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CellSensor CRE-bla HEK 293T Cell Line from Invitrogen

ProductsCellSensor CRE-bla HEK 293T Cell Line from Invitrogen
Company Invitrogen
Item CellSensor CRE-bla HEK 293T Cell Line
Price 
Description The CellSensor CRE-bla HEK 293T cell line contains the b-lactamase reporter gene under control of the cAMP response element (CRE). To obtain the cell line the CRE-bla construct was transduced into HEK 293T cells by lentivirus. Subsequent flow cytometry was used to isolate clones responsive to forskolin stimulation (Figure 1). The CellSensor CRE-bla HEK 293T cell line can be used to build specific G-protein coupled receptor (GPCR) assays or to detect changes in intracellular cAMP levels.
Info InvitrogenInvitrogen
Invitrogen Corporation
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Carlsbad, CA 92008

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... is a 20 amino acid synthetic peptide whose sequence corresponds to ... is (amino to carboxy terminus): V115 - L - P ... P - Q - V - P - N - E ... (The C-terminal cysteine has been added to facilitate conjugation and is ...
... The Rapid-Screen cDNA Library Panels were ... just three sets of PCRs to identify ... analysis is performed in a 96-well "Master ... from 5,000 clones. Having identified the positive ...
... Library Panels were designed for full-length gene ... PCRs to identify the desired cDNA clone. ... a 96-well "Master Plate," where each well ... identified the positive well(s) by gel electrophoresis ...
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