Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
... Error-free cloning of BAC (100+kb), fosmid ... Greater insert stability than any othe BAC ... for high yields and easy recombinant DNA ... or transcription-free (pSMART VC) vectors for the ...
... cloning of BAC (100+kb), fosmid (40kb), and ... stability than any othe BAC vector. On ... yields and easy recombinant DNA purification. Available ... (pSMART VC) vectors for the highest insert ...
Mouse Exodus 2 - 12kDa...
TGF-beta 2 MAb (Clone 8607)...
Biology Products:
(Date:7/30/2014)... is paving the way for a breakthrough that ... troops exposed to poisonous chemicalsparticularly those in pesticides ... the current issue of the journal ChemBioChem ... are compounds commonly used in pesticides and warfare ... School of Engineering Associate Professor of Chemical and ...
(Date:7/30/2014)... potential effects of climate change on the world,s ... wrong factors, according to a new paper by ... University of Queensland, and other organizations. The authors ... missing the point when it comes to climate ... change scientists focus on the "direct" threats of ...
(Date:7/30/2014)... pleased to announce its new relationship with the International ... CINP will partner to publish International Journal of ... journal. , Alan Frazer, Editor-in-Chief of IJNP ... of OUP will further facilitate the goal for the ... leading journals within neuropsychopharmacology. The conversion of the ...
Breaking Biology News(10 mins):Engineering a protein to prevent brain damage from toxic agents 2Conservation scientists asking wrong questions on climate change impacts on wildlife 2OUP to partner with the International College of Neuropsychopharmacology 2
... doesn,t stop at borders. Mexico, the U.S., and Canada ... and resource problems. In an effort to promote active ... of Geoscience and the Institute of Geology of the ... Mxico; UNAM) are hosting the 108th meeting of GSA,s ...
... (NIMML) research team at Virginia Tech has discovered important ... (CLA) in treating Crohn,s disease, a form of inflammatory ... acid found in meat and dairy products known for ... collaboration with the Division of Gastroenterology and Hepathology at ...
... MD FASEB MARC (Maximizing Access to Research Careers) ... Experimental Biology 2012 (EB 2012) meeting in San Diego, ... meant to promote the entry of underrepresented minority students, ... science community and to encourage the participation of young ...
Cached Biology News:Geological Society of America Cordilleran section meets in the heart of Mexico 2Geological Society of America Cordilleran section meets in the heart of Mexico 3Geological Society of America Cordilleran section meets in the heart of Mexico 4Researchers discover novel therapy for Crohn's disease 2MARC travel awards announced for EB 2012 2MARC travel awards announced for EB 2012 3MARC travel awards announced for EB 2012 4MARC travel awards announced for EB 2012 5
(Date:7/31/2014)... Chicago, IL (PRWEB) July 31, 2014 ... wave of automation across the U.S., increasing unemployment, ... middle class. It is a Catch 22—we ... internationally, but this means fewer jobs,” says economist ... the just-released Pollina Corporate Top 10 Pro-Business States ...
(Date:7/31/2014)... Boston’s Adult Stem Cell Technology Center, ... adult stem cell biotechnologies. Currently the company holds ... additional patent applications currently under examination by the U.S. ... two of the most vexing problems in adult stem ... cells are difficult to identify; and they have been ...
(Date:7/30/2014)... 30, 2014 W. R. Grace ... of the REVELERIS® Prep purification system , ... chromatographers, and other researchers to perform both flash ... unit. , With the REVELERIS® Prep purification system, ... chromatography modes with a simple touch of the ...
(Date:7/30/2014)... 30, 2014  Replikins Ltd. today released new data ... virus. Before the current outbreaks, the mean Ebola Reston ... acids) between 1995 and 2002 was 1.1; the mean ... and sixteen-fold in 2013, thus predicting the current outbreaks ... has been shown to be able to predict outbreaks ...
Breaking Biology Technology:ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 2ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 3ITRA Global Affiliate’s Pollina Corporate Top 10 Pro-Business States Minimize the Impact of Automation on Middle Class and Unemployment 4The Adult Stem Cell Technology Center, LLC Launches A Marketing Campaign To License Adult Stem Cell Biotechnologies 2The Adult Stem Cell Technology Center, LLC Launches A Marketing Campaign To License Adult Stem Cell Biotechnologies 3Grace Launches New REVELERIS® Prep Purification System 2