Navigation Links
Neural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems

ProductsNeural Stem Cell Expansion Kit - Monolayer Plus from R&D Systems
Company R&D Systems
Item Neural Stem Cell Expansion Kit - Monolayer Plus
Price $335.00
Description The Neural Stem Cell Expansion Kit - Monolayer Plus is a system designed for in vitro NSC expansion as a monolayer in a serum-free environment. The kit contains a specially formulated N-2 Plus Media Supplement, which has been optimized for neural stem cell expansion (Johe, K.K. et al. (1996) Genes & Development 10:3129). Human fibroblast growth factor basic (FGF basic) is included to promote the in vitro proliferation of neural precursors. Human epidermal growth factor (EGF) is also included and may be used for optimizing human neural precursor growth in culture. However, the use of EGF is optional, and its effect, other than stimulating cell growth, is still under investigation. Bovine Fibronectin, used to coat plates to promote cell attachment and spreading, is also included. The quantity of each component provided in the kit is estimated to be sufficient to make 500 mL of growth medium for cell expansion. This kit is suitable for the monolayer culture model and is applicable for human, rat and mouse cells. Cells propagated using this kit are Nestin-positive (Lendahl, U. et al. (1990) Cell 60:585) and retain their potency to differentiate into neurons, oligodendrocytes and glia, as identified after growth factor withdrawal by staining with the antibodies Tuj1, O4 and anti-GFAP, respectively.

Neural stem cells (NSCs), with the potential to self-renew and to differentiate into neurons and glial cells, have been isolated from different regions of the developing and adult brain (1 - 6). NSCs can be grown in vitro, in the presence of mitogen, as either monolayer cultures on an adhesive substrate or as free floating spherical aggregates termed 'neurospheres. These in vitro propagated NSCs can proliferate in culture while retaining the potency to differentiate into neurons, oligodendrocytes and glia. in vitro NSC expansion makes it possible to grow sufficient numbers of NSCs for researchers to study basic developmental biology, drug discovery and preclinical neural transplantation.

  1. Alvarez-Buylla, A. et al. (1998) J. Neurobiol. 36:105.
  2. McKay, R.D. (1997) Science 276:66.
  3. Reynolds, B.A. et al. (1992) Science 255:1707.
  4. Johansson, C.B. et al. (1999) Cell 96:25.
  5. Kilpatrick, T.J. et al. (1993) Neuron 10:255.
  6. Davis, A.A. et al. (1994) Nature 372:263.

Neural Stem Cell Expansion Kits SC002 & SC005 are suitable for a monolayer culture model, and Neural Stem Cell Expansion Kit SC003 is for a neurosphere culture model.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. NeuroCyte - Rat Neural Stem Cell Kit from Orion BioSolutions, Inc.
2. Neural Stem Cell Expansion Kit - Neurosphere from R&D Systems
3. Neural Stem Cell Expansion Kit - Monolayer from R&D Systems
4. Mouse/Rat Neural Stem Cell Functional Identification Kit from R&D Systems
5. Human Neural Stem Cell Functional Identification Kit from R&D Systems
6. rNeural Stem Cell (rNSC)Kit from AlphaGenix, Inc.
7. Expansion and Cryopreservation of Cells from Meridian Life Science, Inc.
8. Stem Cell Expansion Media 1 from 3H Biomedical AB
9. Mouse Hematopoietic Stem Cell Expansion Cytokine Panel from R&D Systems
10. Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
11. Free Activin A ELISA Kit from Diagnostic Systems Laboratories, Inc.
... Pierce offers maleimide activated carrier proteins ... conjugates. Imject Maleimide Activated Carrier Proteins ... been preactivated with a heterobifunctional cross-linker ... stable, sulfhydryl-reactive carrier protein. Thus ...
Cell Culture Flask, 25 cm, tissue-culture treated polystyrene...
Cell Culture Flask, nontreated polystyrene...
iNOS/NOS Type II Purified Anti-Mouse, Anti-Rat, Anti-Human clone Polyclonal, Isotype Rabbit Ig, 150 µg Consult technical datasheet for details....
Biology Products:
(Date:3/14/2016)... , March 14, 2016 NXTD ) ... mobile commerce market, announces the airing of a new series ... the week of March 21 st .  The commercials will ... its popular Squawk on the Street show. --> ... the growing mobile commerce market, announces the airing of a ...
(Date:3/10/2016)... PUNE, India , March 10, 2016 ... to a new market research report "Identity and Access ... SSO, & Audit, Compliance, and Governance), by Organization Size, ... Forecast to 2020", published by MarketsandMarkets, The market is ... to USD 12.78 Billion by 2020, at a Compound ...
(Date:3/9/2016)... , March 9, 2016  Crossmatch ® ... and enrollment solutions, today announced the addition of ... Altus multi-factor authentication platform. New contextual ... managers to step-up security where it,s needed most ... Washington, DC . --> ...
Breaking Biology News(10 mins):
(Date:4/29/2016)... 2016 According to a ... "Separation Systems for Commercial Biotechnology Market - Global ... 2015 - 2023", the separation systems for commercial ... in 2014 and is projected to expand at ... to reach US$ 19,227.8 Mn in 2023. ...
(Date:4/28/2016)... April 28, 2016 The report ... Brayton Cryocoolers), Service (Technical Support, Product Repairs & Refurbishment, ... Global Forecast to 2022", published by MarketsandMarkets, the global ... by 2022, at a CAGR of 7.29% between 2016 ... Tables and 94 Figures spread through 159 Pages and ...
(Date:4/28/2016)... ... April 28, 2016 , ... Morris ... open house for regional manufacturers at its Maple Grove, Minnesota technical center, May ... Group, Chiron and Trumpf. Almost 20 leading suppliers of tooling, accessories, software ...
(Date:4/27/2016)... April 27, 2016 NanoStruck ... (OTCPink: NSKQB) ( Frankfurt : 8NSK) ... Pressemitteilung vom 13. August 2015 die Genehmigung von ... um zusätzliche 200.000.000 Einheiten auf 400.000.000 Einheiten zu ... bringen. Davon wurden 157.900.000 Einheiten mit dem ersten ...
Breaking Biology Technology: