Navigation Links
Apo-BrdU Apoptosis Detection Kit from eBioscience

ProductsApo-BrdU Apoptosis Detection Kit from eBioscience
Company eBioscience
Item Apo-BrdU Apoptosis Detection Kit
Price $395.00
Description The APO-BRDU Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells, including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counterstaining the total DNA.

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. These DNA fragments can be extracted from apoptotic cells and result in the appearance of DNA laddering when the DNA is analyzed by agarose gel electrophoresis. The DNA of non-apoptotic cells that remains largely intact does not display this laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates to the 3'-hydroxyl ends of double- or single-stranded DNA with either blunt, recessed or overhanging ends. A substantial number of these sites are available in apoptotic cells providing the basis for the method utilized in the APO-BRDU Kit. Recent evidence has demonstrated that Br-dUTP is more readily incorporated into the genome of apoptotic cells than are the deoxynucleotide triphosphates complexed to larger ligands like fluorescein, biotin or digoxigenin. This greater incorporation gives rise to a brighter flow cytometry signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody. Non-apoptotic cells do not incorporate significant amounts of the Br-dUTP due to the lack of exposed 3'-hydroxyl DNA ends.
Info eBioscienceeBioscience
6042 Cornerstone Court West
San Diego, CA 92121
Customer Service: (888) 999-1371
Fax Number: (858) 642-2046
Web Site: http://www.ebioscience.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Apo-Direct Apoptosis Detection Kit from eBioscience
2. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
3. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
4. ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
5. MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
6. MitoLightApoptosis Detection Kit from CHEMICON
7. Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
8. Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
9. Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
10. Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)
11. MitoCapture Apoptosis Detection Kit from BioVision
Request Info...
... endotoxin-free plasmid DNA preparations are prepared ... quantities from 1mg to multi-gram scales. ... large-scale (100mg-multi-gram) preparations are made according ... is formulated according to customer needs, ...
... One can operate as a Voltage Clamp, ... (Dynamic) Clamp in electrophysiology research applications. Performance ... conventional analog equipment. This amazing instrument is ... capabilities of Digital Signal Processing (DSP) integrated circuit ...
... Cloning System facilitates the display of custom ... M13 as coat protein fusions, creating a ... its encoding DNA sequence. Peptide ligands for ... selected by the straightforward method of biopanning. ...
Biology Products:
(Date:11/12/2014)... Fla. , Nov. 12, 2014 Crossmatch™, ... today that its U.are.U ® fingerprint readers have ... chain in Central Mexico . The ... 2014 to eliminate payroll issues caused by employees clocking ... installing the U.are.U fingerprint readers, Montparnasse relied on paper ...
(Date:11/10/2014)... shelves in early 2010, and people have used them ... be tossed into a washing machine without ever having ... though, has come with risks for young children. , ... found that from 2012 through 2013, U.S. poison control ... years of age swallowing, inhaling, or otherwise being exposed ...
(Date:11/7/2014)... conjunction with the Glendale and ... the Verdugos has announced the " GLENDALE - ... to keep the Verdugo Regional Crime Lab operational after its ... existence, the Lab has processed more than 400 DNA samples, ... "Because our number one priority is the wellbeing of ...
Breaking Biology News(10 mins):Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2Study finds laundry detergent pods, serious poisoning risk for children 2Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2
... LA JOLLA, CA November 22, 2012 Scientists ... major advance in understanding how flu viruses replicate within ... electron-microscopy techniques to "see" one of influenza,s essential protein ... study show flu virus proteins in the act of ...
... have streamed braille patterns directly into a blind patient,s retina, ... an ocular neuroprosthetic device. The device, the Argus II, has ... now see color, movement and objects. It uses a small ... to translate the signal from the camera into electrical stimulation, ...
... for high-energy predators. And to survive, such marine predators need to ... will do, suggests a new study by researchers from the University ... Published today in the online journal PLOS ONE , the ... and dolphins depends on the quality of their diets and this ...
Cached Biology News:Scripps Research Institute scientists describe elusive replication machinery of flu viruses 2Scripps Research Institute scientists describe elusive replication machinery of flu viruses 3Blind patient reads words stimulated directly onto the retina 2
(Date:11/18/2014)... (PRWEB) November 17, 2014 RPS ... diagnostic tests – today announces its third annual ... Prevention’s (CDC) Get Smart About Antibiotics Week from ... is a national campaign designed to highlight the ... departments, and non-profit and for-profit partners to provide ...
(Date:11/18/2014)... 2014 pH measurement and control is ... pharmaceutical, chemical, and food and beverage industries. But what ... how do pH sensors work? METTLER TOLEDO has produced ... process industries with these questions and more. , METTLER ... the process industries. The actual sensor, the pH electrode, ...
(Date:11/18/2014)... Alanda Software, a leading global ... announced today that a top tier global biopharmaceutical ... solution in both the U.S. and EU markets. ... Data Quality remediation portal, designed to help both ... improve their ability to capture, transmit and receive ...
(Date:11/18/2014)... (PRWEB) November 18, 2014 Brothers Josh ... the launch of PAWSitively Curing Cancer, Inc . ... new 501(c) (3) non-profit organization is dedicated to raising ... goes directly to the University of Florida College ... with PAWSitively Curing Cancer as the recipient of this ...
Breaking Biology Technology:RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 3pH Guide from METTLER TOLEDO Explains Measurement, Theory and Practice of pH Applications 2Alanda Software Completes Successful Implementation of Global Data Quality & Vendor Management Solution for Top Tier Global Biopharmaceutical Company 2PAWSitively Curing Cancer, Inc. Launched By Two Children for National Pet Cancer Awareness Month 2PAWSitively Curing Cancer, Inc. Launched By Two Children for National Pet Cancer Awareness Month 3