Navigation Links
CpGenome™ Fast DNA Modification Kit from CHEMICON

ProductsCpGenome™ Fast DNA Modification Kit from CHEMICON
Company CHEMICON
Item CpGenome™ Fast DNA Modification Kit
Price $150.00
Description The CpGenome Fast DNA Modification Kit contains reagents required to perform a bisulfite modification on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminated and sulfonated, converting them to uracils, while 5-methylcytosines remain unaltered. Thus, the sequence of the treated DNA will differ depending on whether the DNA is originally methylated or unmethylated. Also, the initially complementary DNA strands will no longer be complementary after cytosine conversion. Primers for use in MSP can be designed to specifically amplify either a bisulfite-sensitive, unmethylated strand or a bisulfite-resistant, methylated strand, based upon these chemically-induced differences. • The PCR primers are designed to specifically amplify the promoter regions of the gene of interest. If the sample DNA was originally unmethylated, an MSP reaction product will be detectable when using the primer set (labeled as 'U') designed to be complementary to the unmethylated bisulfite converted DNA sequence. No product will be generated using a primer set (labeled as 'M') designed to be complementary to the derivative methylated DNA sequence. Conversely, an MSP product will be generated only using the M primer set if the sample was originally methylated, and the U primers will not amplify such a template.

  • Components: DNA Modification Reagent 6 g
    Binding Buffer 20 mL
    Wash Buffer* 12 mL
    Elution Buffer 1.5 mL
    DNA Modification Columns 25
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. CpGenome™ Universal DNA Modification Kit from Upstate
2. Nutrient Mixture F-12 Ham, Coons Modification (Hams F-12 Coons) from Sigma-Aldrich
3. Antibody Modification Services from Genescript
4. GIBCO Improved MEM Zn++ Option (Richters Modification) liquid from Invitrogen
5. EcoR I/Hind III End Modification Kit from Novagen
6. GIBCO BGJb Medium (Fitton-Jackson Modification) (1X) liquid from Invitrogen
7. GIBCO F-12K Nutrient Mixture Kaighns Modification (1X) liquid from Invitrogen
8. F-12K Nutrient Mixture Kaighns Modification (1x) liquid from Invitrogen
9. ES Cell Characterization Kit from CHEMICON
10. In Vitro Osteogenesis Assay Kit from CHEMICON
11. Human / Mouse / Rat Blood Vessels (CD31 / Von Willebrand Factor (vWF)) IHC staining Kit from CHEMICON
alpha/beta-Tubulin Antibody Ship: Hot Store: -20 C...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
...
Biology Products:
(Date:8/28/2014)... alert the world to Global Warming, has called for ... underground. He says that Carbon Capture, combined with limits ... avoid global warming getting out of control over the ... made the call during his presentation to the International ... meeting to discuss Carbon Capture and Storage. , ...
(Date:8/28/2014)... world,s most important crop-producing countries will be fully saturated ... current trends continue, according to a new study led ... pest types can already be found in around half ... spread advances at its current rate, scientists fear that ... overwhelmed by pests within the next 30 years. , ...
(Date:8/27/2014)... the mysteries of the human genome was unveiled today ... most detailed comparison yet of how the genomes of ... The research, appearing August 28 in in the journal ... the three species, genomes is "read out," and how ... The results add billions of entries to a publicly ...
Breaking Biology News(10 mins):Global warming pioneer calls for CO2 to be taken from atmosphere and stored underground 2New study charts the global invasion of crop pests 2Encyclopedia of how genomes function gets much bigger 2Encyclopedia of how genomes function gets much bigger 3Encyclopedia of how genomes function gets much bigger 4
... Energy,s Savannah River National Laboratory and the Chernobyl Center,s ... issue of the Health Physics Journal entitled, ... Zone 25 Years After the Accident," (Vol. 101, ... Office of Environmental Management,s (EM) International Program, SRNL and ...
... Energy Department,s laboratories are making headway on two projects ... charges faster, lasts longer, runs more safely, and might ... Lithium batteries are used in a variety of everyday ... battery could also significantly increase the charge capacity of ...
... named 13 U.S. Department of Energy (DOE) researchers as ... and Engineers (PECASE). This is the highest honor bestowed ... who are early in their independent research careers. The ... a variety of fields - from research to help ...
Cached Biology News:Journal focuses on Savannah River National Labratory, Chernobyl Laboratory collaboration 2Journal focuses on Savannah River National Labratory, Chernobyl Laboratory collaboration 3National labs leading charge on building better batteries 2US Department of Energy PECASE recipients 2US Department of Energy PECASE recipients 3US Department of Energy PECASE recipients 4
(Date:8/28/2014)... Aug. 28, 2014   SunTrust Robinson Humphrey ... veterans to its equity research team in biotechnology ... expansion in equity research demonstrates our commitment to ... to enhance their investment decision making," said ... "We continue to make significant investments in differentiated ...
(Date:8/28/2014)... (PRWEB) August 27, 2014 Scientists, researchers, ... for SPIE Laser Damage 2014 symposium. Marking ... for high-power lasers will run 14-17 September. The event ... optics and photonics . , The premier conference for ... laser damage to optical materials will engage researchers and ...
(Date:8/28/2014)... August 28, 2014 Supplementing their ... a promotion for Eppendorf Safe Lock Centrifuge ... purchase the highest quality tubes at an affordable ... for customers doing chemical, medical, pharmaceutical, and life ... are not regularly able to afford Eppendorf products. ...
(Date:8/27/2014)... Aug. 27, 2014 Rhythm, a biopharmaceutical company ... deficiencies that result in metabolic disorders, announced today that ... with the U.S. Securities and Exchange Commission (SEC) relating ... its common stock. The number of shares to be ... not yet been determined. Citigroup and Cowen ...
Breaking Biology Technology:SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 2SunTrust Robinson Humphrey Expands Equity Research in Healthcare and Energy 3Laser Damage to Draw Researchers, Engineers, Scientists to 46th Annual Conference 2Laser Damage to Draw Researchers, Engineers, Scientists to 46th Annual Conference 3Eppendorf Safe Lock Centrifuge Tube Promotion Now Available at Major Pipette Distributor Pipette.com 2Eppendorf Safe Lock Centrifuge Tube Promotion Now Available at Major Pipette Distributor Pipette.com 3Rhythm Files Registration Statement for Proposed Initial Public Offering 2