Navigation Links
Canine Pancreatic Microsomal Membranes from Promega

ProductsCanine Pancreatic Microsomal Membranes from Promega
Company Promega
Item Canine Pancreatic Microsomal Membranes
Price Inquire
Description Microsomal vesicles are used to study co-translational and initial post-translational processing of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes. In addition, processing and glycosylation events may be studied by the transcription/translation of the appropriate DNA in the TNT® Lysate Systems when used with Canine Pancreatic Microsomal Membranes(a). To assure consistent performance with minimal translational inhibition and background, microsomes have been isolated free from contaminating membrane fractions and stripped of endogenous membrane-bound ribosomes and mRNA (1). Membrane preparations are assayed for both signal peptidase and core glycosylation activities using two different control mRNAs. The two control mRNAs supplied with this system are the precursor for β-lactamase (or ampicillin resistance gene product) from E. coli and the precursor for α-mating factor (or α-factor gene product) from S. cerevisiae. • The Signal Sequence Control mRNA (E. coli β-lactamase) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for the E. coli gene encoding the precursor to β-lactamase (the ampicillin resistance gene product). The RNA is synthesized without a cap analog. This control mRNA is used to assay for signal peptidase activity and is supplied with the Canine Pancreatic Microsomal Membranes System. • The Core Glycosylation Control mRNA (S. cerevisiae α-factor) is transcribed by SP6 RNA polymerase from a plasmid bearing the coding region for the S. cerevisiae α-mating factor. The RNA is synthesized without a cap analog. This control mRNA is used to assay for core glycosylation activity and is supplied with the Canine Pancreatic Microsomal Membranes System.

Features:
Microsomes are stripped of endogenous membrane-bound ribosomes and mRNA to ensure consistent performance with minimal translational inhibition and background. Performance tested in rabbit reticulocyte lysate.

Applications:
Examination of signal peptide cleavage, membrane insertion, translocation and core glycosylation. • Compatible with the TNT® Lysate Systems, Rabbit Reticulocyte Lysate, and Flexi® Lysate.
Info PromegaPromega
2800 Woods Hollow Rd.
Madison, WI 53711-5399 USA
Customer Service: 800-356-9526
Tech Support: 1-800-356-9526
Fax Number: 800-356-1970
Web Site: http://www.promega.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Canine Endostatin EIA Kit from Neogen Corporation
2. Canine serum from Equitech-Bio
3. Normal Canine Serum - 100mL from Biomeda Corporation
4. Canine CCL2 (JE; MCP-1) ELISA development Kit, DuoSet from R&D Systems
5. Canine IL-10 ELISpot Kit from R&D Systems
6. Canine IL-4 ELISpot Kit from R&D Systems
7. Canine IL-10 ELISpot Development Kit from R&D Systems
8. Canine IL-4 ELISpot Development Kit from R&D Systems
9. Canine IFN-gamma ELISpot Development Kit from R&D Systems
10. Canine IFN-gamma ELISA Kit from R&D Systems
11. Canine Serum from CHEMICON
OmniSlide Heated Wash Module. 2 independent chambers with precise temperature control from ambient of 5C to 70C. Each chamber holds 1 slide rack (20 slides). Includes 2 x Wash Sleeves, 1 x Slide R...
... bioassays for interferons are cytopathic effect inhibition assays, ... Sample types include; tissue culture supernatents; serum from ... assays, one unit is defined as the quantity ... cytopathic effect of viral infection by 50%. ...
... effect inhibition assays, also known as CPE and ... supernatents; serum from PK studies and delivery formulations. ... as the quantity of interferon required per milliliter ... by 50%. All standards used in ...
OmniSlide thermal Cycler for slide applications. 20 slide capacity. Humidity Chamber. Slide Rack can be transferred directly to Wash Module to avoid handling individual slides....
Biology Products:
(Date:8/25/2015)... CITY and BELLEVUE, Wash. ... leader in advanced robotic systems, announced today it will ... unmanned ground vehicle (UGV), at the National Tactical Officers ... The Guardian S is the first-ever ... culmination of years of research and in-field trials and ...
(Date:8/20/2015)... The wearable technology market has traditionally ... healthy. However, wearable technology has the potential to ... improve diagnostic capabilities and therapeutic outcomes. Today, Leaf ... Technologies Conference 2015 , where Barrett Larson ... healthcare.    "Over the past decade, ...
(Date:8/19/2015)... , Aug. 19, 2015  VOXX International Corporation ... has entered into a Definitive Agreement to purchase ... iris authentication market leader EyeLock through an acquiring ... have a controlling interest in the acquiring entity. ... completion of due diligence.  Expanding on its existing ...
Breaking Biology News(10 mins):Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 2Leaf Healthcare Releases Video from Wearable Technology Conference on New Applications for Wearables in the Hospital Environment 3VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 2VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 3VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 4VOXX International Corporation Signs Definitive Agreement To Acquire The Assets And A Controlling Interest Of Biometric Leader EyeLock 5
... CHAMPAIGN, Ill. University of Illinois researchers are using ... weightiest questions in biology the relationship between cell mass ... computer engineering and bioengineering professor Rashid Bashir, published its results ... the National Academy of Sciences . "It,s merging micro-scale ...
... , LA JOLLA, CA-Fisetin, a naturally occurring compound ... slows the onset of motor problems and delays ... to researchers at the Salk Institute for Biological ... of Human Molecular Genetics , sets the ...
... ovarian cancer but new research from the Centenary Institute has ... new way that can break the resistance mechanism. Published ... (FTY720) had a potent effect in human ovarian cancer cells, ... chemotherapeutic drug currently available for the treatment of ovarian cancer. ...
Cached Biology News:Microsensors offer first look at whether cell mass affects growth rate 2Natural compound shows promise against Huntington's disease 2Natural compound shows promise against Huntington's disease 3New treatment to overpower drug resistance in ovarian cancer 2
(Date:8/28/2015)... ... August 28, 2015 , ... Proove ... at the International Spine Intervention Society’ s 23rd Annual Scientific Meeting ... 1st, 2015. With a focus on evidence-based research and clinical application, the event ...
(Date:8/28/2015)... ... August 28, 2015 , ... ... surgeon, Wade McKenna, DO, announced today that the use of sterile, dehydrated amniotic ... complete healing of an otherwise non-healing surgical knee wound. , The case involved ...
(Date:8/27/2015)... ... 2015 , ... Capital Edge Consulting , a nationwide consulting firm that ... their ranking as one of the top 5000 companies listed on the 2015 ... nation. , “It is truly an honor to be recognized as one of America’s ...
(Date:8/27/2015)... , Aug. 27, 2015 The ... a patient,s eyes—and life—were saved by doctors at Vanderbilt ... working with NNFF, NovaBay Pharmaceuticals and Dr. John ... Center at Seton Medical Center in ... treatment used a breakthrough approach, pioneered by Dr. Crew, ...
Breaking Biology Technology:Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 2Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 3Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 4Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3