Navigation Links
* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH

Products* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH
Company Gene Bridges GmbH
Item * Quick & Easy Conditional Knock Out Kit - FRT
Price 
Description This kit is designed for the fast and simple integration of FRT-sites into large vector plasmids at any position (for the generation of transgenic mouse models). The included FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid codes for an improved Flp recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype Flp enzyme at temperatures between 37C (optimal growth temperature for E.coli) and 40C (mouse body temperature). This kit can also be used to work on bacterial artificial chromosomes (BACs). The generation of targeting constructs is achieved utilizing the high Red/ET efficiency plus the convenient removal of the Red/ET Recombineering protein expression plasmid pRedET after recombination.
The kit is available to academic researchers. Commercial organizations require a sub-license from Gene Bridges.

  • Additional Specifications: Also available as as service.
Info Gene Bridges GmbH
Commercial Centre
Im Neuenheimer Feld 584
69120 Heidekberg, Germany
Customer Service: +49 (0)6221 13708 10
Fax Number: +49 (0)6221 13708 29
Web Site: http://www.Genebridges.com
NOTE: Price information is approximate list price and actual prices may vary.

Related biology products :

1. * Quick & Easy Conditional Knock Out Kit - loxP from Gene Bridges GmbH
2. QuickLane Individual Sample Sequencing from Agencourt Bioscience
3. * Quick & Easy E. coli Gene Deletion Kit from Gene Bridges GmbH
4. TNT® T7 Quick Coupled Transcription/Translation System from Promega
5. TargeTron Gene Knockout System from Sigma-Aldrich
6. FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
7. loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH
8. FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH
9. loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
10. FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
11. loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
... involved in translation initiation. PHAS-II is highest ... to eIF-4E, the mRNA cap-binding protein, and ... and in cells. Phosphorylation in the appropriate ... allows eIF-4E to bind to eIF-4G (p220), ...
MAb to HSV-2, gG-2 HSV-2 Glycoprotein G-2...
... unrelated proteins both of which function in ... in a p53-dependent manner. p14^ARF^ binds to ... mdm2 proteinrequired for mdm2 modification and concurrent ... is mediated by the exon 1Beta-encoded N-terminal ...
... contains a precipitant to ensure that the samples ... to allow the user to follow the progress ... ABgene ® s proprietary mixture of red ... composition used in ReddyRun™ molecular weight markers. It ...
Biology Products:
(Date:4/17/2017)... Florida , April 17, 2017 NXT-ID, ... technology company, announces the filing of its 2016 Annual Report on ... and Exchange Commission. ... on Form 10-K is available in the Investor Relations section of ... as on the SEC,s website at http://www.sec.gov . ...
(Date:4/11/2017)... Research and Markets has announced the addition ... their offering. ... tracking market to grow at a CAGR of 30.37% during the ... 2017-2021, has been prepared based on an in-depth market analysis with ... its growth prospects over the coming years. The report also includes ...
(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... a leading provider of patient support solutions, has announced the ... which will launch this week. The VMS CNEs will address ... enhance the patient care experience by delivering peer-to-peer education programs ... to help women who have been diagnosed and are being ... ...
(Date:10/11/2017)... ... October 11, 2017 , ... Singh ... orphan drug designation to SBT-100, its novel anti-STAT3 (Signal Transducer and Activator of ... SBT-100 is able to cross the cell membrane and bind intracellular STAT3 and ...
(Date:10/10/2017)... ... ... Dr. Bob Harman, founder and CEO of VetStem Biopharma, Inc. spent ... entitled “Stem Cells and Their Regenerative Powers,” was held on August 31st, 2017 ... joined by two human doctors: Peter B. Hanson, M.D., Chief of Orthopedic Surgery, Grossmont ...
(Date:10/10/2017)... ... October 10, 2017 , ... The Pittcon Program Committee is ... honoring scientists who have made outstanding contributions to analytical chemistry and applied spectroscopy. ... world’s leading conference and exposition for laboratory science, which will be held February ...
Breaking Biology Technology: