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DNase I (RNase-free) (2 U/l) from Ambion

ProductsDNase I (RNase-free) (2 U/l) from Ambion
Company Ambion
Item DNase I (RNase-free) (2 U/l)
Price $340.00
Description DNase I (RNase-free) (E.C. 3.1.21.1) is a nonspecific endonuclease that degrades double-and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ambion's RNase-free DNase I is of the highest purity available and is recommended to degrade DNA in the presence of RNA when the absence of RNase is critical to maintain the integrity of the RNA. For example, DNase I is frequently used to remove template DNA following in vitro transcription, and to remove contaminating DNA in total RNA preparations (especially those from transfected cells which may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. DNase I requires bivalent cations (Mg2+ and Ca2+ at approximately 5 mM and 0.5 mM, respectively) for maximal activity and has a pH optimum of 7.8.
Info AmbionAmbion
2130 Woodward
Austin, TX 78744
USA

Call Ambion to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-512-795-4221
Customer Service: 512-651-0200
Fax Number: 512-651-0201
Web Site: http://www.ambion.com
NOTE: Price information is approximate list price and actual prices may vary.

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