Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... and other fluid sources for 8 vessels ... of each individual pump is controlled electronically. ... exactly maintained. ,High-precision controlled miniature pumps ... which allows flow rates between 0 and ...
... excellent ion chromatography system . The ... from 0.01S to 5000S, houses the measuring cell ... CE 4715, is available. Data processing is ... Solvent delivery is by the high pressure CE ...
Fujifilm Pictro-Color Process for sharp, clear reproduction comparable to silver-halide photography. Photorealistic 400 dpi quality. A4 and A5 output....
... and other fluid sources for 4 vessels ... of each individual pump is controlled electronically. ... exactly maintained. ,High-precision controlled peristaltic pumps ... which allows flow rates between 0.01 to ...
Biology Products:
(Date:4/24/2014)... river turtle in North America, weighing in at up ... researchers from Florida and the University of Vermont have ... three. , Examining museum specimens and wild turtles, the ... , Once heavily hunted for turtle meat alligator ... in the 1960s the riverine populations have been ...
(Date:4/23/2014)... shows Africa,s Congo rainforest, the second-largest tropical rainforest in ... over the past decade. , The study, led by ... New York, shows between 2000 and 2012 the decline ... The research, published Wednesday in Nature , is ... the effects of long-term drought on the Congo rainforest ...
(Date:4/23/2014)... domestic heating may contribute to early fetal death according ... Research Institute of Children,s Hospital Los Angeles and Ulaanbaatar, ... , In a paper published today in the journal ... strong statistical correlations" between seasonal ambient air pollutants and ... has one of the highest levels of air pollution ...
Breaking Biology News(10 mins):Two new US turtle species described 2Two new US turtle species described 3NASA satellites show drought may take toll on Congo rainforest 2NASA satellites show drought may take toll on Congo rainforest 3Pollutants from coal-burning stoves strongly associated with miscarriages in Mongolia 2
... scientific results from an ambitious voyage led by a group ... San Diego offer a stark view of human pollution and ... been labeled as the "Great Pacific Garbage Patch." Two ... or SEAPLEX, found evidence of plastic waste in more than ...
... continuing partnership with Smithsonian Institution Scholarly Press, has converted ... to digital formats for use on Kindles, Nooks and ... unprecedented access to a vast research collection. These scholarly ... take up valuable physical space or face the threat ...
... Conservation Biology Institute and George Mason University broke ... that embodies the concept of the living classroom. ... Program has trained future conservationists with an interdisciplinary ... National Zoological Park,s 3,200-acre facility in Front Royal, ...
Cached Biology News:Scripps study finds plastic in 9 percent of 'garbage patch' fishes 2Scripps study finds plastic in 9 percent of 'garbage patch' fishes 3Smithsonian Conservation Biology Institute and George Mason University expand partnership 2
(Date:1/15/2014)... January 15, 2014 A study has been ... the Formula 1 track could help to tackle the problem ... (MAT), Stowhealth (a GP surgery based in Stowmarket) and academics ... Simplyhealth. Telemetry technology, which is inspired by equipment ...
(Date:1/15/2014)... NY (PRWEB) January 15, 2014 The Microcompetition ... a major disease. One of these latent viruses is the ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic inflammatory ... theory, a study found that RA patients have high concentrations ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Freeslate, ... solutions, today announced that Lupin Limited, one of India’s ... CM Protégé PharmD System for high throughput ... India, is focused on a wide range of quality, ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. – ... Warrington, Pa. , Details: The Hepatitis B Foundation, ... cure for hepatitis B and improving the quality of life ... on Friday, April 11 at Warrington Country Club in Warrington, ...
Breaking Biology Technology:Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2