Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
(Date:3/12/2015)... Beta Systems today announced ... management (IAM) solution for a fixed price and ... new IAM package, customers benefit from the 30 ... IAM implementations across different industries. The new fixed-price ... any necessary services and consulting. It spans the ...
(Date:3/10/2015)... NEW YORK , March 10, 2015  Continuing ... and the Unexpected, Hammacher Schlemmer introduces The Eye ... user,s identity before granting access to secure websites or ... trusted to verify travelers at international borders, the device ... 240 distinctive pattern points of the iris, converting them ...
(Date:3/5/2015)... In Brazil , cloud computing ... these solutions and 42 percent planning to invest in them ... percent of the companies will be investing in these solutions ... companies in the region are currently opting for the private ... command significant attention in the coming years. This trend is ...
Breaking Biology News(10 mins):Beta Systems Fast Forward IAM Available Now: a Standardized IAM Package at a Fixed Price 2Hammacher Schlemmer Introduces The Eye Scanning Password Authenticator 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 2Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 3Two of Every Three Brazilian Companies will have Invested in Cloud by 2015, Finds Frost & Sullivan 4
... CORVALLIS, Ore. Researchers at Oregon State University have made ... lead toward robots that not only can walk and run ... achieving an optimal approach with robotic mechanisms, studies are moving ... the military, create prosthetic limbs for humans that work much ...
... PHILADELPHIA - A team of paleontologists, including a University ... dinosaur based upon an incomplete skeleton found in western New ... rocks that preserve a swampy forest ecosystem that thrived near ... ago. The dinosaur, whose name translates to mean "grinding-mouth, ...
... considering Denmark has always been praised as the benchmark ... treatment for patients seeking ART, said Dr. Sren Ziebe ... of ESHRE and the SIG coordinator of all SIGs ... assisted reproduction treatments (ART) with up to three treatment ...
Cached Biology News:Advances made in walking, running robots 2Advances made in walking, running robots 3'Grinding mouth, wrinkle eye': Penn graduate student describes new species of plant-eating dinosaur 2
(Date:4/1/2015)... Clara, CA (PRWEB) April 01, 2015 ... Force Microscopes (AFM) was selected to receive the ... The New Economy Magazine.    Park NX10 is the premium ... XY and Z scanner linearity, closed-loop detector noise, ... feature, SmartSca n, which allows images ...
(Date:4/1/2015)... Calif. , April 1, 2015  ValGenesis Inc., ... solutions, announced today that it has completed its Service ... 101, Attest Engagements (AICPA, Professional Standards) for the period ... st 2014.  ... The SOC ...
(Date:4/1/2015)... - Portage Biotech Inc. ("Portage") (OTC Market: PTGEF, ... that Biohaven Pharmaceutical Holding Company Limited (Biohaven), which ... agreement with inVentiv Health for the operational execution ... compound (BHV-0223). Biohaven is engaged ... neuroscience compounds targeting the glutamatergic system. Clinical drug ...
(Date:4/1/2015)... April 01, 2015 Proove Biosciences ... Medicine , presented two scientific posters at the Annual ... week. The four-day event was hosted at the Salt ... dedicated to educating genetic and genomic professionals on breakthrough ... and Insomnia: Study on the Role of DAT1 in ...
Breaking Biology Technology:Park Systems Awarded the New Economy Magazine “Best Analytic Instrumentation Company 2014” 2ValGenesis Inc. Successfully Completes Service Organization Controls 2 (SOC 2) Report under AT Section 101 2ValGenesis Inc. Successfully Completes Service Organization Controls 2 (SOC 2) Report under AT Section 101 3Portage's Biohaven Announces Agreement with inVentiv Health 2Portage's Biohaven Announces Agreement with inVentiv Health 3Proove Biosciences Presents Research Studies at Annual Clinical Genetics Meeting 2015 2