Navigation Links
GenElute Agarose Spin Columns from Sigma-Aldrich

ProductsGenElute Agarose Spin Columns from Sigma-Aldrich
Company Sigma-Aldrich
Item GenElute Agarose Spin Columns
Price 
Description GenElute™ Agarose Spin Columns are designed for the rapid purification of linear 100 bp to 10 kb DNA from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel.

Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal recovery can be obtained with gel slices weighing less than 100 mg. A 40 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 40 l. A 90 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 65 l. The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease digestion, labeling and hybridization.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. GenElute Minus EtBr Spin Column from Sigma-Aldrich
2. GenElute Gel Extraction Kit from Sigma-Aldrich
3. S-Protein Agarose from Novagen
4. S-Protein Agarose from Novagen
5. S-Protein Agarose from Novagen
6. UltraPure Agarose and SYBR Safe DNA Gel Stain Combo from Invitrogen
7. UltraPure Agarose and SYBR Safe DNA Gel Stain combo from Invitrogen
8. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
9. GELase LMP Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
10. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
11. GELase LMT Agarose Gel-Digesting Preparation from EPICENTRE Biotechnologies
Mouse monoclonal antibody raised against a partial recombinant WDR5. NCBI Entrez Gene ID = WDR5...
... Nonmuscle Myosin II Heavy Chain Isoform ... TBS + 0.03% Thimerosal) This antibody ... corresponding to the C-terminus of nonmuscle ... This antibody is effective in immunoblotting ...
... Anti-Lipid Phosphate Phosphatase 2 ... buffered aqueous solution ... saline containing 0.08% sodium ... from the human LPP2 ...
... Synaptogyrin 2 ( Abpromise ... Antigen: Synthetic ... sequence amino acids 42-57 of ... Entrez Gene ID: ...
Biology Products:
(Date:8/12/2020)... ... August 11, 2020 , ... Both Roche, based ... entered into license agreements with Housey Pharma’s HMI subsidiary to gain access to ... have annual Research and Development spending in excess of US $10 billion. , ...
(Date:8/7/2020)... LOUISVILLE, Ky. (PRWEB) , ... August 06, 2020 ... ... been selected to participate in the prestigious National STEM Scholar Program, a unique ... national network building, and project support for middle school science teachers nationwide. , ...
(Date:7/31/2020)... ... July 30, 2020 , ... BioFactura, Inc. today announced a ... Joint Science & Technology Office—Chemical and Biological Defense (JSTO-CBD) of the Department of ... a highly efficient mammalian cell culture-based bioprocess suitable to meet surge requirement needs ...
Breaking Biology News(10 mins):
(Date:7/31/2020)... ... ... eSource has long been touted as the solution to high data management ... reasons it did not take off as quickly as people initially expected, and where ... towards capturing data electronically for clinical trials and then repurposing it for downstream analysis, ...
(Date:7/18/2020)... ... July 16, 2020 , ... “We are thrilled to deliver this new ... only technology of its kind on the market and we were pleased that the ... capacity of traditional cultured ingredients, creating a natural way to extend the shelf life ...
(Date:7/10/2020)... ... July 09, 2020 , ... In most research ... to suppress pigment formation in zebrafish embryos, maintaining optical transparency to facilitate microscopic ... MA has been using the zebrafish model to investigate the causes of leukaemia ...
(Date:7/1/2020)... ... June 29, 2020 , ... The Interlocal ... contracts to its membership, recently named BioFit Engineered Products an Awarded ... to purchase ergonomic seating, cafeteria tables, book trucks and carts at discounted pricing ...
Breaking Biology Technology: