|Products||EBY100 S. cerevisiae Strain from Invitrogen|
|Item||EBY100 S. cerevisiae Strain|
|Description||The pYD1 Yeast Display Vector is specifically designed to target and display recombinant proteins on the surface of Saccharomyces cerevisiae. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. pYD1 uses the a-agglutinin yeast adhesion receptor which consists of two domains Aga1 and Aga2 to display recombinant protein on the surface of S. cerevisiae. The gene of interest is cloned into the pYD1 vector in frame with the AGA2 gene. The resulting construct is transformed into the EBY100 S. cerevisiae strain (supplied transformed with Aga1). Aga1 and the Aga2-fusion protein associate within the secretory pathway and are displayed on the cell surface (Figure 1).|
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