|Products||Phosphate Sensor from Invitrogen|
|Description|| Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate. This product is a purified form of recombinant E. coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment.How it worksBinding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ~ 0.1 M), resulting in a large increase in fluorescence. Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases. Likewise, Phosphate Sensor can be used to detect and characterize inhibitors of phosphate-producing enzymes. Furthermore, unlike malachite green, Phosphate Sensor can be used to monitor phosphate formation in real time. Contents and Storage:Phosphate Sensor is supplied as a 500 M solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80C.|
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