Navigation Links
CpGenome™ Fast DNA Modification Kit from CHEMICON

ProductsCpGenome™ Fast DNA Modification Kit from CHEMICON
Company CHEMICON
Item CpGenome™ Fast DNA Modification Kit
Price $150.00
Description The CpGenome Fast DNA Modification Kit contains reagents required to perform a bisulfite modification on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminated and sulfonated, converting them to uracils, while 5-methylcytosines remain unaltered. Thus, the sequence of the treated DNA will differ depending on whether the DNA is originally methylated or unmethylated. Also, the initially complementary DNA strands will no longer be complementary after cytosine conversion. Primers for use in MSP can be designed to specifically amplify either a bisulfite-sensitive, unmethylated strand or a bisulfite-resistant, methylated strand, based upon these chemically-induced differences. • The PCR primers are designed to specifically amplify the promoter regions of the gene of interest. If the sample DNA was originally unmethylated, an MSP reaction product will be detectable when using the primer set (labeled as 'U') designed to be complementary to the unmethylated bisulfite converted DNA sequence. No product will be generated using a primer set (labeled as 'M') designed to be complementary to the derivative methylated DNA sequence. Conversely, an MSP product will be generated only using the M primer set if the sample was originally methylated, and the U primers will not amplify such a template.

  • Components: DNA Modification Reagent 6 g
    Binding Buffer 20 mL
    Wash Buffer* 12 mL
    Elution Buffer 1.5 mL
    DNA Modification Columns 25
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. CpGenome™ Universal DNA Modification Kit from Upstate
2. Nutrient Mixture F-12 Ham, Coons Modification (Hams F-12 Coons) from Sigma-Aldrich
3. Antibody Modification Services from Genescript
4. GIBCO Improved MEM Zn++ Option (Richters Modification) liquid from Invitrogen
5. EcoR I/Hind III End Modification Kit from Novagen
6. GIBCO BGJb Medium (Fitton-Jackson Modification) (1X) liquid from Invitrogen
7. GIBCO F-12K Nutrient Mixture Kaighns Modification (1X) liquid from Invitrogen
8. F-12K Nutrient Mixture Kaighns Modification (1x) liquid from Invitrogen
9. ES Cell Characterization Kit from CHEMICON
10. In Vitro Osteogenesis Assay Kit from CHEMICON
11. Human / Mouse / Rat Blood Vessels (CD31 / Von Willebrand Factor (vWF)) IHC staining Kit from CHEMICON
... experimental process of determining the nucleotide sequence ... Sequencing is based on Sangers enzymatic method ... cycle sequencing reactions. The resulting fluorescent products ... instrument controlled by a computer and the ...
... (pTyr 192 ) The antibody is preadsorbed to ... isolated antibody buffered aqueous glycerol solution ... (without Mg 2+ and Ca 2+ ), ... mg/mL BSA (IgG, protease free) and 0.05% sodium ...
... maintenance, calibration, and validation (MCV) ... plate used for hands-free startup, ... using the Bio-Plex suspension array ... for use with Bio-Plex Manager ...
High-throughput DNA sequencing of any library. Just give us your transformation mixture, and we'll do the rest. ABI 3730xl's yield the longest read lengths available. LIMS tracking utilizing Geospiza...
Biology Products:
(Date:6/9/2016)... innovation leader in attendance control systems is proud to announce the introduction of fingerprint ... sure the right employees are actually signing in, and to even control the opening ... ... ... Photo ...
(Date:6/3/2016)... 2016 Das DOTM ... Nepal hat ein 44 Millionen ... Kennzeichen, einschließlich Personalisierung, Registrierung und IT-Infrastruktur, an ... und Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte internationale ... teilgenommen, aber Decatur wurde als konformste und ...
(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... , June 27, 2016  Sequenom, Inc. (NASDAQ: ... enabling healthier lives through the development of innovative products ... the United States denied its ... the claims of Sequenom,s U.S. Patent No. 6,258,540 (",540 ... criteria established by the Supreme Court,s Mayo Collaborative Services ...
(Date:6/27/2016)... , June 27, 2016  Liquid Biotech ... the funding of a Sponsored Research Agreement with ... tumor cells (CTCs) from cancer patients.  The funding ... CTC levels correlate with clinical outcomes in cancer ... data will then be employed to support the ...
(Date:6/24/2016)... NC (PRWEB) , ... June 24, 2016 , ... Researchers ... the most commonly-identified miRNAs in people with peritoneal or pleural mesothelioma. Their findings are ... to read it now. , Diagnostic biomarkers are signposts in the blood, lung ...
(Date:6/23/2016)... , June 23, 2016 A person commits a ... crime scene to track the criminal down. An ... Food and Drug Administration (FDA) uses DNA evidence to track ... Sound far-fetched? It,s not. The FDA has increasingly ... support investigations of foodborne illnesses. Put as simply as possible, ...
Breaking Biology Technology: