Navigation Links
Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems

ProductsMouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
Company R&D Systems
Item Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation
Price $1,000.00
Description Reagents for the differentiation of mouse embryonic stem cells to dopaminergic neurons.

Dopamine cell bodies exist in the midbrain, where they are mainly located in the substantia nigra, the ventral tegmental area, and the retrorubral field. These dopaminergic (DA) neurons give rise to extensive forebrain dopamine innervation ( e.g.projections to the caudate nucleus, putamen nucleus accumbens, olfactory tubercle, and several cortical regions and limbic regions, including the amygdala, lateral septum and ventral hippocampus). The basic organization of midbrain dopamine neurons and their projections is consistent across most mammalians. The midbrain dopamine projections to the striatum have been among the most extensively studied catecholamine neurons, in part because the degeneration of DA neurons in the substantia nigra results in Parkinsons disease. Both animal models and clinical trials have suggested that cell replacement therapies may be effective in treating Parkinsons disease. This approach is limited, however, by the availability of a rich, effective source of neural precursors for DA neuron generation.

Embryonic stem (ES) cells, derived from the inner cell mass of pre-implantation embryos, have been recognized as the most pluripotent stem cell population. ES cells give rise to a collection of differentiated cells and tissues, including neurons. Thus, they may serve as a potential source of replacement DA neurons in Parkinsons disease. Two groups have reported methods for generating DA neurons from ES cells, thus demonstrating the feasibility of identifying conditions required for functional DA neuron differentiation of ES cells. Experimental models established from these studies might facilitate the in vitroexamination of various aspects of neural development, in particular the underlying mechanisms controlling cell lineage and fate commitment.

DESIGN OF THE KIT

The Mouse Dopaminergic Neuron Differentiation Kit is a system designed for in vitroneural differentiation of mouse ES cells in a serum-free environment. This kit contains specially formulated ITS and N-2 Plus Media Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to provide proper support for cell attachment and spreading. A growth factor panel, consisting of human fibroblast growth factor (FGF) basic, mouse FGF-8b, and mouse sonic hedgehog amino-terminal peptide (Shh-N), is included for effective DA differentiation. The quantity of each component provided in the kit is estimated to be sufficient for the induction of 3 x 107 ES cells. This kit has been shown to generate an average of 15 plus/minus 5% DA neurons as estimated from double labeling various mouse ES cell lines with antibodies specific to tyrosine hydroxylase and neuron-specific β-III tubulin.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
(3-[125I]iodotyrosyl26)Galanin, porcine, 370 kBq, 10 uCi. Category: IODINE LIGANDS....
Human FADD Affinity Purified Polyclonal Ab...
Human VEGF R3/Flt-4 Phycoerythrin MAb (Clone 54733)...
Immunogen: Synthetic Peptide: V(22) N G H E F E I E G E G E G R(36) Storage: -20 C, Avoid Freeze/Thaw Cycles...
Biology Products:
(Date:3/28/2017)... PUNE, India , March 28, 2017 ... (Analog, IP, Biometrics), Hardware (Camera, Monitors, Servers, Storage Devices), ... Maintenance), Vertical, and Region - Global Forecast to 2022", ... 30.37 Billion in 2016 and is projected to reach ... 15.4% between 2017 and 2022. The base year considered ...
(Date:3/23/2017)... 2017 The report "Gesture Recognition and Touchless Sensing Market ... - Global Forecast to 2022", published by MarketsandMarkets, the market is expected to ... between 2017 and 2022. Continue Reading ... ... ...
(Date:3/22/2017)... -- Vigilant Solutions , a vehicle location and ... today the appointment of retired FBI special agent ... development. Mr. Sheridan brings more than 21 ... on the aviation transportation sector, to his new role ... served as the Aviation Liaison Agent Coordinator (ALAC) in ...
Breaking Biology News(10 mins):
(Date:6/28/2017)... ... June 28, 2017 , ... ... UK Ltd (Bock) announced a strategic partnership where Bock will demonstrate DuPont’s ... OPTIMASH® AD-100, has been shown to help biogas producers in the agricultural sector ...
(Date:6/28/2017)... , ... June 28, 2017 , ... Supplies of the ... leading experts gathered at the Society of Nuclear Medicine and Molecular Imaging (SNMMI) 2017 ... technetium-99m (Tc-99m), which is used in 30 to 40 million nuclear medicine procedures worldwide ...
(Date:6/27/2017)... ... ... The recent vote by the American Medication Association to align with the World ... hopefully sheds new light on the way health insurers, governments and the public view ... of Texas Fertility Center . , “This designation is something we have ...
(Date:6/27/2017)... ... June 27, 2017 , ... Biodex Medical Systems, Inc. ... aid in one device. This assistive aid lifts patients safely from a seated to ... motorized lift mechanics. The Mobility Assist is the latest in a line of Biodex ...
Breaking Biology Technology: