Navigation Links
Used in a new way, RNA interference permanently silences key breast cancer gene

In laboratory mouse experiments, researchers at The University of Texas M. D. Anderson Cancer Center have developed a way to use RNA interference (RNAi) so that it permanently hampers breast cancer development. The technique permanently silences activated STAT3, a crucial gene found in some human breast tumors, thus reducing the cancer's ability to become invasive.

The study, presented at the annual meeting of the American Association for Cancer Research (AACR), used a modified form of RNAi to silence STAT3 in a permanent way. Typically, only a transient effect is achieved with RNAi before the tiny bits of genetic material are become inactive as the cell population continues to expand.

"We are a long way from using this technique in patients, but this study shows that that it may be possible to use RNAi in more than just experiments that silence genes temporarily," says the study's principal investigator, Ralph Arlinghaus, Ph.D., a professor and chair of the Department of Molecular Pathology. Details of the study appeared in the April 1 2005 issue of the journal Cancer Research.

"The technique is also providing some valuable insights into the role of STAT3 and its downstream targets," adds Arlinghaus, who also will discuss the work in a mini symposium at the AACR meeting.

RNAi has been employed as a laboratory tool to knock down expression of genes in a variety of cells and organisms. It works by introducing a small double-stranded RNA (RNAi) that specifically targets a gene's product, its messenger RNA. This action then blocks translation and production of the protein that the gene encodes.

In this study, the researchers used a lentivirus (a type of retrovirus) to deliver a specifically designed long-acting small interfering RNA (termed a short hairpin RNA, shRNA) for mouse STAT3 into a mouse breast cancer cell line. They chose STAT3 because when activated, it is involved in the formation of multiple types of tumors, including
'"/>

Source:University of Texas M. D. Anderson Cancer Center


Page: 1 2

Related biology news :

