|Products||BioPrime Plus Array CGH Indirect Genomic Labeling Module from Invitrogen|
|Item||BioPrime Plus Array CGH Indirect Genomic Labeling Module|
|Description|| The BioPrime Plus Array CGH Genomic Labeling Systems are a high-performance labeling kits that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH). Available in both indirect and direct labeling formats, the BioPrime Plus Array CGH Genomic Labeling Systems provide a flexible solution to your genomic labeling needs.|
Using the BioPrime Plus Array CGH Genomic Labeling Systems, you can expect:
High yields of fluorescently labeled genomic DNA • signal-to-noise ratios • detection of gene copy number variations.
These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5 and 35 exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor AHA fluorescent nucleotides. In the BioPrime Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor NHS ester (indirect labeling systems) prior to hybridization.
The BioPrime Core Module is found in all standard BioPrime and BioPrime Plus Systems and Modules. It contains exo-Klenow fragment (40 U/ l), 2.5X random primer solution (octamers), control DNA (Salmon Sperm), stop buffer, and distilled water. Store at -20C. The BioPrime Plus Systems and Modules contain the Alexa Fluor modified dNTP mixes or amine modified dNTP mixes and the Alexa Fluor NHS Esters. Store at -20C. The standard BioPrime and BioPrime Plus Array CGH Genomic Labeling Systems also contain purification columns and purification buffers.
1600 Faraday Ave.
Carlsbad, CA 92008
Tech Support: 800-955-6288 ext. 2
Fax Number: 760-603-7229
Web Site: http://www.invitrogen.com