Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult)
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... The TUNEL-based assay kit provides ... control cells for convenient detection of ... tissue sections. The incorporation of BrdU ... than biotinylated or digoxigenylated methods. Results ...
The LAS-3000 imaging system combines new CCD camera technology with a simplified user interface to provide significantly improved system sensitivity with a wide range of modularities. This system is...
This kit is designed to select cells labeled with biotinylated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
This pump accomodates StemSep ; pump feed columns of the following sizes : 0.3in., 0.5in., 0.6in., and 1.0in.....
Biology Products:
(Date:11/19/2019)... DENVILLE, N.J. and HERCULES, Calif. (PRWEB) , ... ... ... provider of solutions for epigenetics research and sample preparation, and Bio-Rad Laboratories, Inc. ... diagnostic products, today announced a partnership in which Diagenode will offer Single-Cell ATAC-Seq ...
(Date:11/14/2019)... ... November 13, 2019 , ... ... Peterson, RAC , a regulatory affairs and program management executive with more than ... device industries, has joined the firm as an Expert Consultant. , Robert ...
(Date:11/12/2019)... BOWLING GREEN, Ohio (PRWEB) , ... ... ... BioFit Engineered Products , a global leader in the design and manufacturer ... comprehensive selection of seating and adjustable footrests compliant with Healthier Hospital Initiative ...
Breaking Biology News(10 mins):
(Date:1/8/2020)... ... January 08, 2020 , ... ... that can achieve specific properties for use in harsh environments inside or outside ... combines up to three metals or alloys to achieve specific properties such as ...
(Date:1/7/2020)... ... ... Vuja De Sciences (“Vuja De”), a biotechnology startup dedicated to developing cancer ... Khanna, DVM, PhD, DACVIM (Onc), and Dr. Lee Helman, MD, to its Board of ... welcome Chand and Lee as new directors. They will be of great help in ...
(Date:1/7/2020)... ... January 06, 2020 , ... ABCT , the ... ventures invited to participate in the annual program. Twelve ventures will participate in ... a professional network. All startups will receive an entrepreneur coach, access to active ...
(Date:12/30/2019)... ... December 30, 2019 , ... Leak Detection Associates (LDA), the ... Biotechnology, Medical Device and Food Packaging Industries is pleased to announce the release ... specific to the custom-built helium leak testing systems it designs and manufactures. The ...
Breaking Biology Technology: