Navigation Links
Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems

ProductsMouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
Company R&D Systems
Item Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation
Price $1,000.00
Description Reagents for the differentiation of mouse embryonic stem cells to dopaminergic neurons.

Dopamine cell bodies exist in the midbrain, where they are mainly located in the substantia nigra, the ventral tegmental area, and the retrorubral field. These dopaminergic (DA) neurons give rise to extensive forebrain dopamine innervation ( e.g.projections to the caudate nucleus, putamen nucleus accumbens, olfactory tubercle, and several cortical regions and limbic regions, including the amygdala, lateral septum and ventral hippocampus). The basic organization of midbrain dopamine neurons and their projections is consistent across most mammalians. The midbrain dopamine projections to the striatum have been among the most extensively studied catecholamine neurons, in part because the degeneration of DA neurons in the substantia nigra results in Parkinsons disease. Both animal models and clinical trials have suggested that cell replacement therapies may be effective in treating Parkinsons disease. This approach is limited, however, by the availability of a rich, effective source of neural precursors for DA neuron generation.

Embryonic stem (ES) cells, derived from the inner cell mass of pre-implantation embryos, have been recognized as the most pluripotent stem cell population. ES cells give rise to a collection of differentiated cells and tissues, including neurons. Thus, they may serve as a potential source of replacement DA neurons in Parkinsons disease. Two groups have reported methods for generating DA neurons from ES cells, thus demonstrating the feasibility of identifying conditions required for functional DA neuron differentiation of ES cells. Experimental models established from these studies might facilitate the in vitroexamination of various aspects of neural development, in particular the underlying mechanisms controlling cell lineage and fate commitment.


The Mouse Dopaminergic Neuron Differentiation Kit is a system designed for in vitroneural differentiation of mouse ES cells in a serum-free environment. This kit contains specially formulated ITS and N-2 Plus Media Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to provide proper support for cell attachment and spreading. A growth factor panel, consisting of human fibroblast growth factor (FGF) basic, mouse FGF-8b, and mouse sonic hedgehog amino-terminal peptide (Shh-N), is included for effective DA differentiation. The quantity of each component provided in the kit is estimated to be sufficient for the induction of 3 x 107 ES cells. This kit has been shown to generate an average of 15 plus/minus 5% DA neurons as estimated from double labeling various mouse ES cell lines with antibodies specific to tyrosine hydroxylase and neuron-specific β-III tubulin.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
... ,Schistosomal glutathione-S-transferase (GST) is commonly used ... in E. coli (1). The GSTTag sequence ... and in some cases the solubility of ... soluble, properly folded form, GSTTag fusion proteins ...
... compatible with BioTeks microplate washers, dispensers ... use and walkaway automation are guaranteed ... used with the ELx405 washer or ... small footprint fits easily in most ...
... fast and versatile automated strip washer which ... 16-channel manifold. This breakthrough design allows for ... manifolds for overfill washing and overflow protection ... problem of fitting dispense and aspirate tubes ...
... The 820-MS features Varians unique ... provides simple, routine removal of troublesome ... more flexible interference-free analysis without the ... quadrupole ion guides, or cells. And, ...
Biology Products:
(Date:5/24/2016)... May 24, 2016 Ampronix facilitates superior patient care by providing unparalleled technology ... LCD display is the latest premium product recently added to the range of products ... ... ... 3d Imaging- LCD Medical Display- Ampronix News ...
(Date:5/3/2016)... May 3, 2016  Neurotechnology, a provider of ... MegaMatcher Automated Biometric Identification System (ABIS) , a ... projects. MegaMatcher ABIS can process multiple complex biometric ... combination of fingerprint, face or iris biometrics. It ... and MegaMatcher Accelerator , which have ...
(Date:4/19/2016)... , UAE, April 20, 2016 ... be implemented as a compact web-based "all-in-one" system solution ... the biometric fingerprint reader or the door interface with ... of modern access control systems. The minimal dimensions of ... ID readers into the building installations offer considerable freedom ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... A person commits a crime, and the detective ... the criminal down. An outbreak of foodborne illness ... (FDA) uses DNA evidence to track down the bacteria that ... It,s not. The FDA has increasingly used a complex, cutting-edge ... illnesses. Put as simply as possible, whole genome sequencing is ...
(Date:6/23/2016)... FRANCISCO , June 23, 2016   EpiBiome ... has secured $1 million in debt financing from Silicon ... ramp up automation and to advance its drug development ... its new facility. "SVB has been an ... beyond the services a traditional bank would provide," said ...
(Date:6/23/2016)... 23, 2016  Blueprint Bio, a company dedicated to ... medical community, has closed its Series A funding round, ... "We have received a commitment from Forentis ... need to meet our current goals," stated Matthew ... runway to complete validation on the current projects in ...
(Date:6/23/2016)... ... June 23, 2016 , ... Velocity Products, a division of ... and optimized exclusively for Okuma CNC machining centers at The International Manufacturing Technology ... among several companies with expertise in toolholding, cutting tools, machining dynamics and distribution, ...
Breaking Biology Technology: