Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
Rabbit polyclonal to 3-Ethynylestradiol ( Abpromise for all tested applications). Antigen: Ethynylestradiol conjugated to BSA....
... antigen initiates replication from the ... the presence of the host ... be used in conjunction with ... cat. no. 8050. For ...
Request Info...
Anti-hamster Ig HRP detection kit...
Biology Products:
(Date:5/31/2019)... MANHATTAN, Kan. (PRWEB) , ... May 30, 2019 , ... ... all year, and especially during Military Appreciation Month, as a way to express his ... month, Patrick, and the company he founded — Enso Discoveries — presented ...
(Date:5/26/2019)... ... May 24, 2019 , ... Zolgensma (Novartis, AveXis), an AAV-delivered gene ... for clinical use in the United States by the Food and Drug Administration today. ... to treat inherited genetic disorders in the United States and the second-ever approved treatment ...
(Date:5/23/2019)... ... May 23, 2019 , ... Federal Publications Seminars, the ... is pleased to announce the release of FPSOnline 2.0, an updated version of its ... weeklong conferences , La Jolla Government Contracts Week, were given a “sneak peek” ...
Breaking Biology News(10 mins):
(Date:6/26/2019)... ... June 26, 2019 , ... Alice Branton today released research results ... properties of Magnesium Gluconate, which can prove to be beneficial for treating Hypomagnesemia. , ... differentiation peak, change in crystallite size , Over 142% increase in particle ...
(Date:6/18/2019)... ... June 18, 2019 , ... Personalized Stem Cells, Inc (“PSC”), ... to the FDA for use of a person’s own adipose-derived stem cells to treat ... in the knee. This IND is the first of several planned clinical trials ...
(Date:6/11/2019)... , ... June 10, 2019 , ... ... its distribution network with the appointment of Biofeedback Tech Ltd as ... systems are an essential part of many therapeutic treatments and clinical assessment protocols ...
(Date:6/11/2019)... ... June 10, 2019 , ... DeepDyve and ... journals to DeepDyve’s rental service for peer-reviewed journals. , IOPP’s portfolio represents ... than 20 million articles, sourced from more than 15,000 journals. , “By partnering ...
Breaking Biology Technology: