Navigation Links
Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC

ProductsMouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11 from MitoScience LLC
Company MitoScience LLC
Item Mouse Anti-Porcine Pyruvate Dehydrogenase (PDH), complex Immunocapture Kit Monoclonal Antibody, Protein G-Agarose Conjugated, Clone 15D3G9C11
Price $295.00
Description 250 g monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 g of PDH complex from heart mitochondria. Also included are 2 mg of bovine heart mitochondria for control immunocapture. The pyruvate dehydrogenase complex (PDH) immunocapture kit allows isolation of PDH from small amounts of tissue. This facilitates the subsequent analysis of the assembly state, activity and post-translational modification of the complex. Pyruvate dehydrogenase is a key enzyme in energy metabolism. It converts pyruvate that has been transported into the mitochondrion into the 2-carbon molecule acetyl-CoA for subsequent breakdown in the Krebs cycle (citric acid cycle). PDH activity is under tight control through phosphorylation and dephosphorylation of the E1 alpha subunit, reactions which involve a set of specific pyruvate dehydrogenase kinases (PDK1-4) and phosphatases respectively. The immunocapture antibody isolates a functionally active complex and a protocol has been described for measuring this activity while the enzyme is still bound to the capture beads or fixed in a 96 well plate. Tightly bound kinases and phosphatases are retained on the complex through the immunocapture step. Release of the protein and subsequent gel electrophoresis allows characterization of the phosphorylation state, which can then be analyzed in more detail by mass spectrometry. Uses for this PDH immunocapture kit include but are not limited to examining alterations in assembly of the complex in genetic PDH deficiency altered PDH functioning in late-onset diabetes (NIDMM) and the response of the enzyme complex to diet, exercise and sepsis.
Info MitoScience LLCMitoScience LLC
1850 Millrace Drive
Suite 3A
Eugene, Oregon 97403
Customer Service: (800) 910-6486
Fax Number: (541) 284-1801
Web Site: http://www.mitosciences.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Mouse Anti-Amodiaquine Monoclonal Antibody, Unconjugated, Clone 6D10 from AntibodyShop A/S
2. Mouse Anti-Protein S Monoclonal Antibody, Unconjugated, Clone HYB 232-02 from Abcam
3. Mouse Anti-Mouse Glutathione S-transferase M1 (GSTM1) Monoclonal Antibody, Unconjugated from Lifespan Biosciences
4. Mouse Anti-Human S-100 alpha/beta chain Monoclonal Antibody, Unconjugated, Clone 8B10 from Santa Cruz Biotechnology, Inc.
5. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Testis from OriGene Technologies
6. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Brain (adult) from OriGene Technologies
7. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies
8. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies
9. Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies
10. Mouse Anti-Human Complement factor B Monoclonal Antibody, Unconjugated, Clone 9B6 from AntibodyShop A/S
11. Mouse Anti-Human Choriongonadotropin (hCG) Monoclonal Antibody, Unconjugated, Clone 5F10 from AntibodyShop A/S
... tool provides the firm pressure necessary ... in tubes or plates. The multifunctional ... that firmly holds thin-wall 0.5 ml ... opening or closing. A side slot ...
... Gene Pulser Xcell microbial system, 100 ... used for electroporation of bacterial and ... main unit, PC module for exponential ... sterile cuvettes (5 each of 0.1 ...
... Clear yellow solution. In ... thimerosal, and other proprietary ... Diluent formulated to stabilize ... antibody conjugates at high ...
... Pulser Xcell eukaryotic system, 100 ... is used for electroporation of ... the main unit, CE module ... or square-wave pulses, ShockPod shocking ...
Biology Products:
(Date:3/20/2015)... Mar. 19, 2015 Research and Markets ( ... Geometry - Global Strategic Business Report" report to their ... Hand Geometry in US$ Thousands. The report provides separate comprehensive ... Japan , Europe , ... and Rest of World. Annual estimates and forecasts ...
(Date:3/17/2015)... , March 17, 2015 MedNet ... specializing in clinical study management systems, is pleased ... attendance at ARENA,s Outsourcing in Clinical Trials Southeast ... event provides an excellent forum for MedNet to ... and affordable software-as-a-service (SaaS) eClinical technology platform. ...
(Date:3/12/2015)... 12, 2015 The mascots of the Atlantic ... part of Wake Forest Baptist Medical Center. They stopped ... 2015 ACC Men,s Basketball Tournament. Young patients enjoyed bonding ... getting their autographs. Brenner Children,s Hospital is ... the only children,s hospital in northwest North ...
Breaking Biology News(10 mins):Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4Connect With MedNet Solutions At Outsourcing In Clinical Trials Southeast 2
... Northwestern Medicine scientists have identified a component ... cells, allowing the virus to rapidly and successfully ... Led by Gregory Smith, associate professor in immunology ... Medicine, researchers found that viral protein 1-2, or ...
... carry an abundance of bacteria on their surfaces, not all ... the variety of these non-pathogenic bacteria, scientists report that these ... cultivation practices. The results are published March 27 in the ... and Noah Fierer at the University of Colorado, Boulder. ...
... of Medicine at Mount Sinai is leading an international team ... to gain a better understanding of Alzheimer,s disease (AD). As ... Noggle, PhD, the NYSCF Charles Evans Senior Research Fellow ... Cell Foundation (NYSCF),s laboratory in Manhattan. Dr. Gandy heads ...
Cached Biology News:How herpesvirus invades nervous system 2Mount Sinai leads global program using stem cells to accelerate cures for Alzheimer's disease 2
(Date:3/31/2015)... 2015 Lighthouse Information Systems, Inc., ... in California, Oregon, Washington and clients across the ... and Acquisition (M&A) Integration Services offering, effective immediately. ... Services and M&A Integration Practice Leader at Lighthouse, ... majority of M&A initiatives fail to achieve their ...
(Date:3/30/2015)... , March 30, 2015  Multiple new research abstracts ... several important health factors. From their impact on colon ... positive effect on both gut health and vascular health, ... detail our latest understanding of walnuts, inner workings. Running ... , this annual meeting attracts an international audience ...
(Date:3/30/2015)... NEW BRUNSWICK, N.J. , March 30, 2015 /PRNewswire-USNewswire/ ... research and innovation projects in the state of ... to its next round of grant funding, announced ... existing New Jersey Health Foundation Research Grants program, an ... to $35,000 each to fund early stage research projects ...
(Date:3/30/2015)... OTTAWA , March 30, 2015 /CNW/ - A ... today on the salaries and demographic profiles of new ... "Opening the Door – Building Careers for New Grads ... Canada,s Research-Based Pharmaceutical Companies (Rx&D) and features ... federal Career Focus wage subsidy program from 2013 to ...
Breaking Biology Technology:Lighthouse Information Systems, Inc., Announces Addition of M&A Integration Services 2New Findings Support the Benefits of Eating Walnuts on Overall Health 2New Findings Support the Benefits of Eating Walnuts on Overall Health 3New Findings Support the Benefits of Eating Walnuts on Overall Health 4New Jersey Health Foundation Announces An Additional $1 Million To Fund Innovation and Research Grants in New Jersey 2New Report on Graduates in Canadian Biotech Industry Sheds Light on Hiring Practices 2New Report on Graduates in Canadian Biotech Industry Sheds Light on Hiring Practices 3New Report on Graduates in Canadian Biotech Industry Sheds Light on Hiring Practices 4