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Amylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)

ProductsAmylase Fluorometric (Green) Assay Kit, EnzChek® from Molecular Probes (Invitrogen)
Company Molecular Probes (Invitrogen)
Item Amylase Fluorometric (Green) Assay Kit, EnzChek®
Description a-Amylase is an enzyme that catalyzes the hydrolysis of starch to a mixture of maltose, maltotriose, and dextrins. The level of a-amylase in various fluids of the human body is of clinical significance in the diagnosis of disease states including pancreatitis and diabetes; plant and microbial a-amylases are important enzymes for industrial applications ranging from the manufacture of baked goods and dairy products to the production of ethanol and paper.14

The EnzChek Ultra Amylase Assay Kit (E33651) provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek kit contains a starch derivative the DQ starch substrate that is labeled with BODIPY FL dye (Figure 1) to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity (Figures 2 and 3) and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.

In tests using a-amylase from Bacillus sp. (Sigma A-6380) and 200 g/mL of the DQ starch substrate (30 minute incubation at room temperature), the EnzChek Ultra Amylase Assay Kit could be used to detect a-amylase activity down to a final concentration of 2 10-3 U/mL, where one unit is defined as the amount of enzyme required to liberate 1 mg of maltose from starch in 3 minutes at 20C, pH 6.9.

Info Molecular Probes (Invitrogen)Molecular Probes (Invitrogen)
PO Box 22010
Eugene, OR 97402-0469

Call Molecular Probes (Invitrogen) to buy products (US and Canada only; other locations use numbers below)
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Customer Service: 1-541-465-8300
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