Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
BD Falcon microtest assay plate. 1536 well 12l, black BD Optilux PS. Polystyrene, nonsterile, no lid (15/sp, 60/ca) Packaging: 15 / pack, 60 / case ...
... washing needs in both the 384-well format and ... an excellent solution for a variety applications in ... or Lead Optimization) and Research. , Unmatched ... set a standard for ultra-fast washing in both ...
... MULTI-FUN Incubator is a compact ... uses a microprocessor controller with a ... temperature and agitation speed. Air circulation ... the incubator. Temperature can be easily ...
... emerging as a powerful new tool for ... only amino groups have been able to ... as our Sulfo-SBED. Sulfhydryl-reactive Mts-Atf-Biotin and its ... to the protein interaction tool chest that ...
Biology Products:
(Date:2/14/2020)... ... February 14, 2020 , ... Trial By Fire Solutions, ... and Payment tracking, will be showcasing SimpleTrials at the Summit For Clinical Ops ... SCOPE Summit last year, the SimpleTrials team has completed full validation of the ...
(Date:2/13/2020)... ... February 13, 2020 , ... Murrieta Genomics , ... be hosting a lunch and learn event on February 25th from 11:30 am to ... and interested members of the public are invited to attend this free event, but ...
(Date:2/13/2020)... ... 13, 2020 , ... Strategic Directions International (SDi) announced today ... Life Science Instrumentation Industry. The report will be released on February 28th. The ... the $70 billion laboratory market for analytical and life science instruments. Special offers ...
Breaking Biology News(10 mins):
(Date:2/19/2020)... ... 2020 , ... Shoreline Biome, a microbiome research ... recently unveiled a novel DNA isolation product. , Shoreline Rapid Prep is a ... weight (HMW) single-stranded DNA (ssDNA) in excess of 40 kB from Gram-negative bacteria, ...
(Date:2/19/2020)... ... February 18, 2020 , ... Intech , the leader ... new and unified global brand identity, as it celebrates its 20th anniversary. , ... well as an enthusiasm for designing and manufacturing state-of-the-art medical devices, Intech has ...
(Date:2/19/2020)... YARDLEY, Pa. (PRWEB) , ... February 19, 2020 ... ... performance, and process solutions for the life sciences industry, today announced the opening ... by Kenji Nagamura, Associate Partner, Red Nucleus R&D. , Red Nucleus announced the ...
(Date:2/13/2020)... ... February 12, 2020 , ... ... (PV) solutions and services, is adopting Veeva Vault Safety™ as part ... requirement for fast assessment of data, processing of safety information such as SUSARs ...
Breaking Biology Technology: