Navigation Links
Apo-Direct Apoptosis Detection Kit from eBioscience

ProductsApo-Direct Apoptosis Detection Kit from eBioscience
Company eBioscience
Item Apo-Direct Apoptosis Detection Kit
Price $395.00
Description The APO-DIRECT Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), Fluorescein-deoxyuridine triphosphate and propidium iodide/RNase A solution for counterstaining the total DNA.

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. These DNA fragments can be extracted from apoptotic cells and result in the appearance of DNA laddering when the DNA is analyzed by agarose gel electrophoresis. The DNA of non-apoptotic cells that remains largely intact does not display this laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with directly conjugated fluorescein- deoxyuridine triphosphate nucleotides (FITC-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template-independent addition of deoxyribonucleoside triphosphates to the 3'-hydroxyl ends of double- or single-stranded DNA with either blunt, recessed or overhanging ends. A substantial number of these sites are available in apoptotic cells providing the basis for the method utilized in the APO-DIRECT Kit. Non-apoptotic cells do not incorporate significant amounts of the FITC-dUTP due to the lack of exposed 3'-hydroxyl DNA ends.
Info eBioscienceeBioscience
6042 Cornerstone Court West
San Diego, CA 92121
Customer Service: (888) 999-1371
Fax Number: (858) 642-2046
Web Site: http://www.ebioscience.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
2. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
3. ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
4. Apo-BrdU Apoptosis Detection Kit from eBioscience
5. MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
6. MitoLightApoptosis Detection Kit from CHEMICON
7. Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
8. Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
9. Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
10. Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)
11. MitoCapture Apoptosis Detection Kit from BioVision
... M. Walker (1996) • This book ... for studying proteins and peptides. Each ... step-by-step instruction, timesaving troubleshooting tips, alternative ... of reagents and suppliers. Topics covered ...
... Silver Staining Kit, 1. Select from ... and peptides on denaturing, native, and ... reproducibility with precast gels, buffer strips, ... to that of radioisotopes with ready-to-use ...
Whole blood is collected from healthy pigs (mixed breed, either sex) at the time of slaughter, refrigerated within 6 hours, processed within 24 hours and frozen at -20ºC....
... is collected from fasted Rhesus monkeys of mixed ... Available Anticoagulants: N-02: Citrate ... N-08: Potassium Oxalate N-10: ... ACD N-07: CPD N-09: ...
Biology Products:
(Date:12/13/2018)... ... 2018 , ... Biocompare, the leading online information resource for ... to Know about Antibody Manufacturing” . This educational resource is the third documentary ... film is to educate scientists about antibody production, validation, and responsible sourcing. The ...
(Date:12/6/2018)... , ... December 06, 2018 , ... ... selling instruments, consumables and laboratory contract services, throughout his career. During his recent ... a US Sales team and established the commercial laboratory operations in Brunswick, Maine. ...
(Date:12/5/2018)... ... December 03, 2018 , ... uBiome, ... PhD, assistant professor in the Division of Gastroenterology and Hepatology at UC San ... Medical Advisory Board. Joining the board of leading scientists and doctors from around ...
Breaking Biology News(10 mins):
(Date:1/11/2019)... ... ... Drawing on over 22 years of software development expertise, the live ... some of the key lessons that have driven customer satisfaction with their eClinical suite. ... find out how to ensure that you continue to be able to take advantage ...
(Date:1/8/2019)... ... 07, 2019 , ... Today AngelMD announced that it completed ... investment AngelMD has made into Noninvasix in the past year. , To reduce ... to directly, accurately, and noninvasively measure brain oxygenation in preterm babies in the ...
(Date:1/4/2019)... FRANCISCO (PRWEB) , ... January 01, 2019 , ... ... world’s first Microbiome Conference at the World Economic Forum Annual Meeting January 22-25, ... The conference is invitation-only. , The World Economic Forum Annual Meeting brings together ...
(Date:12/19/2018)... ... 19, 2018 , ... R3 Stem Cell is now offering ... in Las Vegas. Training includes facial rejuvenation, hair restoration and erectile dysfunction procedures ... aesthetics, having the training from expert, Board Certified providers is critical to instilling ...
Breaking Biology Technology: