Navigation Links
Apo-BrdU Apoptosis Detection Kit from eBioscience

ProductsApo-BrdU Apoptosis Detection Kit from eBioscience
Company eBioscience
Item Apo-BrdU Apoptosis Detection Kit
Price $395.00
Description The APO-BRDU Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells, including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counterstaining the total DNA.

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. These DNA fragments can be extracted from apoptotic cells and result in the appearance of DNA laddering when the DNA is analyzed by agarose gel electrophoresis. The DNA of non-apoptotic cells that remains largely intact does not display this laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates to the 3'-hydroxyl ends of double- or single-stranded DNA with either blunt, recessed or overhanging ends. A substantial number of these sites are available in apoptotic cells providing the basis for the method utilized in the APO-BRDU Kit. Recent evidence has demonstrated that Br-dUTP is more readily incorporated into the genome of apoptotic cells than are the deoxynucleotide triphosphates complexed to larger ligands like fluorescein, biotin or digoxigenin. This greater incorporation gives rise to a brighter flow cytometry signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody. Non-apoptotic cells do not incorporate significant amounts of the Br-dUTP due to the lack of exposed 3'-hydroxyl DNA ends.
Info eBioscienceeBioscience
6042 Cornerstone Court West
San Diego, CA 92121
Customer Service: (888) 999-1371
Fax Number: (858) 642-2046
Web Site: http://www.ebioscience.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Apo-Direct Apoptosis Detection Kit from eBioscience
2. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
3. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
4. ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
5. MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
6. MitoLightApoptosis Detection Kit from CHEMICON
7. Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
8. Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
9. Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
10. Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)
11. MitoCapture Apoptosis Detection Kit from BioVision
...
...
D.R. Harper and C. Grose (1994) • Includes: virus structure & replication, fundamental virology, immunology and pathogenesis, vaccines and antiviral drugs, novel diagnostic techniques, cloning a...
IHC Polymer Detection Kit (Rabbit DAB)150 slides...
Biology Products:
(Date:7/8/2015)... , N.J. and NEW YORK , July ... Guidepoint today announced BD & Guidepoint ... companies with free access to Guidepoint,s expert network services. ... that are developing cutting-edge technologies to improve healthcare delivery ... Guidepoint research manager, each start-up entrepreneur will be able ...
(Date:7/8/2015)... 8, 2015 The consumer products industry ... must have products such as wearables, smart wallets & other ... biometrics based devices continues to rapidly grow.  Biometrics & Wearable ... NXTD ), Google Inc. (NASDAQ: GOOG ), Apple Inc. ... GoPro, Inc. (NASDAQ: GPRO ) and Baidu Inc. (NASDAQ: ...
(Date:7/7/2015)... Calif. , July 7, 2015  Based ... market, Frost & Sullivan recognizes Credence ID, LLC ... Entrepreneurial Company of the Year Award. Credence ... to aid in its mission of offering enrollment ... populations globally. As Credence ID was formed by ...
Breaking Biology News(10 mins):BD and Guidepoint Team to Connect Healthcare Start-up Companies With Business and Scientific Experts 2BD and Guidepoint Team to Connect Healthcare Start-up Companies With Business and Scientific Experts 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 2Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 4Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 5Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 6Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5
... in 1990 to cut worldwide hunger in half by 2015. That ... and no action -- according to a Cornell University expert on ... people will be hungry in the world -- 800 million -- ... Cornell's Babcock Professor of Food, Nutrition and Public Policy and the ...
... $10.3 million to help develop a new prosthetic arm that would ... is a key part of a U.S. Department of Defense contract ... soldiers and potentially others whose arms were amputated. , "Imagine an ... allows you to feel both the outside world and your own ...
... are interested in understanding genetic control of grass inflorescence ... maize) provide most of the world's food. , Grass ... dictate most of the variation in form seen in ... tassel (male pollen-bearing flowers) and the ear (female flowers ...
Cached Biology News:World's pledge to halve hunger by 2015 looks like empty promise 2Both alcoholism and chronic smoking can damage the brain's prefrontal cortex 2Both alcoholism and chronic smoking can damage the brain's prefrontal cortex 3Both alcoholism and chronic smoking can damage the brain's prefrontal cortex 4Both alcoholism and chronic smoking can damage the brain's prefrontal cortex 5The evolution of food plants: Genetic control of grass flower architecture 2
(Date:7/27/2015)... 2015   Inocucor Technologies Inc ., the ... for farmers, has received a notice of allowance ... its patent application to protect Inocucor consortia and ... enhance yields on farms and in greenhouses. ... IN-M1 microbial consortium and its fermentation byproducts, which ...
(Date:7/27/2015)... , July 27, 2015 According ... by Type (Home-brew Media, Pre-formulated Media), by Application (Biobanking, Regenerative ... , Europe , Asia-Pacific ... 2019", The global biopreservation media market was valued at $247.7 ... $571.5 million by 2019, at a CAGR of 18.2% between ...
(Date:7/27/2015)... 27, 2015 IDEXX Laboratories, Inc. (NASDAQ: ... have submitted over 100,000 specimens to IDEXX Reference Laboratories ... first 10 days since the breakthrough kidney function test ... a leading cause of suffering and death in cats ... consistent with generally accepted kidney disease prevalence data showing ...
(Date:7/25/2015)... ... 2015 , ... Technology leader Anton Paar offers the ... surface temperature of the measuring prism. This is required to achieve the most ... are key requirements. It is likely that no refractometer manufacturers on the market ...
Breaking Biology Technology:Inocucor Technologies Receives Groundbreaking U.S. Patent for Proprietary Biological Processes that Stimulate Plant Growth 2Inocucor Technologies Receives Groundbreaking U.S. Patent for Proprietary Biological Processes that Stimulate Plant Growth 3Global Biopreservation Media Market to be Worth $571.5 Million by 2019 2Global Biopreservation Media Market to be Worth $571.5 Million by 2019 3Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 2Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 3Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 4Over 100,000 Pets Tested with New IDEXX SDMA Kidney Function Test Since Product Launch on July 13 5It’s T-Time for Refractometers: On-site Temperature Check Now Available 2