Navigation Links
Apo-BrdU Apoptosis Detection Kit from eBioscience

ProductsApo-BrdU Apoptosis Detection Kit from eBioscience
Company eBioscience
Item Apo-BrdU Apoptosis Detection Kit
Price $395.00
Description The APO-BRDU Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells, including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counterstaining the total DNA.

One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. These DNA fragments can be extracted from apoptotic cells and result in the appearance of DNA laddering when the DNA is analyzed by agarose gel electrophoresis. The DNA of non-apoptotic cells that remains largely intact does not display this laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates to the 3'-hydroxyl ends of double- or single-stranded DNA with either blunt, recessed or overhanging ends. A substantial number of these sites are available in apoptotic cells providing the basis for the method utilized in the APO-BRDU Kit. Recent evidence has demonstrated that Br-dUTP is more readily incorporated into the genome of apoptotic cells than are the deoxynucleotide triphosphates complexed to larger ligands like fluorescein, biotin or digoxigenin. This greater incorporation gives rise to a brighter flow cytometry signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody. Non-apoptotic cells do not incorporate significant amounts of the Br-dUTP due to the lack of exposed 3'-hydroxyl DNA ends.
Info eBioscienceeBioscience
6042 Cornerstone Court West
San Diego, CA 92121
Customer Service: (888) 999-1371
Fax Number: (858) 642-2046
Web Site: http://www.ebioscience.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Apo-Direct Apoptosis Detection Kit from eBioscience
2. ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
3. ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
4. ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
5. MitoLight Mitochondrial Apoptosis Detection Kit from CHEMICON
6. MitoLightApoptosis Detection Kit from CHEMICON
7. Apoptosis Pathway SpliceArrays from ExonHit Therapeutics Inc.
8. Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
9. Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
10. Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)
11. MitoCapture Apoptosis Detection Kit from BioVision
... can be induced prior to DNA purification ... stability of single-copy BACs with the high ... vectors. The kits provide the linearized vector ... screening system) necessary to create a 10X ...
...
... 3T3 cells were cultured in DMEM with 4 ... of growth. In order to keep the ... in acetone-methanol. The cells are arrayed on ... each wells surface specifically treated to enhance cellular ...
... Systems provide rapid, precise localization of ... frozen or paraffin-embedded tissue, cytospins and ... facilitate double or triple labeleing experiments, ... with primary antibodies of different animal ...
Biology Products:
(Date:8/26/2014)... of mathematical sciences at New Jersey Institute of Technology ... biological clock that sets the pace for daily life. ... all other mammals with fundamental rhythms of life that ... day and with seasonal change. The brain,s circadian clock ... behavior which, when disrupted by experiences such as jet ...
(Date:8/26/2014)... is pumping far more pollution into the atmosphere than ... the National Center for Atmospheric Research estimates that more ... in such fires, emitting gases and particles that can ... new study provides the first rough estimates, on a ... and mercury that are emitted by the fires. Such ...
(Date:8/26/2014)... suggests the planet can produce much more land-plant biomass ... grains and other terrestrial plant parts than previously ... Environmental Science and Technology , recalculates the theoretical limit ... much higher than many current estimates allow. , "When ... you have to make some simplifying assumptions," said University ...
Breaking Biology News(10 mins):Researching fundamental rhythms of life 2Researching fundamental rhythms of life 3Trash burning worldwide significantly worsens air pollution 2Trash burning worldwide significantly worsens air pollution 3Study: Earth can sustain more terrestrial plant growth than previously thought 2Study: Earth can sustain more terrestrial plant growth than previously thought 3
... , Journal of Visualized Experiments , details the ... polymer, SurgiLux, was developed by scientists at the University ... from chitin, which is found in fungal cell walls ... allows SurgiLux to form low energy bonds between the ...
... Institute for Medical Research has been awarded a $1 ... which is administered by the National Institute for Occupational ... for Disease Control and Prevention (CDC), the National Institute ... comprehensively study the overall physical, mental and socioeconomic impact ...
... Manoel Manghi from Toulouse University in France and his colleagues ... to a double stranded molecule of DNA when it is ... be published in EPJ E . Instead of using ... settings, the authors focused on using a theoretical model to ...
Cached Biology News:New bio-adhesive polymer demonstrated in JoVE 2Feinstein Institute receives $1 million grant to study impact of World Trade Center attacks on responders 2
(Date:8/26/2014)... 2014 - A team of engineers from Singapore ... sensor network (WSN) for satellite communications. , With ... in satellite design, constructing a lightweight, low power-consuming, ... inside the satellite has always been a challenge ... the Satellite Research Centre of Nanyang Technological University ...
(Date:8/26/2014)... Aug. 26, 2014  ViveBio, LLC a leading ... stabilization and transportation, announced today that it has ... LLC (LPS) of Knoxville, Tennessee ... the United States . ViveST ... ambient temperature dried biological specimen transport systems.  The ...
(Date:8/26/2014)... Scranton, PA (PRWEB) August 26, 2014 ... San Jose, CA, which specializes in chemical process equipment, ... with Michigan State University’s Facility for Rare Isotope Beams ... ft. long precision cleaning line made with Vycom’s Flametec ... resonators, which are the essential parts of FRIB’s linear ...
(Date:8/26/2014)... , Aug. 26, 2014  Guardant Health, ... commercialization of oncology diagnostic products, has been recognized ... 2015 Technology Pioneer . As an innovator ... of 24 companies across a wide variety of ... Guardant360 ® , Guardant Health,s ...
Breaking Biology Technology:ZigBee in the Sky 2ViveBio and Laboratory Procurement Services Sign Agreement for Distribution of the ViveST Line of Products 2Modutek Selects Vycom’s Flametec Materials for Michigan State University’s Superconductor Equipment 2Modutek Selects Vycom’s Flametec Materials for Michigan State University’s Superconductor Equipment 3Guardant Health Named a 2015 Technology Pioneer by World Economic Forum 2