Navigation Links
ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.

ProductsProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent
Price 
Description ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent

A powerful new method known as Label Transfer has rapidly gained traction as an in vitro method for protein interaction discovery. A growing number of publications feature the exclusive Pierce ProFound Sulfo-SBED Biotin Label Transfer Reagent to study a variety of issues relating to protein interactions. • The use of Sulfo-SBED has contributed greatly to published studies on protein:protein interactions. To simplify the method, Pierce has assembled the key components frequently cited in publications featuring Sulfo-SBED in the label transfer application. The resulting easy-to-use kit also contains Streptavidin-HRP for subsequent detection of the biotinylated complex or biotinylated prey protein on a Western blot. •

Label transfer is an in vitro method for discovering or verifying an interaction that was observed by way of another technique such as the in vivo yeast 2-hybrid method or by in vitro methods such as co-immunoprecipitation or pull-down assays. • The label transfer method requires a purified "bait" protein. The amine groups of the bait protein are modified via the Sulfo-NHS moiety of Sulfo-SBED. This derivatized bait protein is then incubated in the dark with a lysate or with a purified putative "prey" protein. The bait and prey complex, if formed, is then captured or trapped by exposing the sample to high-intensity UV light, which activates the phenylazide moiety of Sulfo-SBED. This photoreactive group covalently links to the bound prey protein, capturing the interacting complex. Upon reduction of this complex, the biotin label that first resided with the bait protein transfers to the prey protein. The biotin label also functions as the detection target for the bait:prey complex or the prey protein upon Western blot analysis. The resulting biotin derivatized complex or prey protein can be detected using streptavidin-HRP or an anti-biotin antibody and chemiluminescent detection. The biotin label can also be used as a handle to purify the bait:prey complex over an avidin/streptavidin/NeutrAvidin/monomeric avidin biotin-binding protein support.

  • Amine group-specific reactivity
  • Nonspecific photoreactivity
  • Thiol-cleavable disulfide (S-S) linkage
  • A Biotin handle: This biotin transfers from a labeled purified bait protein to a captured prey protein (Figure 2), which makes it especially useful for Western blot detection
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Label IT Tracker CX-Rhodamine Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
2. Label IT Tracker Cy5 Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
3. Label IT Tracker BiotinIntracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
4. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
5. Label IT siRNA Tracker CX-Rhodamine Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
6. Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
7. Label IT siRNA Tracker Intracellular Localization Kit with TransIT-TKO Transfection Reagent (Cy5 Labeling Kit) from Mirus Bio Corporation
8. Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
9. Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
10. Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
11. Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
... EKMax™ is a recombinant preparation of ... EKMax™ Enterokinase is expressed and isolated from ... highly purified enzyme preparation (2). It recognizes ... bond after the lysine residue. The enzyme ...
... are cytopathic effect inhibition assays, also known as ... tissue culture supernatents; serum from PK studies and ... is defined as the quantity of interferon required ... viral infection by 50%. All standards ...
...
...
Biology Products:
(Date:9/2/2014)... over the last decade in Taiyuan, China, have substantially ... a greater than 50% reduction in costs associated with ... according to researchers at the Columbia Center for Children,s ... Health, the Shanxi Medical University, the Center of Diseases ... University School of Public Health. , The study is ...
(Date:9/2/2014)... take stress in stride; others are done in by ... molecular mechanisms of this so-called stress gap in mice ... could lead researchers to better understand the development of ... people, each animal has unique experiences as it goes ... experiences can alter the expression of genes, and as ...
(Date:9/1/2014)... held that corals whose calcium-carbonate skeletons form ... organisms that rely entirely on ocean currents to ... But now scientists at MIT and the Weizmann ... that they are far from passive, engineering their ... greatly enhance their ability to exchange nutrients and ...
Breaking Biology News(10 mins):Clean air halves health costs in Chinese city 2Discovery hints at why stress is more devastating for some 2Discovery hints at why stress is more devastating for some 3Nature's tiny engineers 2Nature's tiny engineers 3
... will rewrite the textbooks on how an ovary is formed, ... The study, now published in the journal PLOS ONE ... key role in the development of ovaries and ovarian follicles, which ... discovery is expected to prompt further studies around the world to ...
... low temperatures and NMR spectroscopy, the scientists visualized seven ... down from 25C to -16C. Their results show that ... in protein folding. The scientists, findings may contribute to ... and misfold during illness. Whether Alzheimer,s, Parkinson,s ...
... Researchers from North Carolina State University have found that one ... States the Argentine ant appears to have met ... have found that the Asian needle ant is successfully displacing ... needle ant with its venomous sting may be ...
Cached Biology News:Study prompts rethink of how ovaries develop 2Protein 'filmed' while unfolding at atomic resolution 2Protein 'filmed' while unfolding at atomic resolution 3Researchers find Asian needle ants displacing other aggressive invaders 2
(Date:9/2/2014)... September 02, 2014 The inventors of ... for chronic tinnitus are seeking support for development costs ... Indiegogo platform ( http://igg.me/at/tinaway ), was launched on September ... therapeutic ‘mHealth’ app and is based on ten years ... team of biomedical and software engineers, Dr. Ian Dixon ...
(Date:8/31/2014)... 2014 Available spectra is similar ... research and cultivation of a wide range of ... LEDs with proven patented and pending wide spectrum ... uniformity in critical applications in contrast to growth ... “Our customers have a large variety of differently ...
(Date:8/29/2014)... but one of the most promising technologies for future ... light (photons) instead of electrons. First, it is necessary ... photons and control their direction. Researchers around the world ... control, but now scientists at the Niels Bohr Institute ... emitted one at a time and in a particular ...
(Date:8/29/2014)... Hills, NY (PRWEB) August 29, 2014 ... Burnt Hills Veterinary Hospital are seeking candidates to participate ... dogs with osteoarthritis. The ultimate goal of this ... donor stem cells into one or two arthritically affected ... treated joints. , Candidates for the current investigational ...
Breaking Biology Technology:tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 2tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 3tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 4tinAway Pty. Ltd. launch a crowdfunding campaign to fund a clinical trial of tinAway, a new potential cure for chronic tinnitus 5Breakthrough in light sources for new quantum technology 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2