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NADPH-cytochrome c reductase Colorimetric Assay Kit from Sigma-Aldrich

ProductsNADPH-cytochrome c reductase Colorimetric Assay Kit from Sigma-Aldrich
Company Sigma-Aldrich
Item NADPH-cytochrome c reductase Colorimetric Assay Kit
Price 
Description Analysis Note: The kit contains all the reagents required for a fast and convenient determination of NADPH-cytochrome c reductase activity in cell and tissue extracts and in purified microsomes [endoplastic reticulum (ER)]. It has been tested on samples prepared from mammalian tissues such as liver, kidney, brain, spleen, and heart muscle from several species, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.
Biochem/physiol Actions: Eukarytic NADPH-Cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.
Features and Benefits: The kit is suitable for:

  • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation

  • Assaying NADPH-cytochrome c reductase levels in various sources after intoxication by drugs or challenge by xenobiotics

Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

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IC50: 35 ng/ml · Limit of detection: 8 ng/ml...
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Deletions, insertions or substitutions. Mutations in plasmids of size up to 15 kb. Single or multiple mutations...
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