Navigation Links
Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems

ProductsNeural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
Company R&D Systems
Item Neural Stem Cell Expansion Kit - Monolayer Plus
Price $335.00
Description The Neural Stem Cell Expansion Kit - Monolayer Plus is a system designed for in vitro NSC expansion as a monolayer in a serum-free environment. The kit contains a specially formulated N-2 Plus Media Supplement, which has been optimized for neural stem cell expansion (Johe, K.K. et al. (1996) Genes & Development 10:3129). Human fibroblast growth factor basic (FGF basic) is included to promote the in vitro proliferation of neural precursors. Human epidermal growth factor (EGF) is also included and may be used for optimizing human neural precursor growth in culture. However, the use of EGF is optional, and its effect, other than stimulating cell growth, is still under investigation. Bovine Fibronectin, used to coat plates to promote cell attachment and spreading, is also included. The quantity of each component provided in the kit is estimated to be sufficient to make 500 mL of growth medium for cell expansion. This kit is suitable for the monolayer culture model and is applicable for human, rat and mouse cells. Cells propagated using this kit are Nestin-positive (Lendahl, U. et al. (1990) Cell 60:585) and retain their potency to differentiate into neurons, oligodendrocytes and glia, as identified after growth factor withdrawal by staining with the antibodies Tuj1, O4 and anti-GFAP, respectively.

Neural stem cells (NSCs), with the potential to self-renew and to differentiate into neurons and glial cells, have been isolated from different regions of the developing and adult brain (1 - 6). NSCs can be grown in vitro, in the presence of mitogen, as either monolayer cultures on an adhesive substrate or as free floating spherical aggregates termed 'neurospheres. These in vitro propagated NSCs can proliferate in culture while retaining the potency to differentiate into neurons, oligodendrocytes and glia. in vitro NSC expansion makes it possible to grow sufficient numbers of NSCs for researchers to study basic developmental biology, drug discovery and preclinical neural transplantation.

  1. Alvarez-Buylla, A. et al. (1998) J. Neurobiol. 36:105.
  2. McKay, R.D. (1997) Science 276:66.
  3. Reynolds, B.A. et al. (1992) Science 255:1707.
  4. Johansson, C.B. et al. (1999) Cell 96:25.
  5. Kilpatrick, T.J. et al. (1993) Neuron 10:255.
  6. Davis, A.A. et al. (1994) Nature 372:263.

Neural Stem Cell Expansion Kits SC002 & SC005 are suitable for a monolayer culture model, and Neural Stem Cell Expansion Kit SC003 is for a neurosphere culture model.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
2. Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
7. Expansion and Cryopreservation of Cells from Meridian Life Science, Inc.
8. Stem Cell Expansion Media 1 from 3H Biomedical AB
9. Mouse Hematopoietic Stem Cell Expansion Cytokine Panel from R&D Systems
10. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
11. Free Activin A ELISA Kit from Diagnostic Systems Laboratories, Inc.
Nuclear/Cytosol Fractionation Kit Research Focus: other Storage Temperature: 4C Shipping Temperature: 4C...
Cytosol/Particulate Rapid Separation Kit Research Focus: other Storage Temperature: -20C Shipping Temperature: 4C...
Request Info...
Purified anti-human CD182 (CXCR2)...
Biology Products:
(Date:4/14/2015)... , April 14, 2015  HYPR Corp. ... Online (FIDO ® ) Alliance tm , an ... FIDO members commit to share technology and collaborate ... that are interoperable, more secure and private, and ... biometric identity verification that protects sensitive user information ...
(Date:4/9/2015)... April 9, 2015 Synaptics Inc. (NASDAQ: SYNA ... today that it will report financial results for the ... 2015, after the close of market. The company will ... at 2:00 p.m. PT (5:00 p.m. ET), during which ... participate on the live call, analysts and investors should ...
(Date:4/2/2015)... Sweden , April 2, 2015 ... for Q1 2015 attributed to somewhat higher than 125 ... in the year-end report 2014 that revenues for Q1 ... that the company reported for Q4 2014. The operating ... to be negative. The complete interim report will as ...
Breaking Biology News(10 mins):HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Synaptics to Report Third Quarter Results on April 23 2Fingerprint Cards Revises Revenues for Q1 2015 2
... the first researchers to study the deadly link between ... Association lifetime achievement award today at a ceremony in ... endocrinologist James Sowers, MD, recognizes his more than 30 ... The Irvine Page-Alva Bradley Lifetime Achievement Award in ...
... it they,re very good at what they do. But ... out inspection work in oily or dangerous environments, or in ... they,re one of the fastest and most maneuverable creatures on ... speeds due to their streamlined bodies and a finely tuned ...
... , New Rochelle, NY, September 19, 2012 Many ... will largely be based on the field of ... News ( GEN ) ( http://genengnews.com ). ... larger arrays of biosamples, demand is exceeding supply, ...
Cached Biology News:Pioneering researcher receives Heart Association Lifetime Achievement Award 2Pioneering researcher receives Heart Association Lifetime Achievement Award 3Robotic tuna is built by Homeland Security 2
(Date:4/17/2015)... 2015 /PRNewswire/ - SQI Diagnostics Inc. (TSX-V: SQD; OTCQX: ... develops and commercializes proprietary technologies and products for ... has received approval from the TSX Venture Exchange ... share purchase warrants ("the Warrants") of the Company, ... May 2013 private placement financing. Each ...
(Date:4/17/2015)... 17, 2015  US-Australian drug discovery company, Novogen, today ... Understanding with the Feinstein Institute for Medical Research ... to collaborate with the objective of developing ... collaboration brings together the drug discovery expertise of ... the Feinstein Institute in neurosciences and oncology. ...
(Date:4/17/2015)... 2015 LC Sciences LLC, a ... today that it is seeking beta testers for ... PCR based targeted sequencing technology. The system offers ... enables accurate detection of rare variants. It utilizes ... The company will collaborate with beta testers to ...
(Date:4/16/2015)... Spirax Sarco, the leader in ... newly designed, state-of-the-art website in the first quarter ... promotes the Spirax Sarco brand, company capabilities, and ... a site visitor might be looking for including ... serviced, the latest company news, careers, and much ...
Breaking Biology Technology:SQI Diagnostics to Extend Warrant Expiry 2SQI Diagnostics to Extend Warrant Expiry 3Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 2Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 3Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 4Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 5Novogen and Feinstein Institute Join Forces to Find Effective Treatments for Brain Cancer 6LC Sciences Seeks Beta Testers of Targeted Sequencing Method Ahead of Q4 Launch 2LC Sciences Seeks Beta Testers of Targeted Sequencing Method Ahead of Q4 Launch 3Spirax Sarco website has a new look 2