1. Scientists identify genetic pathways essential to RNA interference
2. Silence the gene, save the cell: RNA interference as promising therapy for ALS
3. Scientists identify genetic pathways essential to RNA interference
4. RNA interference stops colon cancer spread in mice
5. Cocaine high caused by interference in neuronal receiving stations
6. Poor prenatal nutrition permanently damages function of insulin-producing cells in the pancreas
7. Interfering RNA silences genes in slippery immune cells
8. Researchers find protein that silences genes
9. MIT: Pulsing light silences overactive neurons
10. Viral DNA sequence a possible trigger for breast cancer
11. Biomarkers isolated from saliva successfully predict oral and breast cancer
Post Your Comments:
(Date:5/19/2013)... that can grow using hydrogen gas as its sole ... of carbon. Researchers at the University of Massachusetts, ... of the American Society for Microbiology. , "This represents ... says Amit Kumar, a researcher on the study who, ... Lab Group at the university. , Under the leadership ...
(Date:5/18/2013)... The AGA Research Foundation is honored to announce ... in Digestive Cancer, which will support Andrew D. Rhim, ... University of Pennsylvania, Philadelphia, as he furthers his research ... and cancer progression. , "The AGA Research Foundation is ... distinguished clinicians and AGA Legacy Society members," said Nicholas ...
(Date:5/18/2013)... 18, 2013) Research presented at Digestive Disease ... health through diet and lifestyle. , Individuals ... reduced muscle strength, fatigue and poor quality of ... are in remission. A randomized, double blind, placebo-controlled ... D supplementation corresponded to significant relief of these ...
Breaking Biology News(10 mins):AGA Research Foundation grant furthers digestive cancer research 2Research examines new methods for managing digestive health 2Research examines new methods for managing digestive health 3
... research shows that birds and other animals change their behavior ... traffic or the hum of machinery. But human clamor doesn,t ... eat or disperse their seeds, human noise can have ripple ... cases where noise has ripple effects on long-lived plants like ...
... Mich. In a surprising result, Michigan State University ... disease found that mice on a calorie-restricted diet were ... inflammation-causing bacterial pathogen in the colon. While ... inflammatory bowel diseases such as colitis, the study results ...
... CAMBRIDGE, MD (March 20, 2012)Since the explosion on the BP ... April 20, 2010, scientists have been working to understand the ... months, crude oil gushed into the water at a rate ... capped on July 15, 2010. A new study confirms that ...
Cached Biology News:Not just for the birds: Man-made noise has ripple effects on plants, too 2Not just for the birds: Man-made noise has ripple effects on plants, too 3Low-calorie diet may be harmful for bowel disease patients 2Study confirms oil from Deepwater Horizon disaster entered food chain in the Gulf of Mexico 2
(Date:5/21/2013)... Alpha Source, Inc., a Wisconsin-based ... for the 13th consecutive year at the Association ... Conference and Expo. The conference brings together professionals ... technologies. This year’s event will be held from ... The three-day event draws over 1,500 healthcare technology ...
(Date:5/21/2013)... 2013 Smarter Security, Inc., provider of ... announced that Genzyme, a Sanofi company and leading biotech ... CL for a multi-use facility in the United States. ... Genzyme buildings, this latest order underscores the satisfaction and ... detection device improving doorway access control. , ...
(Date:5/21/2013)... May 21, 2013 Emerson Resources ... that Dr. Charles Signorino, the Company’s Chief Executive Officer ... 2013 after 32 years of service. As part of ... current President, has been named as Dr. Signorino’s successor. ... the pharmaceutical industry, having made a significant contribution to ...
(Date:5/21/2013)... Bed bugs have become so widespread in the past ... to chemical-based pesticides for help to completely get rid of ... solutions present, the company advised the public to kill ... According to My Cleaning Products, chemical-based pesticides are not as ... shared that the pests have already developed immunity to the ...
Breaking Biology Technology:Alpha Source, Inc. to Exhibit at the AAMI 2013 Conference and Expo 2Door Security at Genzyme, a Sanofi Company, Enhanced with Door Detective from Smarter Security 2Door Security at Genzyme, a Sanofi Company, Enhanced with Door Detective from Smarter Security 3Emerson Resources Announces CEO Retirement and Appointment of Successor 2Emerson Resources Announces CEO Retirement and Appointment of Successor 3New Guide to Kill Bed Bugs Published by My Cleaning Products, Company Enumerates Non-Toxic Means to Clear the Pests 2New Guide to Kill Bed Bugs Published by My Cleaning Products, Company Enumerates Non-Toxic Means to Clear the Pests 3
... Muscular Dystrophy Association announced today that it has awarded ... to develop and test a "dissociative glucocorticoid" as a ... (Logo: http://photos.prnewswire.com/prnh/20120424/DC93207LOGO ) ... Health Therapeutics for Rare and Neglected Diseases (TRND) program ...
... ZyGEM Corp. Ltd., a developer and marketer of ... nucleic acids, today announced it has entered into a ... DNA and RNA extraction products in Sweden and other ... of quantitative real-time PCR (qPCR) services and products.  This ...
... 3, 2012 CMC Biologics, a leading contract manufacturing ... in process development and cGMP manufacture , has ... of the Year – Large Category.   The Seattle Business ... Impact Washington, brought together nearly 300 leaders in manufacturing ...
Cached Biology Technology:MDA Supports Development of 'Dissociative Glucocorticoid' for DMD 2MDA Supports Development of 'Dissociative Glucocorticoid' for DMD 3ZyGEM Teams With TATAA Biocenter To Distribute Its Innovative Nucleic acid Extraction Kits 2ZyGEM Teams With TATAA Biocenter To Distribute Its Innovative Nucleic acid Extraction Kits 3CMC Biologics Honored as Finalist for Washington's Manufacturing Company of the Year Award 2
... generation of microarrays to detect alternative splicing. ... rapidly elucidate alternatively spliced events in gene ... discovery. The service combines ExonHits proprietary splice ... analysis with Agilents robust microarray platform. The ...
... CE 4100 high pressure pump with ... with software drive compensation, reducing pulsing to ... selectable in 0.001mL steps. , The pump ... bio-compatible., Pumps may be programmed from their ...
... III variable angle system, 220-240 V, is used ... Mb DNA fragments by pulsed field gel electrophoresis ... module, variable-speed pump, 14 x 13 cm casting ... 1.5 mm thick comb, screened cap, disposable plug ...
... PVDF membrane and filter paper sandwiches are ... and two sheets of thick filter paper ... and filter paper sandwich. These 7 x ... capacity immunoblotting fit Ready Gel-sized gels and ...
Biology Products: