Navigation Links
GenElute Agarose Spin Columns from Sigma-Aldrich

ProductsGenElute Agarose Spin Columns from Sigma-Aldrich
Company Sigma-Aldrich
Item GenElute Agarose Spin Columns
Price 
Description GenElute™ Agarose Spin Columns are designed for the rapid purification of linear 100 bp to 10 kb DNA from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel.

Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal recovery can be obtained with gel slices weighing less than 100 mg. A 40 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 40 l. A 90 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 65 l. The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease digestion, labeling and hybridization.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GenElute Minus EtBr Spin Column from Sigma-Aldrich
2. GenElute Gel Extraction Kit from Sigma-Aldrich
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. S-Protein Agarose from Novagen
6. UltraPure Agarose and SYBR Safe DNA Gel Stain Combo from Invitrogen
7. UltraPure Agarose and SYBR Safe DNA Gel Stain combo from Invitrogen
8. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
9. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
10. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
11. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
... used as a fluorescent stain for nucleic ... orange at a concentration of 120 μM ... a sensitivity of 25-50 ng per band. ... cell staining of DNA in apoptosis studies. ...
Miscellaneous: Versatile stain for staining RNA (bluish-purple), DNA (blue), proteins (red) and acid polysaccharides Assay: ~ 95 % Suitab tst: differential staining of nucleic acids and prote...
...
... step procedure (PCR, Exonuclease III digestion and ... day. The typical cloning efficiency using this ... and Benefits: , , Universal - PCR ... vector , High Cloning Efficiency - Typically>80% ...
Biology Products:
(Date:4/26/2016)... Research and Markets has announced ... 2016-2020"  report to their offering.  , ,     (Logo: ... analysts forecast the global multimodal biometrics market to ... period 2016-2020.  Multimodal biometrics is being ... the healthcare, BFSI, transportation, automotive, and government for ...
(Date:4/15/2016)... -- Research and Markets has announced the ...  report to their offering.  ,      ... gait biometrics market is expected to grow at ... Gait analysis generates multiple variables such ... compute factors that are not or cannot be ...
(Date:3/31/2016)...   LegacyXChange, Inc. ... LegacyXChange is excited to release its first ... be launched online site for trading 100% guaranteed authentic ... also provide potential shareholders a sense of the value ... industry that is notorious for fraud. The video is ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... June 23, 2016   EpiBiome , a precision ... million in debt financing from Silicon Valley Bank (SVB). ... and to advance its drug development efforts, as well ... "SVB has been an incredible strategic partner ... a traditional bank would provide," said Dr. Aeron ...
(Date:6/23/2016)... , June 23, 2016 Apellis ... Phase 1 clinical trials of its complement C3 ... single and multiple ascending dose studies designed to ... (PD) of subcutaneous injection in healthy adult volunteers. ... (SC) either as a single dose (ranging from ...
(Date:6/23/2016)... ... 23, 2016 , ... ClinCapture, the only free validated electronic ... showcase its product’s latest features from June 26 to June 30, 2016 for ... Disrupting Clinical Trials in The Cloud during the conference. DIA (Drug Information ...
(Date:6/23/2016)... June 23, 2016 ReportsnReports.com ... report to its pharmaceuticals section with historic and ... and much more. Complete report on ... pages, profiling 15 companies and supported with 261 ... http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . The Global Cell ...
Breaking Biology Technology: