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GenElute Agarose Spin Columns from Sigma-Aldrich

ProductsGenElute Agarose Spin Columns from Sigma-Aldrich
Company Sigma-Aldrich
Item GenElute Agarose Spin Columns
Description GenElute™ Agarose Spin Columns are designed for the rapid purification of linear 100 bp to 10 kb DNA from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE, or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution, or enzymatic digestion of the agarose gel.

Typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. Recovery decreases with increasing fragment size. Optimal recovery can be obtained with gel slices weighing less than 100 mg. A 40 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 40 l. A 90 mg gel slice from a 1.0% gel will yield an eluate volume of approximately 65 l. The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease digestion, labeling and hybridization.
Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site:
NOTE: Price information is approximate list price and actual prices may vary.

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