Navigation Links
ProtoArray Yeast PPI Kit from Invitrogen

ProductsProtoArray Yeast PPI Kit from Invitrogen
Company Invitrogen
Item ProtoArray Yeast PPI Kit
Price 
Description The Yeast ProtoArray high-density functional protein microarray is the first microarray product that enables rapid elucidation of proteinprotein interactions on a proteome scale. The Yeast ProtoArray contains 4,088 S. cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions, purified and spotted in duplicate on a 1 inch x 3 inch nitrocellulose-coated slide. With the Yeast ProtoArray PPI Kit, you can identify novel protein interactions in a single day, instead of the several weeks required using alternatives such as yeast two-hybrid systems. In addition, protein interactions can be easily assayed under different conditions to better understand interaction dynamics.


Using the Yeast ProtoArray PPI Kit, protein-protein interactions screening takes four simple steps:
Prepare your protein of interest using the included ProtoArray biotinylation reagents. • Probe the Yeast ProtoArray PPI microrray using the biotinylated protein of interest • Detect positive interactions using streptavidin-conjugated Alexa Fluor 647. • Dry and scan with most standard microarray readers.


Contents:
The complete kit contains two ProtoArray Yeast Proteome and two Control Microarrays, ProtoArray Buffer Module A, ProtoArray Buffer Module B, Mini-Biotinylation Module kit, Biotinylation Purification Module, Biotinylation Assessment Module, 30 l (2 mg/ml) streptavidinconjugate Alexa Fluor(r) 647, and 40 l (0.5 mg/ml) biotinylated calmodulin kinase. The ProtoArray Buffer Module A contains 30 ml of 30% BSA, 400 l 1M DTT. The ProtoArray Buffer Module B contains blocking and probing buffers, 4 Hybrislips coverslips, 2 hybridization chambers. The ProtoArray Mini-Biotinylation Module Kit contains 100 g of Biotin-XX, sulfosuccinimidyl ester, sodium salt, 50 g SA, and 20 ng biotinylated BSA. The ProtoArray Biotinylation Purification Module contains the purification resin in storage buffer, 4 spin columns, and 8 collection tubes. The ProtoArray Biotinylation Assessment Module contains 20 l Streptavidin AP, and 5 ml AP substrate and buffers.
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

Call Invitrogen to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-866-521-4270
Customer Service: 800-955-6288
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ProtoArray Kinase Substrate Identification Application Kit from Invitrogen
2. Yeast Nitrogen Base from Invitrogen
3. Yeast Nitrogen Base from Invitrogen
4. pYD1 Yeast Display Vector from Invitrogen
5. Yeast Protein Kit from Zymo Research
6. Yeast-GFP 96-well Plates from Invitrogen
7. Yeast-GFP Collection from Invitrogen
8. Yeast Oligo Microarray Kit (V2), 2 x 11K from Agilent Technologies
9. Frozen-EZ Yeast Transformation II Kit from Zymo Research
10. Hybrid Hunter Yeast Two-Hybrid System from Invitrogen
11. Yeast Nitrogen Base from Invitrogen
... a compact benchtop and stackable multi-function ... with a large display screen to ... Air circulation inside to evenly distribute ... be easily calibrated from the microprocessor ...
... specifically designed as a high tech shaker ... mass compensation allows high shaking speed even ... and, therefore, faster growth and higher cell ... when high reproducibility is critical ...
... --- a cocktail of 3 siRNAs specifically ... our most popular product. The siTrio reagent ... of your target gene when used under ... and confirmation of optimal transfection with one ...
... The iCycler thermal cycler with 96 x ... nucleic acid amplification (PCR) applications. It is ... (15-100 microliter samples) that is compatible with ... temperature monitoring and control by algorithm, block, ...
Biology Products:
(Date:6/9/2016)... attendance control systems is proud to announce the introduction of fingerprint attendance control software, ... employees are actually signing in, and to even control the opening of doors. ... ... ... Photo - http://photos.prnewswire.com/prnh/20160609/377487 ...
(Date:6/3/2016)... 2016 Das DOTM ... Nepal hat ein 44 Millionen ... Kennzeichen, einschließlich Personalisierung, Registrierung und IT-Infrastruktur, an ... und Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte internationale ... teilgenommen, aber Decatur wurde als konformste und ...
(Date:6/2/2016)... June 2, 2016 Perimeter Surveillance ... Unmanned Systems, Physical Infrastructure, Support & Other Service  ... visiongain offers comprehensive analysis of the global ... will generate revenues of $17.98 billion in 2016. ... Inc, a leader in software and hardware technologies for ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... ... and Mold) microbial test has received AOAC Research Institute approval 061601. , “This ... introduced last year,” stated Bob Salter, Vice President of Regulatory and Industrial Affairs. ...
(Date:6/23/2016)... , ... June 23, 2016 , ... ... announced the launch of the Supplyframe Design Lab . Located in Pasadena, ... explore the future of how hardware projects are designed, built and brought to ...
(Date:6/23/2016)... Calif. , June 23, 2016  Blueprint Bio, ... biological discoveries to the medical community, has closed its ... Matthew Nunez . "We have received ... with the capital we need to meet our current ... essentially provide us the runway to complete validation on ...
(Date:6/23/2016)... ... June 23, 2016 , ... Regulatory Compliance Associates® Inc. (RCA), ... free webinar on Performing Quality Investigations: Getting to Root Cause. ... no charge. , Incomplete investigations are still a major concern to the Regulatory ...
Breaking Biology Technology: