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CellSensor CRE-bla HEK 293T Cell Line from Invitrogen

ProductsCellSensor CRE-bla HEK 293T Cell Line from Invitrogen
Company Invitrogen
Item CellSensor CRE-bla HEK 293T Cell Line
Price 
Description The CellSensor CRE-bla HEK 293T cell line contains the b-lactamase reporter gene under control of the cAMP response element (CRE). To obtain the cell line the CRE-bla construct was transduced into HEK 293T cells by lentivirus. Subsequent flow cytometry was used to isolate clones responsive to forskolin stimulation (Figure 1). The CellSensor CRE-bla HEK 293T cell line can be used to build specific G-protein coupled receptor (GPCR) assays or to detect changes in intracellular cAMP levels.
Info InvitrogenInvitrogen
Invitrogen Corporation
1600 Faraday Ave.
Carlsbad, CA 92008

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Recommended for use as a cell culture substratum at 0.1-0.2 mg/cm 2 or 5-10 μl/cm 2 . Optimal concentration depends on cell type as well as the application or research objectives. Type B...
... FROM LIGHT. Useful in cell activation experiments when calcium ... TLC. Soluble in DMSO or EtOH. For ... stock in DMSO is recommended. Microliter amounts of this ... rapid mixing (e.g. 10 μl into 5 ml of ...
... poorly to Protein A and, until recently, no ... step IgM purification. Standard methods for IgM purification ... not effective for removing all of the major ... Pierce has developed an easy-to-use affinity ...
... 3′-(α-Amino- p- methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine, 2HCl ... FROM MOISTURE. Protein synthesis inhibitor. Inhibits ... nascent polypeptide chains. Induces DNA fragmentation ... leukemia cells. Purity: ≥98% by ...
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