|Products||Immobilized Metal affinity ProteinChip Arrays from Ciphergen|
|Item||Immobilized Metal affinity ProteinChip Arrays|
|Description|| Ciphergen's ProteinChip arrays have been developed for affinity capture of proteins from biological samples. A variety of ProteinChip chemical surfaces allow differential capture of proteins according to their unique biochemical properties. Each chip has eight different spots, allowing side-by-side sample comparison, and the chips are color-coded with a unique hydrophobic coating. Ciphergen's proprietary chip technology thus allows the researcher to rapidly capture, purify and characterize proteins directly on the chip.|
Immobilized metal affinity capture (IMAC) arrays bind proteins and peptides which have affinity for metals; proteins with exposed histidine, tryptophan and/or cysteine typically bind to metals immobilized on these chip surfaces. Binding occurs under pH 6-8 and high salt and decreases as the concentration of imidizole and glycine increase.
ProteinChip IMAC3: contains a nitriloacetic acid (NTA) surface for high-capacity nickel binding and subsequent affinity capture of proteins with metal binding residues. Imidazole may be used in binding and washing solutions to moderate protein binding, including binding of non-specific proteins. Increasing the concentration of imidazole in the washing buffers reduces the binding of the target proteins.
Ciphergen Biosystems, Inc
6611 Dumbarton Circle
Fremont, CA 94555
Customer Service: 510-505-2188
Fax Number: (510) 505-2101
Web Site: http://www.ciphergen.com