Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
The imaging screen-K (Kodak), tritium, is a 20 x 25 cm storage phosphor screen that is used for the quantitation and imaging of 32P, 33P, 35S, 14C, and 3H....
The filter 695RDF55 Reflect 635 for the Molecular Imager FX is a band pass filter for Cy5 and Alexa Fluor 635 dyes....
The filter 605DF50 for the Molecular Imager FX is a band pass filter for ethidium bromide, SYPRO Red, SYPRO Ruby, Alexa Fluor 532 and 546, and Cy3 dyes when using the 635 nm laser....
... tube gel cell is a stand-alone apparatus ... electrophoresis applications. The upper buffer chamber can ... cm long for isoelectric focusing (IEF) applications. ... and cooling core, lower buffer chamber, lid ...
Biology Products:
(Date:7/21/2014)... to monitor seals, every movement, researchers have shown for ... to offshore wind farms and pipelines. Those man-made structures ... according to a study published in the Cell Press ... , "I was shocked when I first saw the ... Shoal," an offshore wind farm in the United Kingdom, ...
(Date:7/21/2014)... Want to conserve water and save on your utility ... and published in the current issue of the journal ... List: The Most Effective Actions U.S. Households Can Take ... water use substantially by simple actions such as installing ... consumption. While the suggestions may be familiar, the researchers ...
(Date:7/21/2014)... Dysfunction in dopamine signaling profoundly changes the ... brain,s prefrontal cortex and may be an underlying ... schizophrenia, according to UC Irvine scientists. , This ... that receive this neurotransmitter showed for the first ... of behavioral and physiological functions regulated in the ...
Breaking Biology News(10 mins):Seals forage at offshore wind farms 2Researchers provide guide to household water conservation 2Researchers provide guide to household water conservation 3UCI researchers find epigenetic tie to neuropsychiatric disorders 2
... things that suffer from stress. Trees must deal with stress ... too much water, from scarcity of a needed nutrient, from ... to stress quickly and efficiently is a pressing goal of ... University scientists has identified the molecular mechanism that Populusthe scientific ...
... A recent research study has shown that food ... food due to resource constraints, can lead to greater ... "Prior studies have shown that women living ... health complications," said Craig Gundersen, a University of Illinois ...
... scientists have analysed the temperature and salt levels of the ... evolution of each variable. Their research shows that, since at ... and saltier, and that, since the 1990s, this process has ... layer of the Western Mediterranean increases by 0.002C, and its ...
Cached Biology News:Feeling stressed? So is the poplar 2Food insecurity increases risk of weight gain and complications during pregnancy 2Temperature and salt levels of the Western Mediterranean are on the increase 2
(Date:7/22/2014)... Angeles, CA (PRWEB) July 22, 2014 ... test solutions with the debut of its fluorescent ... of Clinical Chemistry (AACC) meeting in Chicago, July ... or bench top versions, the HRDR-300 is a ... pending technology to analyze fluorescent lateral flow immunoassays ...
(Date:7/21/2014)... July 21, 2014 Lawrence ... Investment Board (WIB) and Las Positas College (LPC) ... technical education and hands-on training for veterans, officials ... Program at Las Positas is designed to ... for engineering technician careers, and establishes a pipeline ...
(Date:7/21/2014)... PHILADELPHIA , July 21, 2014  Mike ... closing of $1.6 million in Series Seed B ... and chemical sensors for the life science, defense, ... Trimaran Capital Partners led the round and was ... 36 Angels.  Said Patterson , "This round ...
(Date:7/19/2014)... 20, 2014 United States Non-Vascular ... GlobalDatas new report, "United States Non-Vascular Stents Market ... the United States Non-Vascular Stents market. The report ... (in units) and average prices (in US dollars) ... Stents and Metal Biliary Stents (Metal Non-Covered Biliary ...
Breaking Biology Technology:Holomic to Debut HRDR-300 Fluorescent Immunoassay Reader at the AACC 2014 2Engineering Program Established for Veterans 2Engineering Program Established for Veterans 3Global Graphene Manufacturing Leader, Graphene Frontiers, Receives Series Seed B Funding To Assure Production Of The Next Generation Of Biological And Chemical Sensor Technology 2United States Non-Vascular Stents Market Outlook to 2020 | Researchmoz 2United States Non-Vascular Stents Market Outlook to 2020 | Researchmoz 3United States Non-Vascular Stents Market Outlook to 2020 | Researchmoz 4