Navigation Links
Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Price 
Description a-Amylase is an enzyme that catalyzes the hydrolysis of starch to a mixture of maltose, maltotriose, and dextrins. The level of a-amylase in various fluids of the human body is of clinical significance in the diagnosis of disease states including pancreatitis and diabetes; plant and microbial a-amylases are important enzymes for industrial applications ranging from the manufacture of baked goods and dairy products to the production of ethanol and paper.14

The EnzChek Ultra Amylase Assay Kit (E33651) provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative the DQ starch substrate that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity (Figures 2 and 3) and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

In tests using a-amylase from Bacillus sp. (Sigma A-6380) and 200 g/mL of the DQ starch substrate (30 minute incubation at room temperature), the EnzChek Ultra Amylase Assay Kit could be used to detect a-amylase activity down to a final concentration of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
USA Canada 1-541-359-2658
Customer Service: 1-541-465-8300
Fax Number: 1-541-344-6504
Web Site: http://probes.invitrogen.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
2. Hydrogen Peroxide / Peroxidase Fluorometric (resorufin) Assay Kit, Fluoro H2O2 from BACHEM
3. 20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
4. Cell invasion Fluorometric (green) Assay Kit, InnoCyte™ from Calbiochem
5. Telomerase Fluorometric Assay Kit, TRAPeze from CHEMICON
6. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
7. TACE Fluorometric (MCA)) Assay Kit, InnoZyme™ from Calbiochem
8. Lysozyme Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
9. Cholesterol Fluorometric (Red) Assay Kit, Amplex Red from Molecular Probes (Invitrogen)
10. Pyrophosphate Fluorometric (Blue) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
11. MMP-1 Fluorometric (Blue) Assay Kit, EnzoLyte from AnaSpec
... Rabbit polyclonal to Rad51 ( ... Antigen: Synthetic ... to N terminal amino acids 15-29 ... Entrez Gene ID: 5888 ...
... Sequenase Dye Primer Manual Cycle Sequencing Kit ... dye-labeled primers and high-resolution fluorescence scanners. Sequencing ... standard slab gel electrophoresis and scanning of ... products. The kit components have been ...
... performance agitation and temperature control for microbiology ... wells independently and is suitable for DASGIP ... working volume. Its temperature ranges from approximately ... well is equipped with an individual electrical ...
... SYBR Green II RNA gel stain is ... bright fluorescence when bound to RNA and low ... with either formaldehyde/agarose or polyacrylamide gels using laser ... 50 minigels. A 1 mL unit size (S-7568) ...
Biology Products:
(Date:6/17/2015)... June 17, 2015  Synaptics Inc. (NASDAQ: ... today announced that Xiaomi, one of the world,s ... ClearPad ® family of capacitive touchscreen ... circuits (DDICs) for its latest smartphones, the Xiaomi ... for full in-cell display solutions and DDICs in ...
(Date:6/16/2015)... Fingerprint Cards has received an order ... company,s portfolio from one of its module partners in ... place during the third quarter 2015. The sensors will be ... Jörgen Lantto, CEO of FPC, comments: " This ... interest from smartphone OEMs in integrating touch fingerprint sensors in ...
(Date:6/15/2015)... Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company") a ... announces that its leading payment security technology, the Wocket® smart ... NYC June 18th. Digital Experience! @ NYC is ... to over 300 of the top media covering the sector. ... New York City from 6:00 to ...
Breaking Biology News(10 mins):Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3FPC Receives Order for Touch Fingerprint Sensors of 375 MSEK 2Wocket Smart Wallet Exhibiting at Pepcom Digital Experience! @ NYC June 18th 2Wocket Smart Wallet Exhibiting at Pepcom Digital Experience! @ NYC June 18th 3
... the Amazon before the arrival of Europeans, with little ... of research led by Crystal McMichael and Mark Bush ... overturns the idea the Amazon was a cultural parkland ... vast tracts of the landscape. The Amazon Basin ...
... PHILADELPHIA Healthy humans harbor an enormous and diverse ... their intestines. These microbial partners provide beneficial aid in ... development of a healthy immune system. In a new ... David Artis, PhD, associate professor of Microbiology, and Michael ...
... descend on Virginia Tech beginning June 24 bringing with them ... boost to the Blacksburg area in excess of $2 million. ... the largest conferences ever held in Blacksburg with 13 simultaneous ... molecules macromolecules that are having a huge impact ...
Cached Biology News:Scientists reconstruct pre-Columbian human effects on the Amazon Basin 2Brothers in arms: Commensal bacteria help fight viruses 2Scientific meeting at Virginia Tech will make more than $2 million economic impact 2Scientific meeting at Virginia Tech will make more than $2 million economic impact 3
(Date:7/7/2015)... ... July 07, 2015 , ... uBiome, the leading microbial genomics company, ... microbiome sampling kits and full 16S laboratory analysis. The goal is to fast-track a ... also have access to their own dashboard, enabling them to view and compare the ...
(Date:7/7/2015)... ... July 07, 2015 , ... Since its inception in ... What began as a conference for European Statistical Programmers, has evolved in the ... biometrical area. Statistical Programmers, Data Managers and Biostatisticians all over the world, share, ...
(Date:7/7/2015)... ... July 07, 2015 , ... ... Admin system was developed to solve many of the complex logistical and ... studies that involve multiple clinical sites and/or remote data coordination centers. , ...
(Date:7/6/2015)... , ... July 06, 2015 , ... ... fields holds potential to reveal how blood flows in the brain during stroke, ... the brain. , A new article published in the journal Neurophotonics ...
Breaking Biology Technology:uBiome to Award Grant of up to $100k for Groundbreaking Microbiome Research 2uBiome to Award Grant of up to $100k for Groundbreaking Microbiome Research 3PhUSE Delighted to Welcome Member Number 5.000 2ActiGraph Launches CentrePoint Cloud-based Study Management Software Platform 2ActiGraph Launches CentrePoint Cloud-based Study Management Software Platform 3Reducing Stroke Damage May be Next Application for OCT Technology Now Widely Used in Eye and Vision Healthcare 2Reducing Stroke Damage May be Next Application for OCT Technology Now Widely Used in Eye and Vision Healthcare 3