|Products||Mouse Anti-Nop1p / Fibrillarin Monoclonal Antibody, Unconjugated, Clone A00104.01 from GenScript Corporation|
|Item||Mouse Anti-Nop1p / Fibrillarin Monoclonal Antibody, Unconjugated, Clone A00104.01|
|Description|| Mouse Anti Nop1p/Fibrillarin (monoclonal) |
Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throghout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.
Antigen: Raised against yeast fibrillarin, a highly conserved nucleolar protein present in small nucleolar ribonucleoprotein (snoRNP) complexes.
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Applications: For western blots of yeast protein samples: 1:1,000 (cell lysates) to 1:5,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods: 1:500 to 1:2,500. To detect mammalian fibrillarin on western blots by ECL: 1:250. For immunofluoscence on yeast cells: 1:500 to 1:2,500. For IF of mammalian cells: 1:250.
|Info|| GenScript Corporation|
120 Centennial Ave.
Piscataway, NJ 08854
Customer Service: (Toll Free) : (877) 436-7274
Fax Number: (732) 210-0262
Web Site: http://www.genscript.com/