Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
PA coated Agarose beads LPA Beads...
Mouse monoclonal antibody to PAEP - progestagen-associated endometrial protein (placental protein 14, pregnancy-associated endometrial alpha-2-globulin, alpha uterine protein)...
Compact, portable device, seals 96 and 384 well plates with preset temperature optimised for Eppendorf heat sealing foils and films. Suitable for plates of different heights. AC input: 50/60 Hz230 V...
Rabbit polyclonal to ErbB 2 (phospho Y1248) ( Abpromise for all tested applications). entrezGeneID: 2064 SwissProtID: P04626...
Biology Products:
(Date:7/31/2018)... SAN DIEGO (PRWEB) , ... July 31, 2018 , ... ... cell analysis. The company has just introduced the xCELLigence RTCA S16, which ... Controlled and monitored via an external computer, the S16can be placed in any standard ...
(Date:7/31/2018)... , ... July 31, 2018 , ... ... William “Will” Muzek as Business Development Manager. In this role, Mr. Muzek will ... to accelerate Lifecycle’s continued growth and expansion. , With over 20 years ...
(Date:7/30/2018)... ... July 30, 2018 , ... TC Slade, an ... of biofield energy healing treated Vitamin D3 on the strength and health of ...     Over 260% increase in ALP, a bone-specific enzyme ,     Over 240% increase ...
Breaking Biology News(10 mins):
(Date:8/14/2018)... ... August 14, 2018 , ... Buprenorphine (BUP) ... disorder. BUP is often prescribed as a sublingual co-formulation with the opioid antagonist ... webinar, participants will learn from a review of the pharmacokinetics of BUP, NBP, ...
(Date:8/9/2018)... Colo. (PRWEB) , ... August ... ... expands its industry leading Bolt-on CMC™ expert services platform by combining its ... a convenient, cost-effective approach to help you meet periodic pharmaceutical GxP training ...
(Date:8/9/2018)... ... August 08, 2018 , ... Since their establishment in ... and with a team nearly double its’ size from last year, the company is ... OpenSPR turned heads and impressed crowds at a private product launch event during ...
(Date:8/9/2018)... ... August 09, 2018 , ... Simpson Gumpertz & Heger ... with building owners and managers for a full-day discussion on health care and ... to Boston for the first time on Thursday, 13 September 2018, at Harvard ...
Breaking Biology Technology: