Navigation Links
Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems

ProductsMouse Dopaminergic Neuron Differentiation Kit, 2nd Generation from R&D Systems
Company R&D Systems
Item Mouse Dopaminergic Neuron Differentiation Kit, 2nd Generation
Price $1,000.00
Description Reagents for the differentiation of mouse embryonic stem cells to dopaminergic neurons.

Dopamine cell bodies exist in the midbrain, where they are mainly located in the substantia nigra, the ventral tegmental area, and the retrorubral field. These dopaminergic (DA) neurons give rise to extensive forebrain dopamine innervation ( e.g.projections to the caudate nucleus, putamen nucleus accumbens, olfactory tubercle, and several cortical regions and limbic regions, including the amygdala, lateral septum and ventral hippocampus). The basic organization of midbrain dopamine neurons and their projections is consistent across most mammalians. The midbrain dopamine projections to the striatum have been among the most extensively studied catecholamine neurons, in part because the degeneration of DA neurons in the substantia nigra results in Parkinsons disease. Both animal models and clinical trials have suggested that cell replacement therapies may be effective in treating Parkinsons disease. This approach is limited, however, by the availability of a rich, effective source of neural precursors for DA neuron generation.

Embryonic stem (ES) cells, derived from the inner cell mass of pre-implantation embryos, have been recognized as the most pluripotent stem cell population. ES cells give rise to a collection of differentiated cells and tissues, including neurons. Thus, they may serve as a potential source of replacement DA neurons in Parkinsons disease. Two groups have reported methods for generating DA neurons from ES cells, thus demonstrating the feasibility of identifying conditions required for functional DA neuron differentiation of ES cells. Experimental models established from these studies might facilitate the in vitroexamination of various aspects of neural development, in particular the underlying mechanisms controlling cell lineage and fate commitment.

DESIGN OF THE KIT

The Mouse Dopaminergic Neuron Differentiation Kit is a system designed for in vitroneural differentiation of mouse ES cells in a serum-free environment. This kit contains specially formulated ITS and N-2 Plus Media Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to provide proper support for cell attachment and spreading. A growth factor panel, consisting of human fibroblast growth factor (FGF) basic, mouse FGF-8b, and mouse sonic hedgehog amino-terminal peptide (Shh-N), is included for effective DA differentiation. The quantity of each component provided in the kit is estimated to be sufficient for the induction of 3 x 107 ES cells. This kit has been shown to generate an average of 15 plus/minus 5% DA neurons as estimated from double labeling various mouse ES cell lines with antibodies specific to tyrosine hydroxylase and neuron-specific β-III tubulin.

Info R&D SystemsR&D Systems
R&D Systems, Inc.
614 McKinley Place N.E.
Minneapolis, MN 55413
Customer Service: 1 (800) 343-7475
1 (612) 379-2956

Fax Number: 1 (612) 656-4400
Web Site: http://www.rndsystems.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
(Date:7/22/2014)... A research team from the National Institute of ... Clinic, has demonstrated a dramatically improved technique for ... molecular vibration "signatures." The new NIST technique is ... Raman spectroscopy, but one that delivers signals that ... Raman scattering, and 100 times stronger than obtained ...
(Date:7/22/2014)... in modifying properties of nanomaterials for application in devices ... recent innovative studies conducted by scientists from the National ... a simple, efficient and low cost technique involving a ... Professor Sow Chorng Haur from the Department of Physics ... properties of two different types of materials can be ...
(Date:7/22/2014)... hear that drinking milk helps make their bones strong or that ... the idea that if something is good for us, it must ... Journal of Consumer Research , when children hear about the benefits ... predicted that when food is presented to children as making them ... learning how to read or count, they would conclude the food ...
Breaking Biology News(10 mins):Enhanced NIST instrument enables high-speed chemical imaging of tissues 2Enhanced NIST instrument enables high-speed chemical imaging of tissues 3NUS scientists use low cost technique to improve properties and functions of nanomaterials 2NUS scientists use low cost technique to improve properties and functions of nanomaterials 3
... crop that plays a key role in sustaining food security ... of the world, particularly drought-prone regions, it plays a central ... of people in Africa and Asia. To meet the ... desirable traits, such as higher yield and quality, disease resistance, ...
... to treat nausea and motion sickness, has the potential for new ... according to Dr. Vishal M. Gohil, Texas A&M AgriLife Research biochemist. ... drug has many potential new applications," Gohil said. "And now that ... to explore ways of using it to treat other diseases. We ...
... Boston University School of Medicine (BUSM) suggests that epigenetics may ... relapse of cancer. The findings are published online in Volume ... The term epigenetics refers to the external modifications ... do not change the DNA sequence, but instead, they affect ...
Cached Biology News:2 grants to UC Riverside boost scientists' efforts in developing improved cowpea varieties 22 grants to UC Riverside boost scientists' efforts in developing improved cowpea varieties 32 grants to UC Riverside boost scientists' efforts in developing improved cowpea varieties 42 grants to UC Riverside boost scientists' efforts in developing improved cowpea varieties 52 grants to UC Riverside boost scientists' efforts in developing improved cowpea varieties 6Old drug may teach new tricks in treating infectious diseases, cancer 2Old drug may teach new tricks in treating infectious diseases, cancer 3BUSM researchers study epigenetic mechanisms of tumor metastasis for improved cancer therapy 2
(Date:7/22/2014)... , July 22, 2014 Hussey Copper ... MD-Cu 29 antimicrobial copper announces Pullman Regional Hospital,s ... first EPA-registered antimicrobial solid touch surface, at a nominal ... of MRSA, E-coli and other bacteria within 2 hours of ... protecting against bacteria and infectious diseases in its facilities. ...
(Date:7/22/2014)... Md. , July 22, 2014  United ... today that it will release its second quarter ... July 29, 2014. United Therapeutics will ... 2014, at 9:00 a.m. Eastern Time.  The teleconference ... dialing 1-970-315-0533.  A rebroadcast of the teleconference will ...
(Date:7/22/2014)... 2014 Dr. Williams says, “Bush has ... recognized that the greatest health care system in the ... needlessly deadly while trying to serve the public. Unfortunately, ... to transform an enormous system that none of them ... his continuing series of Open Letters ( http://is.gd/wdU2CM ) ...
(Date:7/21/2014)... The yield so far is small, but chemists ... solution-based mineral substitution process to make a precursor to ... and alternative energy devices. , A paper describing the ... 21 issue of the journal Inorganic Chemistry , ... inorganic and nuclear chemistry fields. The paper was chosen ...
Breaking Biology Technology:Pullman Regional Hospital changes more than 1,100 touch points to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 2Pullman Regional Hospital changes more than 1,100 touch points to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 3Dr. Ira Williams Asks What’s Wrong With Jonathan Bush’s Book, Where Does It Hurt? 2Oregon chemists eye improved thin films with metal substitution 2