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RTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits from Roche Applied Science

ProductsRTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits from Roche Applied Science
Company Roche Applied Science
Item RTS AviTag E. coli Biotinylation Kit, Plasmid; Cloning vector and in vitro protein mono-biotinylation system for use with RTS 100 and RTS 500 E. coli HY Kits
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Description Roches RTS AviTag E. coli Biotinylation Kit, Plasmid is designed for use with high-yield RTS 100 and 500 E. coli expression kits. (Cat. No. 3 186 148, 3 186 156, 3 335 461). This kit provides vectors for the easy introduction of a C- or N-terminal AviTag peptide sequence. The expressed protein can be enzymatically mono-biotinylated by E. coli biotin protein ligase (BirA, E.C. 6.3.4.15). The vectors contain all regulatory elements necessary for expression in RTS reactions using a combination of T7 RNA polymerase and prokaryotic cell lysate. In addition, the kit supplies E. coli Bir A, as well as the substrate energy mix, which are required for the biotinylation reaction.
Info Roche Applied ScienceRoche Applied Science
Roche Diagnostics Corporation
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Indianapolis, IN 46250-0414 USA




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