Navigation Links
ImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.

ProductsImmunoPure IgM Purification Kit from Pierce Biotechnology, Inc.
Company Pierce Biotechnology, Inc.
Item ImmunoPure IgM Purification Kit
Price 
Description IgM binds poorly to Protein A and, until recently, no readily available affinity chromatography product existed for one- step IgM purification. Standard methods for IgM purification generally are multi-step, tedious processes, or they are not effective for removing all of the major impurities present in IgM samples. Pierce has developed an easy-to-use affinity matrix and buffer system to purify IgM. This matrix, an immobilized mannan binding protein (MBP) support, is most effective for purifying mouse IgM from ascites. Purified IgM can be obtained from a single pass over the affinity column. Human IgM will bind to the support and yield a product at least 88% pure by HPLC upon elution. The purification of IgMs from other species and mouse serum has not yet been optimized. Studies in Pierce labs show that MBP does not bind F(ab)2 and Fab. Mannan Binding Protein is immobilized on two supports: agarose and UltraLink Biosupport Medium. The ImmunoPure Immobilized MBP is also available in a kit format the ImmunoPure IgM Purification Kit. The kit contains sufficient buffers to perform 10 purifications using a 5 ml column of Immobilized MBP. Pierces ImmunoPure IgM Purification Kit is easy-to-use and yields 90% pure mouse IgM (from ascites) with a very simple protocol. Immobilized MBP can be regenerated at least 10 times with no apparent loss of binding capacity. The figure below demonstrates the high purity of Immobilized MBP purified IgM from mouse ascites.

  • Immobilized MBP Column 5 ml
  • IgM Binding Buffer 800 ml
  • IgM Elution Buffer 500 ml
  • MBP Column Preparation Buffer 50 ml
  • Column Extender
Info Pierce Biotechnology, Inc.Pierce Biotechnology, Inc.
Pierce Biotechnology, Inc.
3747 N. Meridian Rd.
P.O. Box 117
Rockford, IL 61105
Customer Service: 815-968-0747
Fax Number: 815-968-8148
Web Site: http://www.piercenet.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. STag Thrombin Purification Kit from Novagen
2. MagneGST™ Protein Purification System from Promega
3. PsiPort ™ Mass Directed Auto Purification Software from Shimadzu Biotech
4. DNA Spin Gel Extraction/PCR Purification Kit from Yorkshire Bioscience Ltd
5. MinElute 96 UF PCR Purification Kit (4) from QIAGEN
6. Rotofor Purification System, 100-120 V from Bio-Rad
7. Rotofor Purification System With Power Supply, 220-240 V from Bio-Rad
8. Protein Purification Plate Centrifuge compatible from ABgene
9. Acridinium Purification Buffer (10X) from Cayman Chemical
10. GST Expression and Purification Kit from BD Biosciences Pharmingen
11. Histidine-tagged Protein Purification Resin from R&D Systems
(Date:5/14/2015)... , May 14, 2015 Research and Markets ... the "Fingerprint Sensors Market in Smart Mobile Devices ... report believes that 2014 was a watershed year for ... introduction of ,Apple Pay,. Apple gave fingerprint sensors a ... payment service. Fingerprint sensors are a must-have feature in ...
(Date:5/10/2015)... GOTHENBURG, Sweden , May 11, 2015 ... touch fingerprint sensors FPC1025 and FPC1155 from the distributor World ... one of FPC,s distributors in Asia . ... the sensors will be used by smartphone manufacturers in ... is included in the communicated revenue guidance of + 1 000 ...
(Date:4/27/2015)... 2015  For more than four decades, AUVSI,s ... the premier worldwide event for the unmanned land, sea, ... event for any local, national, or trade news organization ... markets and current applications of unmanned technologies. The conference ... $48 billion industry, and how it will soon impact ...
Breaking Biology News(10 mins):Global Smart Mobile Devices Fingerprint Sensors Market 2015-2019: Key Vendors are Authentec, Validity Sensors, Fingerprint Cards, Next Biometrics Group. IDEX, and Qualcomm 2Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 235 Million 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5
... Bottlenose dolphins that reside in designated Special Areas of ... might be at risk from pile driving. The frequency ... ability to communicate, find food and avoid predators. This ... their ability to breed successfully. Lactating females and young ...
... have long been encouraging Americans to add more fruits and ... discovered one way in which flavonoid-rich apples inhibit the kinds ... diseases, including heart disease and age-related cancers. , "We've known ... that are protecting the body. We just haven't known exactly ...
... computer cursors for fruit juice rewards have revealed that ... movements required for given actions, but rather the events ... is significant, said the researchers, because it yields new ... in this higher brain region, called the lateral prefrontal ...
Cached Biology News:Dolphins at risk 2UC Davis researchers reveal apples' protective ways 2Himalayan forests disappearing 2
(Date:5/26/2015)... 26, 2015 Regenestem Network ... cells clinic in Buenos Aires, Argentina. The clinic will ... care specialist. Pastrana has assembled a multidisciplinary team of ... in her state-of-the-art Puerto Madero office. , The ... to phase IV clinical studies in cellular therapies, and ...
(Date:5/26/2015)... HILDEN, Deutschland, May 26, 2015 ... ®   ermöglicht klinischen Laboren ... dazugehöriger Berichte; Vorstellung auf den Konferenzen ClinGen, ... N.V. (NASDAQ: QGEN ; Frankfurt Prime ... QIAGEN Clinical Insight ® (QCI™) bekannt. ...
(Date:5/26/2015)... GERMANTOWN, Md. , May 26, 2015   Intrexon ... biology, announced today that Gregory Frost , Ph.D., Senior ... at the Jefferies 2015 Global Healthcare Conference on Wednesday, June ... Grand Hyatt in New York City . ... XON ) is Powering the Bioindustrial Revolution with Better ...
(Date:5/26/2015)... Miami, FL (PRWEB) May 26, 2015 ... training, Inc. have announced plans for the company’s first ... 9, 2015, followed by a full day, hands-on stem ... hosted by J. Victor Garcia Gimenez, M.D., President of ... to the medical community’s increasing interest in regenerative medicine ...
Breaking Biology Technology:Regenestem Network and Silvina Pastrana, M.D. Announce the Opening of a New Stem Cell Clinic in Buenos Aires 2Regenestem Network and Silvina Pastrana, M.D. Announce the Opening of a New Stem Cell Clinic in Buenos Aires 3QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 2QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 3QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 4QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 5QIAGEN bringt klinische Bioinformatik-Plattform für Next Generation Sequencing-Anwendungen auf den Markt 6Intrexon to Present at the Jefferies 2015 Global Healthcare Conference 2Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 2Stem Cell Training, Inc. Announces First European Symposium on Cellular and Regenerative Medicine 3