Navigation Links
GenElute Agarose Spin Columns from Sigma-Aldrich

ProductsGenElute Agarose Spin Columns from Sigma-Aldrich
Company Sigma-Aldrich
Item GenElute Agarose Spin Columns
Price 
Description GenElute™ Agarose Spin Columns are designed for the rapid purification of linear 100 bp to 10 kb DNA from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel.

Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal recovery can be obtained with gel slices weighing less than 100 mg. A 40 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 40 l. A 90 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 65 l. The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease digestion, labeling and hybridization.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GenElute Minus EtBr Spin Column from Sigma-Aldrich
2. GenElute Gel Extraction Kit from Sigma-Aldrich
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. S-Protein Agarose from Novagen
6. UltraPure Agarose and SYBR Safe DNA Gel Stain Combo from Invitrogen
7. UltraPure Agarose and SYBR Safe DNA Gel Stain combo from Invitrogen
8. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
9. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
10. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
11. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Mycoplasma Removal Agent (MRA) has been developed for cell culture, to combat the widespread problems caused by contamination of cell cultures with mycoplasma....
... The product contains 5 ml ... with the monoclonal BerEP4 against ... (epithelial cell adhesion molecule). Also ... of 2 x 10e7 MNC ...
albumin from bovine serum (BSA), tetramethylrhodamine conjugate...
Superior homology search software - in all the flavours of blast, with increased sensitivity and speed...
Biology Products:
(Date:6/7/2016)... TORONTO , June 7, 2016  Syngrafii ... begun a business relationship that includes integrating Syngrafii,s ... pilot branch project. This collaboration will result in ... for the credit union, while maintaining existing document ... http://photos.prnewswire.com/prnh/20160606/375871LOGO ...
(Date:6/1/2016)... Favorable Government Initiatives Coupled With ... Identification to Boost Global Biometrics System Market Through 2021  ... report, " Global Biometrics Market By Type, By ... 2011 - 2021", the global biometrics market is projected ... of growing security concerns across various end use sectors ...
(Date:5/9/2016)... May 9, 2016 Elevay is ... to expanding freedom for high net worth professionals seeking ... today,s globally connected world, there is still no substitute ... ever duplicate sealing your deal with a firm handshake. ... by taking advantage of citizenship via investment programs like ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, former senior ... the University of North Carolina Kenan-Flagler Business School effective June 27. ... Kenan-Flagler, with a focus on the school’s international efforts, leading classes and participating ...
(Date:6/24/2016)... , June 24, 2016 Epic Sciences ... detects cancers susceptible to PARP inhibitors by targeting ... cells (CTCs). The new test has already been ... in multiple cancer types. Over 230 ... damage response pathways, including PARP, ATM, ATR, DNA-PK ...
(Date:6/23/2016)... WI (PRWEB) , ... June 23, 2016 , ... ... supplements, is pleased to announce the launch of their brand, UP4™ Probiotics, into ... for over 35 years, is proud to add Target to its list of ...
(Date:6/23/2016)... Houston Methodist Willowbrook Hospital has signed ... to serve as their official health care provider. ... will provide sponsorship support, athletic training services, and ... volunteers, athletes and families. "We are ... and to bring Houston Methodist quality services and ...
Breaking Biology Technology: