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Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM

ProductsHydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
Company BACHEM
Item Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2
Price $225.00
Description This quick (10 min) assay can detect H2O2 or peroxidase activity utilizing a non-fluorescent substrate, 10-acetyl-3,7-dihydroxyphenoxazine (ADHP). H2O2 oxidizes ADHP in a 1:1 stoichiometry to produce a fluorescent product resorufin (excitation at 530-571 nm, emission at 590-600 nm). This reaction is catalyzed by peroxidase in a one-step, homogeneous, no wash assay system. ADHP can be utilized to detect H2O2 release from cells or enzyme coupled reactions, and it allows monitoring of additional parameters. The assay is adaptable for HTS format. Application: Fluorescence via microwell plate reader.
Peninsula Laboratories Inc.
A Member of the Bachem Group
305 Old County Road
San Carlos, California 94070

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