Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... loading buffer is especially formulated for non-denaturing ... acids. The gel loading solution contains a ... add density and facilitate sample loading. EDTA ... require divalent cations and SDS has been ...
... Description: 100 ... Detects 70 kDa YAP from Human/mouse/rat ... immunohistochemistry. Research ... Storage: -20C ...
...
... M-PER Mammalian Protein Extraction Reagent ... lysis solution that means no ... no sonication! With M-PER Reagent, ... five minutes at room temperature, ...
Biology Products:
(Date:10/2/2018)... ... October 02, 2018 , ... ... research laboratory, ACEA Biosciences, The Cell Analysis Company , is excited to ... for immuno-oncology applications. Experimental design and data analysis for immune oncology researchers are ...
(Date:10/1/2018)... ... October 01, 2018 , ... Myoscience, ... and Chief Executive Officer. Mr. Still will also be joining the Company’s Board ... medical devices and diagnostics. He has extensive leadership experience in designing and implementing ...
(Date:10/1/2018)... ... October 01, 2018 , ... VSee, ... and InteloMed, the leading patient monitoring technology providing real-time information on individual patient ... indications of patient health during a telehealth visit without the use of traditional ...
Breaking Biology News(10 mins):
(Date:10/16/2018)... ... October 15, 2018 , ... The American Medical Informatics Association (AMIA) is ... receive their awards at the AMIA 2018 Annual Symposium , taking place Nov. ... attendees. This year garnered more than 700 submissions. The AMIA Signature Awards program provides ...
(Date:10/5/2018)... , ... October 04, 2018 , ... ... it has received a Spine Technology Award from Orthopedics This Week during the ... week. The Power T Handle is a surgical instrument that delivers operating room ...
(Date:10/2/2018)... ... October 02, 2018 , ... Genedata, ... a new out-of-the-box integration of the Genedata Bioprocess ® platform with ... equipment supplier. This enables the automatic import of raw instrument data into ...
(Date:9/28/2018)... ... 28, 2018 , ... GlycoMark®, Inc. has announced a partnership ... test, the only FDA-cleared blood test specific to detecting recent hyperglycemia and hyperglycemic ... the GlycoMark test to its network of physicians through its sales organization and ...
Breaking Biology Technology: