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20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON

Products20S Proteasome Fluorometric (AMC) Assay Kit from CHEMICON
Item 20S Proteasome Fluorometric (AMC) Assay Kit
Price $325.00
Description The ubiquitin-proteasome pathway is the major proteolytic system in the cytosol of eukaryotic cells, where it catalyzes the selective degradation of short-lived regulatory proteins and the rapid elimination of proteins with abnormal conformation (Hershko & Ciechanover 1998; Hochstrasser 1996). The critical protease in this pathway is the 26S proteasome, an ATP-dependent proteolytic complex, which is formed by the association of the barrel-shaped 20S proteasome (700-kDa) and two 19S (700-kDa) regulatory complexes (Baumeister et al. 1998; Coux et al. 1996). The 20S Proteasome, catalytic core of the proteasome complex, is responsible for the breakdown of key proteins involved with apoptosis, DNA repair, endocytosis, and cell cycle control (Coux et al. 1996; Hoffman & Rechsteiner 1996; Hochstrasser 1995). The CHEMICON Proteasome Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of proteasome activity in cell lysates or inhibitor screening. Test Principle: Chemicons Proteasome Activity Assay Kit provides a simple and convenient means for assaying proteasome activity that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cleavage from the labeled substrate LLVY-AMC. The free AMC fluorescence can be quantified using a 380/460 nm filter set in a fluorometer. A proteasome inhibitor, Lactacystin, is included as a test inhibitor for screening purpose. Lactacystin is a microbial natural product and the most specific and potent inhibitor of proteasomes currently known (Fenteany & Schreiber 1998). Application: The Proteasome Activity Assay Kit is a relatively quick method for detection of intracellular proteasome activity. Testing of purified proteasome enzyme, in vitro inhibitor screening and the study of proteasome regulation can be performed with this assay.
CHEMICON International, Inc.
28820 Single Oak Drive
Temecula, CA 92590
Customer Service: 800-437-7500
Tech Support: 800-437-7500
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assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. Quantitation of phosphate content in protein, lipids, and DNA....
One-step, microplate or cuvet, colorimetric, detection range 0.1 mg/dL to 3 mg/dL. Procedure: 10 min....
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