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CpGenome™ Fast DNA Modification Kit from CHEMICON

ProductsCpGenome™ Fast DNA Modification Kit from CHEMICON
Company CHEMICON
Item CpGenome™ Fast DNA Modification Kit
Price $150.00
Description The CpGenome Fast DNA Modification Kit contains reagents required to perform a bisulfite modification on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminated and sulfonated, converting them to uracils, while 5-methylcytosines remain unaltered. Thus, the sequence of the treated DNA will differ depending on whether the DNA is originally methylated or unmethylated. Also, the initially complementary DNA strands will no longer be complementary after cytosine conversion. Primers for use in MSP can be designed to specifically amplify either a bisulfite-sensitive, unmethylated strand or a bisulfite-resistant, methylated strand, based upon these chemically-induced differences. • The PCR primers are designed to specifically amplify the promoter regions of the gene of interest. If the sample DNA was originally unmethylated, an MSP reaction product will be detectable when using the primer set (labeled as 'U') designed to be complementary to the unmethylated bisulfite converted DNA sequence. No product will be generated using a primer set (labeled as 'M') designed to be complementary to the derivative methylated DNA sequence. Conversely, an MSP product will be generated only using the M primer set if the sample was originally methylated, and the U primers will not amplify such a template.

  • Components: DNA Modification Reagent 6 g
    Binding Buffer 20 mL
    Wash Buffer* 12 mL
    Elution Buffer 1.5 mL
    DNA Modification Columns 25
Info CHEMICONCHEMICON
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
Fax Number: 800-437-7502
Web Site: http://www.chemicon.com
NOTE: Price information is approximate list price and actual prices may vary.

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