Navigation Links
Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Peripheral blood leukocyte from OriGene Technologies

ProductsRapid-Screen Arrayed cDNA Library Panels Master Plates -Human Peripheral blood leukocyte from OriGene Technologies
Company OriGene Technologies
Item Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Peripheral blood leukocyte
Price $925.00
Description The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 clones. Having identified the positive well(s) by gel electrophoresis of the reaction products, the second PCR analysis is carried out in the corresponding 96-well "Sub-Plate(s)," which contains all 5,000 clones from the positive well of the Master Plate arrayed at only 50 clones per well. Since this plate contains glycerol stocks of E. coli instead of plasmid DNA, the cells can be diluted and plated out once a positive well(s) has been identified. Analysis of 96 single colonies by PCR leads to the identification of the positive clone from the Sub-Plate well. To ensure that a clone detected in a specific well in the Master Plate will be represented in the appropriate Sub-Plate, the Sub- Plates were not derived by dilution of individual wells from the Master Plate (a process which may lose specific clones). Instead, the Sub-Plates were generated first and the wells from each Sub-Plate were then pooled to generate the Master Plate. To guarantee that clones from different wells in the Master Plate were each independently-derived, the Sub-Plates were generated with primary transformants that had not been amplified.
Info OriGene TechnologiesOriGene Technologies
6 Taft Court, Suite 100
Rockville, MD 20850
USA

Call OriGene Technologies to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-592-4179
Customer Service: 1-888-267-4436 (U.S. only) / 301-340-3188
Fax Number: 301-340-9254
Web Site: http://www.origene.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Placenta from OriGene Technologies
2. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Heart from OriGene Technologies
3. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
4. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies
10. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies
11. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies
Apoptosis Detection Systems and Reagents...
...
DAPI: 4', 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4g/mL DAPI suspended in Antifade....
Anti-Mouse Qa.m8, Ascites (Clone 5035-24.1) (mouse IgG3)...
Biology Products:
(Date:9/17/2014)... in the creation of the Santa Ana Wildfire Threat ... threat potential of the powerful, hot, dry Santa Ana ... The index was introduced Sept. 17 by the U.S. ... Gas and Electric. , The index includes four classification ... used to help fire agencies and other emergency responders, ...
(Date:9/17/2014)... doctoral student have designed a device based on a ... and dew., The device could provide water in drought-stricken ... Cheng Luo, professor in the Mechanical & Aerospace Engineering ... College of Engineering department, published "Bioinspired Plate-Based Fog Collectors" ... Society) Applied Materials & Interfaces ...
(Date:9/17/2014)... DIEGO, Calif. (Sept. 17, 2014) A rare genetic disorder ... according to a recent joint investigation by researchers at ... San Diego. In addition to suggesting better treatment options ... more clues into the genetic underpinnings of autism., Jacobsen ... the National Institutes of Health. It occurs in a ...
Breaking Biology News(10 mins):UCLA scientists play key role in developing new Santa Ana Wildfire Threat Index 2Shorebird's beak inspires UT Arlington research on water collection 2A link between Jacobsen syndrome and autism 2
... in the branches of trees hemming the shorelines during ... coastal residents in Indonesia, India, Thailand and Sri Lanka. ... Chandra Giri from the U.S. Geological Survey (USGS) decided ... which make up valuable forest ecosystems called mangroves -- ...
... molecular machines that transport substances, catalyze chemical reactions, pump ... amino acids and the individual amino acid sequence is ... protein can carry out inside the cell are determined ... this so-called tertiary structure presents a great challenge to ...
... of an exotic, infectious virus that has caused three recent ... virus might damage lungs during infection. The findings also suggest ... Not only does the infection from monkeypox virus increase ... of proteins that keep lung tissue intact and lubricated. The ...
Cached Biology News:Root of the matter: A new map shows life-saving forests' scarcity defies past estimates 2Root of the matter: A new map shows life-saving forests' scarcity defies past estimates 3Root of the matter: A new map shows life-saving forests' scarcity defies past estimates 4Root of the matter: A new map shows life-saving forests' scarcity defies past estimates 5Structural genomics accelerates protein structure determination 2Structural genomics accelerates protein structure determination 3Deadly monkeypox virus might cause disease by breaking down lung tissue 2Deadly monkeypox virus might cause disease by breaking down lung tissue 3Deadly monkeypox virus might cause disease by breaking down lung tissue 4
(Date:9/18/2014)... Sept. 18, 2014  Neogen Corporation (Nasdaq: ... a strategic agreement with Merck Animal Health to market ... The Igenity Dairy Heifer Program consists of genomic ... the genetic potential of replacement dairy heifers. The genomic ... born, and is an excellent tool in the decision-making ...
(Date:9/18/2014)... September 18, 2014 OMICS Group ... globe to participate in the ‘Open Access Week’ ... to OMICS Group Pharma Journals from ... of the fastest emerging Sciences, accelerating with a ... services across the globe. The industry is gearing ...
(Date:9/18/2014)... and diabetes prevention, could actually hasten the development of ... in a surprising way: by changing the composition and ... of bacteria residing in our intestines. These findings, the ... today in Nature . Among other things, says ... who led this research together with Prof. Eran Segal ...
(Date:9/18/2014)... California (PRWEB) September 18, 2014 Silicon-valley ... forces with Digitaliza TXT , the latest addition ... to its Clinical Research Organization (CRO) Program, Clinovo further ... “After three days of ClinCapture training, we are now ... TXT CEO Ana Pulido. “We can also rely on ...
Breaking Biology Technology:Merck Animal Health to market Neogen's dairy genomic program 2Pharma and Omics Journals to Address Global Challenges during Open Access Week 2Pharma and Omics Journals to Address Global Challenges during Open Access Week 3Gut bacteria, artificial sweeteners and glucose intolerance 2Gut bacteria, artificial sweeteners and glucose intolerance 3Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 3