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Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsLysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Lysozyme Fluorometric (Green) Assay Kit, EnzChek®
Description The EnzChek Lysozyme Assay Kit (E-22013) provides researchers with a sensitive assay to measure levels of lysozyme activity in solution. This fluorescence-based assay can detect lysozyme activity down to 20 U/mL (Figure 1). The assay measures lysozyme activity on Micrococcus lysodeikticus cell walls, which are labeled to such a degree that the fluorescence is quenched. Lysozyme action can relieve this quenching, yielding a dramatic increase in fluorescence that is proportional to lysozyme activity. The fluorescence increase can be measured using any spectrofluorometer, mini-fluorometer or fluorescence microplate reader that can detect fluorescein (Figure 2).

Lysozyme (muramidase) is a ubiquitous enzyme present in human serum, urine, tears, seminal fluid and milk. Lysozyme hydrolyzes b-(1-4)-glucosidic linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell well of a variety of microorganisms. Serum and urine lysozyme levels may be elevated in acute myelomonocytic leukemia (FAB-M4), chronic myelomonocytic leukemia (CMML) and chronic myelocytic leukemia (CML).1 Increased serum lysozyme activity is also present in tuberculosis,2 sarcoidosis, 2 megaloblastic anemias,3 acute bacterial infections, ulcerative colitis 4 and Crohns disease.2,4 Elevated lysozyme levels in urine and serum occur during severe renal insufficiency,5 renal transplant rejection,6 urinary tract infections,7 glomerulonephritis 5 and nephrosis.5

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
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