Navigation Links
Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsPyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek®
Price 
Description Molecular Probes EnzChek Pyrophosphate Detection Kit provides a fast, convenient and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate (PPi ) produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.1 The EnzChek Pyrophosphate Assay Kit is a modification of our EnzChek Phosphate Assay Kit (E-6646), which is based on the method originally described by Webb.2 In the presence of inorganic phosphate (Pi ), the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted enzymatically by purine nucleoside phosphorylase (PNP) to ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine (Figure 1). Enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm for the substrate to 360 nm for the product.

The EnzChek Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of PPi present into two equivalents of Pi. The Pi is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of PPi to two molecules of Pi. The assay can detect as little as 1 nanomole of PPi in 1 mL reactions (Figure 2) and can be performed over a pH range of 6.5 to 8.5.2 The MESG/PNP reaction has been used for continuous spectrophotometric determination of aminoacyl-tRNA synthetase activity.3 In addition, we have tested the ability of this assay to detect the PPi liberated by the activities of acetyl-CoA synthetase (Figure 3) and luciferase (Figure 4). In these experiments, we have detected as few as 5 10-5 U of acetyl-CoA synthetase activity and 10-2 U of luciferase activity.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
3. Pyrophosphate Colorimetric / Fluorometric (resorufin) Assay Kit, PiPer from Molecular Probes (Invitrogen)
4. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
5. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
6. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
7. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
8. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
9. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
10. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
... APAgene kits are designed to rapidly ... using our patented APA Technology. ... at an extraordinarily competitive price. All ... kit. The Locator Kit can be ...
Recommended for supporting the growth of anchorage-dependent cells including human and mouse stromal cells, mesenchymal cells and cell lines....
... This CLS number is a ... easily match Cornings product number. ... please order under the old ... customer service for assistance. ID ...
Extremely high efficiency packaging extracts useful for transfecting our EpiFOS™ or CopyControl™ Fosmids, or any DNA vector with cos sites. A tremendous value when conpared to the competition...
Biology Products:
(Date:10/31/2014)... synchrotron source that emits over a wide part of ... is currently being built in Lund, Sweden. The MAX ... the team putting together its component parts in a ... circumference of just a few hundred metres. Nevertheless, if ... new class of experiments that require source brightness and ...
(Date:10/31/2014)... steps involved in programmed cell death, offering new targets ... neurodegenerative diseases. , The research teams from the ... the three-dimensional structure of a key cell death protein ... it causes cell death. Their studies were published in ... Proceedings of the National Academy of Sciences . ...
(Date:10/30/2014)... a $1.04 million grant from the U.S. Army ... osteoarthritis using a patient,s own stem cells, spurred ... polymers., Liping Tang, a bioengineering professor and interim ... is primarily focused on helping soldiers who are ... if we inject microscaffolding that we,ve developed into ...
Breaking Biology News(10 mins):A new generation of storage -- ring 2A matter of life and death: Cell death proteins key to fighting disease 2UTA researcher uses microscaffolding injections to mend cartilage, prevent osteoarthritis 2
... available in French . , A new ... University reveals that ghrelin - a stomach hormone, acts on ... food related cues and eating for pleasure. This study, ... critical to advance understanding and treating obesity, a condition affecting ...
... There are two ways in which cooperation is the theme ... Mary Anne Alliegro, scientists at the Marine Biological Laboratorys (MBL) ... the papers acknowledgements, where the Alliegros thank those who helped ... State University (LSU) in New Orleans and their lives ...
... N.Y., May 5 bioMETRX Inc., (OTC,Bulletin Board: ... products, today announced that it has closed an ... group of previous angel,accredited investors., "We have ... team.,The company has demonstrated to us the ability ...
Cached Biology News:Hunger hormone: Makes food more attractive 2The cooperative view: New evidence suggests a symbiogenetic origin for the centrosome 2bioMETRX, Inc. Closes Angel Round of Funding 2
(Date:10/31/2014)... Following the successful launch of On ... to introduce its new product and demonstrate its potential ... Launched in September, Formedix On Demand Services is the ... automation tools. For the first time, these market-leading tools ... introducing Formations – a revolutionary new token-based payment system ...
(Date:10/31/2014)... (PRWEB) October 31, 2014 Stronger ... healthcare, construction, finance and consumer electronics will help ... reason, industry research firm IBISWorld has added a ... its growing industry report collection. , The Biometrics ... for biometric technologies such as fingerprint, iris, retina ...
(Date:10/30/2014)...  Ardelyx, Inc. (NASDAQ: ARDX ), ... and metabolic diseases, today announced that it will ... November 6, 2014. Following the announcement, the Ardelyx ... and webcast at 4:30pm ET to review the ... The live webcast can be accessed ...
(Date:10/30/2014)... (PRWEB) October 30, 2014 Rancho ... that they have manually curated public data source ... profit customers. the data is now available for ... and unifies public and internal data sources for their ... knowledge out of the data that becomes more easily ...
Breaking Biology Technology:Formedix to Introduce "Revolutionary" Clinical Trial Automation Software with Learning Webinar 2Biometrics Scan Software in the US Industry Market Research Report Now Available from IBISWorld 2Biometrics Scan Software in the US Industry Market Research Report Now Available from IBISWorld 3Ardelyx to Report Third Quarter 2014 Financial Results on November 6, 2014 2Rancho BioSciences Manually Curated TCGA Data Sets Available 2