Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... Lambda Protein Phosphatase ( ... protein phosphatase with activity ... and tyrosine residues. It ... product of the ORF221 ...
... Monitoring Kit measures adenosine triphosphate ... and a dynamic range. Based ... assay measures ATP in bacterial, ... a concentration range of 10-11 ...
Immulon 2HB, Flat Bottom, 50/Bx; high binding irradiated surface to provide increased binding affinity for hydrophilic proteins and complexes....
Thermo Forma provides an array of accessory Racks for the Cryo/CryoPlus 16" Diameter storage system. To maximise inventory capacity a Jumbo Arrowhead configuration is recommended. You may choose betw...
Biology Products:
(Date:9/12/2018)... ... September 12, 2018 , ... ... education, is pleased to support the professional development of academic librarians by sponsoring ... , The entrants were asked to submit successful strategies to increase collaboration among ...
(Date:9/7/2018)... ... September 06, 2018 , ... ... and sister to show Sensors Expo & Conference , announces their ... University, CA to conduct an IoT Enabled Industrial IoT Certification Program. Taking place ...
(Date:8/31/2018)... ... 2018 , ... R3 Stem Cell, the nation's leader in ... The freezers are very convenient for practices to maintain regenerative biologics on hand ... and umbilical cord tissue. They are stored at -80 degrees Celsius, which keeps ...
Breaking Biology News(10 mins):
(Date:8/29/2018)... GARDEN CITY, N.Y. (PRWEB) , ... August 28, ... ... , a leading online retailer of contact lenses, has been ranked in 1138th ... nation’s fastest growing private companies. , The list represents the most successful ...
(Date:8/26/2018)... ... August 24, 2018 , ... ExoCoBio Inc., ... dramatically relieves atopic dermatitis and inhibits a variety of inflammatory targets in the ... the symptoms of mice having severe atopic dermatitis were significantly improved after administration ...
(Date:8/17/2018)... ... August 16, 2018 , ... The opening of the new Titusville facility marks ... in 2016. Chemko had a well established local presence, servicing the Space Coast since ... such as the ongoing work at the Mobile Launch Platform (MLP) in support of ...
(Date:8/14/2018)... ... August 14, 2018 , ... Buprenorphine (BUP) is a ... is often prescribed as a sublingual co-formulation with the opioid antagonist naloxone (NXO) ... will learn from a review of the pharmacokinetics of BUP, NBP, and NXO ...
Breaking Biology Technology: