Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... plasmid DNA preparations are prepared from ... from 1mg to multi-gram scales. Each ... needs, and glycerol stocks are maintained ... clonal selection (Clonal selection includes screening ...
... DNA preparations are prepared from customers ... 1mg to multi-gram scales. Small-scale ... preparations are made according to customer ... according to customer needs, and glycerol ...
... sports 16-independent channels for automated gigaseal patch ... screening against voltage-gated ion channels, such as ... channels. The four pipette heads afford ... ion channel drug discovery. The glass-coated ...
... The Digital Clamp One can operate ... or as a Conductance (Dynamic) Clamp in electrophysiology ... is as good as conventional analog equipment. ... recent advances in the capabilities of Digital Signal ...
Biology Products:
(Date:8/7/2018)... IRVINE, Calif. (PRWEB) , ... August 06, 2018 ... ... latest evolution of their highly successful business intelligence platform. Inspired by its medical ... streamline the entire user experience on-site. Among the many changes, SmartTRAK 3.0 touts ...
(Date:8/2/2018)... ... August 02, 2018 , ... ... in our Austin office, has been named Practice Group Chair for the Pharmaceuticals ... to help continue growing Dickinson Wright’s healthcare and life sciences initiatives, and appreciate ...
(Date:8/1/2018)... ... ... Dr. Asher Kimchi, Founder and Chairman of the International Academy of ... the 23rd World Congress on Heart Disease held in Boston, MA, USA. In addition ... Fellowship Awards. , Dr. Asher Kimchi, together with Chairman Dr. John A. Elefteriades, headed ...
Breaking Biology News(10 mins):
(Date:8/9/2018)... ... August 09, 2018 , ... MedEvolve, Inc. ... engagement and analytics software and services for physician practices, announced today the company’s ... history. After back-to-back record quarters in Q2 and Q3 last year, the company ...
(Date:8/1/2018)... ... August 01, 2018 , ... ... announces research results on the significant impact of a proprietary biofield energy ... , The preclinical research assessed biomarkers for systemic and organ-specific inflammation, as ...
(Date:7/26/2018)... ... July 26, 2018 , ... Ariadne Afaganis, an ... biofield energy treated nutraceutical to improve anti-inflammatory and immune function responses. , Biomarkers ... the preclinical research, were significantly reduced. Results are as follows:, ...
(Date:7/25/2018)... Pa. (PRWEB) , ... July 25, 2018 , ... ... technology-enabled solutions for managing clinical trial patient and supplies data through its flagship ... entitled, “Spotlight on IRT: Best Practices.” , This podcast features both Almac thought ...
Breaking Biology Technology: