Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies
RABBIT ANTI SRC-RSV...
SHEEP ANTI FLECAINIDE...
MOUSE ANTI HUMAN B-CELLS (FMC7):FITC Immunogen: HRIK cells - Human B-Lymphoblastoid line...
... use in refrigerators, freezers, or liquid nitrogen. Conveniently sized ... for color coding to project, user, lab, etc. Holes ... cool air circulation. Supplied with 10 x 10 (11.6 ... Box dimensions: 13 x 13 x 5.1 cm H ...
Biology Products:
(Date:4/17/2014)... the Cell Press journal Current Biology on ... novel sex lives. The Brazilian insects, which represent four ... , are the first example of an animal with ... identified in several different animals, Neotrogla is ... also reversed," says Kazunori Yoshizawa from Hokkaido University in ...
(Date:4/17/2014)... are chock full of wild animals and plant life, ... fungi are churning away in the soil, decomposing organic ... the vital role these fungi play in ecological systems, ... team of scientists has generated a genetic map of ... The work was published this week in the ...
(Date:4/17/2014)... about domestication derive from Charles Darwin, whose ideas ... practices during the 19th century, a period when ... It is from Darwin that we inherit the ... from wild species and total human control over ... in this industrial setting has been applied too ...
Breaking Biology News(10 mins):In sex-reversed cave insects, females have the penises 2Stanford biologists help solve fungal mysteries 2Stanford biologists help solve fungal mysteries 3The story of animal domestication retold 2The story of animal domestication retold 3The story of animal domestication retold 4
... dependent on the sun. Sunlight does not only supply ... So-called photoreceptors activate the processes of germination, leaf development, ... component of a photoreceptor may be replaced by a ... effects on complete plants are now described in the ...
... a study about to be published in EPJ E, French ... the behaviour of a thin layer of cells in contact ... fluid or elastic films. Understanding the mechanism by which a ... the layer,s structural integrity, bears great significance because the human ...
... release is available in Spanish . , Due ... region, many experts have claimed that they are millennia old but ... explained to SINC by the ecologist Bernat Claramunt from the Centre ... from this centre has now analysed the ages of the famous ...
Cached Biology News:The millennium-old olive trees of the Iberian Peninsula are younger than expected 2
(Date:1/15/2014)... , Jan. 15, 2014  Bill Jacobs Automotive, a group of ... teaming up with the Heartland Blood Center and offering free ... The drive takes place Saturday, Jan. 18 at the Bill ... p.m. (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The ...
(Date:1/15/2014)... 2014 Cynvenio, a cancer diagnostics company ... genomic analysis of tumor cells in the bloodstream, today ... cancer decision support application powered by CollabRx, Inc. ... genomic test, Therapy Finder will provide breast cancer patients ...
(Date:1/15/2014)... 15, 2014 A study has been launched ... Formula 1 track could help to tackle the problem of ... Stowhealth (a GP surgery based in Stowmarket) and academics at ... Telemetry technology, which is inspired by equipment used ...
(Date:1/15/2014)... 15, 2014 DTS Language Services, Inc ... Web Portal for Life Science organizations who need document translations. ... subject matter of their documents in advance with a selection ... time-to-delivery of translations, often a critical factor in clinical and ...
Breaking Biology Technology:Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 2Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 3Formula 1 Technology Tackles Obesity in Unique Healthcare Partnership 4DTS Improves Efficiency for Life Science Document Translations 2