Navigation Links
ImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.

ProductsImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ImmunoPure IgM Purification Kit
Price 
Description IgM binds poorly to Protein A and, until recently, no readily available affinity chromatography product existed for one- step IgM purification. Standard methods for IgM purification generally are multi-step, tedious processes, or they are not effective for removing all of the major impurities present in IgM samples. Pierce has developed an easy-to-use affinity matrix and buffer system to purify IgM. This matrix, an immobilized mannan binding protein (MBP) support, is most effective for purifying mouse IgM from ascites. Purified IgM can be obtained from a single pass over the affinity column. Human IgM will bind to the support and yield a product at least 88% pure by HPLC upon elution. The purification of IgMs from other species and mouse serum has not yet been optimized. Studies in Pierce labs show that MBP does not bind F(ab)2 and Fab. Mannan Binding Protein is immobilized on two supports: agarose and UltraLink Biosupport Medium. The ImmunoPure Immobilized MBP is also available in a kit format the ImmunoPure IgM Purification Kit. The kit contains sufficient buffers to perform 10 purifications using a 5 ml column of Immobilized MBP. Pierces ImmunoPure IgM Purification Kit is easy-to-use and yields 90% pure mouse IgM (from ascites) with a very simple protocol. Immobilized MBP can be regenerated at least 10 times with no apparent loss of binding capacity. The figure below demonstrates the high purity of Immobilized MBP purified IgM from mouse ascites.

  • Immobilized MBP Column 5 ml
  • IgM Binding Buffer 800 ml
  • IgM Elution Buffer 500 ml
  • MBP Column Preparation Buffer 50 ml
  • Column Extender
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. STag Thrombin Purification Kit from Novagen
2. MagneGST™ Protein Purification System from Promega
3. PsiPort ™ Mass Directed Auto Purification Software from Shimadzu Biotech
4. DNA Spin Gel Extraction/PCR Purification Kit from Yorkshire Bioscience Ltd
5. MinElute 96 UF PCR Purification Kit (4) from QIAGEN
6. Rotofor Purification System, 100-120 V from Bio-Rad
7. Rotofor Purification System With Power Supply, 220-240 V from Bio-Rad
8. Protein Purification Plate Centrifuge compatible from ABgene
9. Acridinium Purification Buffer (10X) from Cayman Chemical
10. GST Expression and Purification Kit from BD Biosciences Pharmingen
11. Histidine-tagged Protein Purification Resin from R&D Systems
A collection of 42 spots with normal tissues from different organs of C57 mice, rat and human....
... The V-106 voice-coil scanning and positioning ... small samples have to be positioned ... high resolution. It provides superior responsiveness ... drives. The mechanics (X or XY ...
Improves spot morphology and DNA binding to the slide resulting in increased hybridization signal. ID Clarifier: Optimized spotting solution for use with SigmaScreen APS Coated Slides for Microarrays...
... General description: Humidity wells in the base ... chamber humidity and probe hydration around the ... eliminates the risk of waterbath contamination. This ... temperature waterbaths or hybridisation ovens. Linkage: This ...
Biology Products:
(Date:10/14/2014)... been millions of years since T. rex ... Ohio University scientists is breathing life back into dinosaurs ... snouts. The research has important implications for how dinosaurs ... enhance the sense of smell and cool their brains. ... University doctoral student Jason Bourke, lead author of the ...
(Date:10/14/2014)... High doses of fish oil supplements, rich in omega-3 fatty ... irregular heartbeat in which the heart can beat as fast ... trial led by the Montreal Heart Institute were published in ... on October 7th. , For the trial, 337 patients with ... to 4 grams of fish oil a day or to ...
(Date:10/14/2014)... Pancreatic cancer likely takes between 10 and 20 ... very "broad window" of intervention if detected early, ... a predisposition, say Australian clinical researchers. , ... Sydney,s Garvan Institute of Medical Research, analysed medical ... of 766 pancreatic cancer patients, operated on between ...
Breaking Biology News(10 mins):Dinosaur breathing study shows that noses enhanced smelling and cooled brain 2Dinosaur breathing study shows that noses enhanced smelling and cooled brain 3Early detection window when pancreatic cancer is in the family 2
... Methodist University and The University of Texas at ... that shows promise in protecting brain cells against ... which afflict millions. Dallas-based startup EncephRx, Inc. ... owned compounds. A biotechnology and therapeutics company, EncephRx ...
... 2010 Polar research must become an integral part of ... from the dramatically changing face of the Polar Regions, the ... its strategic position paper on European polar research: "Relevance, Strategic ... activities in the Polar Regions are significant, amounting to over ...
... the emergency room after a fall. She has broken her ... screws or shiny titanium ball joints. Instead, she pulls out ... will solidify in seconds and injects into the break. Over ... natural growth factors embedded in the synthetic molecules of the ...
Cached Biology News:Novel compounds show early promise in treatment of Parkinson's, Huntington's, Alzheimer's 2Novel compounds show early promise in treatment of Parkinson's, Huntington's, Alzheimer's 3It's time for Europe to step up research in the polar regions 2In the lab, engineer's novel liquid provides a solid fix for broken bones 2
(Date:10/18/2014)... 2014 The Asian arthroscopic devices report ... analysis and forecast of revenue. The arthroscopic devices market ... by 2018, at a CAGR of 6.5% from 2013 ... Asian arthroscopic devices market, to get an idea of ... of the segmentation of arthroscopic devices market in the ...
(Date:10/18/2014)... October 18, 2014 The Asia-Pacific Liquid ... segments the concerned market in Asia-Pacific with analysis and ... million in 2013, and is expected to reach $380.0 ... 2013 to 2018. , Browse through the TOC of ... idea of the in-depth analysis provided. This also provides ...
(Date:10/18/2014)... October 18, 2014 The Biomass ... in the five years to 2014. The industry's expansion ... energy tax credits that encouraged the use of biomass ... portfolio standards (RPSs), which require local utilities to generate ... total energy portfolio. Increased campaigning for green technology also ...
(Date:10/18/2014)... The report " Cloud Analytics Market ... Speech; Machine; Video; Predictive Analytics: Global Advancements, Delivery ... Analysis (2013 - 2018) ", defines and segments ... segments with an in-depth analysis and forecasting of ... driving this market, various restraints, and opportunities impacting ...
Breaking Biology Technology:The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market is expected to reach $380.0 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Liquid Crystal on Silicon (LCoS) display market is expected to reach $380.0 million by 2018 - New Report by MicroMarket Monitor 3Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 2Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 3Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 2Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 3Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 4Cloud Analytics Market (by Cloud Business Intelligence & Predictive Analytics) Expected to Cross $16.52 Billion by 2018 - New Report by MarketsandMarkets 5