way of life, also found ... effective despite its sinister side. , Dominant meerkats control ...
Breaking Biology News(10 mins):LSUHSC contributes to work identifying new DNA regions associated with schizophrenia 2
... Many of society,s most challenging health issues, including obesity, ... University of Missouri researchers are examining the multiple facets ... from medicine and research to policy and law to ... in the Mizzou Advantage (During a three-year process, MU ...
... of the Key West, Fla., population over 1,000 people ... 2009, according to a report from the Centers for Disease ... After three initial locally acquired cases of dengue were ... Department of Health conducted a study to estimate the potential ...
... Detecting oral cancer in its earliest stages can save ... early detection has been difficult. Case Western Reserve ... human beta defensin-3 (hBD-3), which may serve as an early ... and associated with the early stages of oral cancer. ...
Cached Biology News:Food for our future -- it's more than what's for dinner 2Food for our future -- it's more than what's for dinner 3Report suggests nearly 5 percent exposed to dengue virus in Key West 2CWRU dental researchers discover human beta defensins-3 ignite in oral cancer growth 2
(Date:7/22/2014)... Parsippany, NJ (PRWEB) July 22, 2014 ... analytical, precision, portable, and mechanical balances and scales, ... precision balances has won Laboratory Equipment magazine’s 2014 ... is the second consecutive year the Explorer was ... readers. The annual Readers’ Choice Awards celebrates “excellence ...
(Date:7/22/2014)... and SHENZHEN, China , ... Co., LTD, (BGI Tech), a subsidiary of BGI, ... global launch of a new human whole exome ... This newly offered service includes in-depth bioinformatics analysis ... prices until December 31, 2014. ...
(Date:7/22/2014)... (PRWEB) July 22, 2014 Quorum Review IRB, ... service for large clinical trials called Quick Step™ . ... designed to address the research industry’s need for seamless study ... service is available for studies planning 50 or more North ... combined planned North American site count of 80 or more. ...
(Date:7/22/2014)... 2014 The next session of ... and will comprise of the increasingly popular ‘Base SAS’ ... is a series of technical hands-on classes for entry-level ... reach the next step in their professional careers. , ... by Jennifer Kang, Senior Statistician at Genentech and former ...
Breaking Biology Technology:OHAUS Explorer Voted “Best Balance” in Laboratory Equipment’s 2014 Readers’ Choice Awards 2BGI Tech Launches Human Whole Exome Sequencing Service on Complete Genomics' Advanced Platform 2BGI Tech Launches Human Whole Exome Sequencing Service on Complete Genomics' Advanced Platform 3Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 2Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 3Clinovo TechTrainings: Next Session of SAS and CDISC Classes Start 9th September, 2014 2Clinovo TechTrainings: Next Session of SAS and CDISC Classes Start 9th September, 2014 3