Navigation Links
ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.

ProductsProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent
Price 
Description ProFound Label Transfer Sulfo SBED Protein:Protein Interaction Reagent

A powerful new method known as Label Transfer has rapidly gained traction as an in vitro method for protein interaction discovery. A growing number of publications feature the exclusive Pierce ProFound Sulfo-SBED Biotin Label Transfer Reagent to study a variety of issues relating to protein interactions. • The use of Sulfo-SBED has contributed greatly to published studies on protein:protein interactions. To simplify the method, Pierce has assembled the key components frequently cited in publications featuring Sulfo-SBED in the label transfer application. The resulting easy-to-use kit also contains Streptavidin-HRP for subsequent detection of the biotinylated complex or biotinylated prey protein on a Western blot. •

Label transfer is an in vitro method for discovering or verifying an interaction that was observed by way of another technique such as the in vivo yeast 2-hybrid method or by in vitro methods such as co-immunoprecipitation or pull-down assays. • The label transfer method requires a purified "bait" protein. The amine groups of the bait protein are modified via the Sulfo-NHS moiety of Sulfo-SBED. This derivatized bait protein is then incubated in the dark with a lysate or with a purified putative "prey" protein. The bait and prey complex, if formed, is then captured or trapped by exposing the sample to high-intensity UV light, which activates the phenylazide moiety of Sulfo-SBED. This photoreactive group covalently links to the bound prey protein, capturing the interacting complex. Upon reduction of this complex, the biotin label that first resided with the bait protein transfers to the prey protein. The biotin label also functions as the detection target for the bait:prey complex or the prey protein upon Western blot analysis. The resulting biotin derivatized complex or prey protein can be detected using streptavidin-HRP or an anti-biotin antibody and chemiluminescent detection. The biotin label can also be used as a handle to purify the bait:prey complex over an avidin/streptavidin/NeutrAvidin/monomeric avidin biotin-binding protein support.

  • Amine group-specific reactivity
  • Nonspecific photoreactivity
  • Thiol-cleavable disulfide (S-S) linkage
  • A Biotin handle: This biotin transfers from a labeled purified bait protein to a captured prey protein (Figure 2), which makes it especially useful for Western blot detection
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Label IT Tracker CX-Rhodamine Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
2. Label IT Tracker Cy5 Intracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
3. Label IT Tracker BiotinIntracellular Nucleic Acid Localization Kit from Mirus Bio Corporation
4. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
5. Label IT siRNA Tracker CX-Rhodamine Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
6. Label IT siRNA Tracker CX Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
7. Label IT siRNA Tracker Intracellular Localization Kit with TransIT-TKO Transfection Reagent (Cy5 Labeling Kit) from Mirus Bio Corporation
8. Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation
9. Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
10. Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
11. Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation
Because it is manufactured at our ISO 9001–certified facilities in Eugene, Oregon, we can guarantee that FluoroPure Grade DAPI is greater than or equal to 98% pure by HPLC....
Edited by D.M. Gersten (1996) Provides comprehensive descriptions of the techniques available for analytical gel electrophoresis of proteins and offers clear step-by-step protocols for all of the m...
See product name for description....
Whole blood is collected from healthy pigs (mixed breed, either sex) at the time of slaughter, refrigerated within 6 hours, processed within 24 hours and frozen at -20ºC....
Biology Products:
(Date:10/30/2014)... is available in German . ... prompt our fellow humans to behave socially? This is ... in game theory. Previous studies assumed that it is ... scientists from the Max Planck Institute for Evolutionary Biology ... an influence on the cooperative behaviour of others. However, ...
(Date:10/30/2014)... , Oct. 29, 2014  Securus Technologies, ... justice technology solutions for public safety, investigation, ... has deployed exciting enhancements to its THREADS™ ... to provide actionable intelligence and focused leads ... to drive technological innovation through identifying and ...
(Date:10/29/2014)... for the Society of Interventional Radiology,s (SIR) ... the one place where interventional radiologists, diagnostic ... professionals can come together to find the ... presented, discussed and tested. , "Connecting ... medicine, SIR 2015 will feature essential updates ...
Breaking Biology News(10 mins):Together we are strong -- or insufferable 2Together we are strong -- or insufferable 3Together we are strong -- or insufferable 4Securus Technologies Adds Advanced Features to Its Industry-Leading Investigative Analytics Product 2SIR 2015: Interventional radiology shapes tomorrow's medicine 2
... American Botanical Council (ABC) has just published a ... Sinupret, a clinically-tested herbal combination for upper respiratory ... bronchitis. A monograph is a technical paper focusing ... in the monograph include four clinical trials regarding ...
... Seawater is a complex, dynamic mixture of dissolved ... best efforts, presents difficulties in measuring its potential ... itself, the calculations scientists employ to measure seawater ... the years as research techniques and instrumentation continues ...
... Carnegie,s Department of Embryology, has been elected one ... Microbiology. Fellows are annually elected "through a highly ... scientific achievement and original contributions that have advanced ... Saccharomyces cerevisiae , Koshland has become a leader ...
Cached Biology News:ABC publishes monograph on scientific and clinical research of Sinupret 2ABC publishes monograph on scientific and clinical research of Sinupret 3ABC publishes monograph on scientific and clinical research of Sinupret 4New equation of state of seawater 2New equation of state of seawater 3Carnegie's Doug Koshland elected Fellow of the American Academy of Microbiology 2
(Date:10/30/2014)... Global polyvinyl chloride (PVC) demand registered ... during 2008-2009, especially in North America and Europe. However, ... keeping to a growing trend. , The largest chunk ... the construction segment. In 2013, the key PVC application ... and rigid film. Moreover, the PVC was consumed in ...
(Date:10/30/2014)... SoundConnect , an industry leading ... end users the key to turning this already ... adding voice enablement and hybrid audio conferencing. , ... communication tool that includes presence, instant messaging and ... becomes a comprehensive unified communications solution. Adding the ...
(Date:10/30/2014)... 2014 Spartan Bioscience announced today that ... System . It detects CYP2C19 genetic mutations in less ... The Spartan RX CYP2C19 System is the first near-patient ... in Canada. Due to the system’s ease of use, ... by healthcare professionals such as doctors, nurses, pharmacists, and ...
(Date:10/30/2014)... Ras Al Khaimah, UAE (PRWEB) October 30, 2014 ... the appointment of Dr. James A. Manganello, Chairman of the ... (NSCEC) of The Bahamas. The appointment was made by ... The Bahamas on September 24th, 2014. , More ... Cell Therapy and Research Bill , Prime Minister Perry Christie ...
Breaking Biology Technology:Global Polyvinyl Chloride Demand Is on Upward Trend Now, Says Merchant Research & Consulting in Its Topical Study 2Global Polyvinyl Chloride Demand Is on Upward Trend Now, Says Merchant Research & Consulting in Its Topical Study 3SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 3Health Canada approves first near-patient DNA test for personalized medicine 2Health Canada approves first near-patient DNA test for personalized medicine 3Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3