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Dulbecco's Phosphate Buffered Saline (DPBS) from Sigma-Aldrich

ProductsDulbecco's Phosphate Buffered Saline (DPBS) from Sigma-Aldrich
Company Sigma-Aldrich
Item Dulbecco's Phosphate Buffered Saline (DPBS)
Price 
Description With 36 mg/L sodium pyruvate, 50 mg/L streptomycin sulfate, 100 mg/L kanamycin monosulfate, 1000 mg/L glucose, calcium chloride, and magnesium chloride.

The use of a balanced salt solution (BSS) in tissue culture is generally attributed to early workers in the field. In 1885, Sydney Ringer developed a solution of inorganic salts designed to maintain contractility of mammalian heart tissue. A less specific salt solution was designed by Tyrode for use in work with mammalian cells in general. Tyrodes salt solution became the accepted fluid for diluting protein components of media of natural origin. Since that time, many other balanced salt solutions have been developed for use in tissue culture. The current role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

  • serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance;
  • provides cells with water and certain bulk inorganic ions essential for normal cell metabolism;
  • combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism;
  • provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Sterile, Cell culture tested

Info Sigma-AldrichSigma-Aldrich
Sigma-Aldrich Corp.
St. Louis, MO, USA
Customer Service: 800-325-3010
Tech Support: 800-325-5832
Fax Number: 314-771-5757
Web Site: http://www.sigmaaldrich.com/
NOTE: Price information is approximate list price and actual prices may vary.

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Component of GELase™ Agarose Gel-Digesting Preparation. 1 ml of 50X Buffer is sufficient for replacing the buffer in at least 5 g of gel....
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Form: Concentrated Applications: ELISA...
Form: Ready to use Applications: ELISA...
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