|Products||PR Competitor Assay Green from Invitrogen|
|Item||PR Competitor Assay Green|
|Description|| Progesterone Receptor (PR) Competitor Assay Kits are ideal for studying or screening novel progesterone receptor binding compounds using fluorescence polarization (FP) The homogenous assay utilizes a fusion of glutathione transferase to the ligand binding domain of human progesterone receptor [PR-LBD(GST)] and a proprietary, fluorescently-tagged progesterone ligand, Fluormone PL Green or PL Red. Intrinsic background fluorescence from library compounds is substantially reduced utilizing the red-shifted Fluormone ligand.|
To assay: PR added to Fluormone L Green or PL Red complexes and has a high polarization value. Test compounds that compete for binding to PR and prevent the formation of a Fluormone/PR complex will result in a lower polarization value. The shift in polarization in the presence of test compounds is used to determine the relative affinity of test compounds for PR.
The Progesterone Receptor Competitor Assay Kits contain partially purified PR protein, Fluormone PL Green (green assay kit) or PL Red (red assay kit) proprietary fluorescent progesterone ligands, and buffer.
1600 Faraday Ave.
Carlsbad, CA 92008
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com