Navigation Links
Tagging pathogens with synthetic DNA 'barcodes'

NA that carries the genetic code in living cells consists of two complementary strands that attach to one another along their length. But Luo's research purposely and completely ignores the DNA's genetic coding properties. He uses DNA, he said, as a "generic instead of a genetic material."

By synthesizing three short strands of DNA, each of which is complementary to one of the others along half its length, the researchers can create a Y-shaped structure. Combining several of these structures creates a web with many branching ends. "While DNA is flexible, the short strands used here are quite rigid," Luo said. "A long piece of spaghetti is floppy, but a short bit of it is quite stiff."

An antibody or some other molecule that will bind to the molecule to be detected is attached to one of the loose ends of the DNA. To other ends are attached molecules of fluorescent dye in a predetermined pattern.

For example, one probe might contain four molecules of green dye and one of red. Another might have three molecules of green and two of red, and so on. If a mixture of several probes is added to a solution containing, for example, E. coli bacterial DNA, only probes with a particular color code will be programmed to bind to that DNA. The results can be seen under a fluorescent light microscope using colored filters that pass only one color at a time. A signal in which the ratio of intensity of green light is four times that of red light, for example, identifies a "4G1R" probe. The researchers say that up to 1,000 different codes can be created using only three fluorescent dyes.

To amplify the signals, the researchers attached many DNA probes to the surface of polystyrene microbeads 5.5 microns (millionths of a meter) in diameter. The results can be read in several ways. One is in a flow cytometer, in which samples move rapidly past a window where a computer reads the color codes of individual beads. Another is by dot blotting, in which the sampl
'"/>

Source:Cornell University News Service


Page: 1 2 3

Related biology news :

1. A new study examines how shared pathogens affect host populations
2. New lab technique identifies high levels of pathogens in therapy pool
3. Edible bivalves as a source of human pathogens: signals between vibrios and the bivalve host.
4. Study: Plants use dual defense system to fight pathogens
5. CO2 sensing proves critical for fungal pathogens to adapt to life in air and human hosts
6. How Rickettsial pathogens break into cells
7. Ticks, flukes, and genomics: Emerging pathogens revealed
8. Gene thwarts some pathogens, gives access to others, could save crops
9. Researchers map spread of pathogens in the human body
10. Researchers develop technologies to devour food pathogens
11. GreeneChip -- New diagnostic tool that rapidly and accurately identifies multiple pathogens
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Tagging pathogens with synthetic DNA barcodes

(Date:9/2/2014)... new Journal of Evolutionary Biology study ... can influence language diversification. , Investigators argue that ... biodiversity in the Galpagos Islands is also responsible ... the natural oceanic barriers that impede interaction between ... from one another due to a reduction of ...
(Date:9/2/2014)... that professional baseball pitchers with poor core stability are more ... season because of injury than are pitchers who have good ... In the study, 347 pitchers were assessed for lumbopelvic control ... as they raised a leg to step up were up ... days cumulative, not consecutive during the season than ...
(Date:9/2/2014)... is considered the most ideal source of nutrition ... critical role in the evolution and civilizations of ... contains a large number of bacterial species, including ... as no surprise to scientists and physicians. , ... to be the result of co-evolutionary and co-adaptive ...
Breaking Biology News(10 mins):In pro baseball pitchers, weak core linked to more missed days 2In pro baseball pitchers, weak core linked to more missed days 3Are human breast milk microbiome 'neutral'? 2Are human breast milk microbiome 'neutral'? 3
... of 1000, the leading scientific evaluation service, has highlighted research ... Birds use plumage colour to recognize and select potential mates. ... a striking colour change, as demonstrated by two closely related ... in the American Naturalist -- selected and reviewed ...
... the possibility that flies and other insects that encounter nanomaterial ... could pick up and transport nanoparticles on their bodies, transferring ... The study on carbon nanoparticles barely 1/5,000th the width ... issue of ACS, Environmental Science & Technology , a ...
... an advance that could help ease the antibiotic drought, ... test that enlists pinhead-sized worms in efforts to discover ... ACS Chemical Biology , a monthly journal. ... that existing methods for identifying germ-fighting drugs involve adding ...
Cached Biology News:
(Date:9/2/2014)... (PRWEB) September 02, 2014 The Little ... southwestern Chicagoland, constructed a new 290,000 square-foot West Pavilion ... Tufftec® Lockers from Scranton Products for the nurse’s ... 1930, Little Company of Mary Hospital’s $180 ... Summer 2014 with the connection of the West Pavilion ...
(Date:9/2/2014)... a billionth of a meter in size, are around ... benefit human health, as in some innovative early cancer ... viruses, air pollution, traffic emissions, cosmetics, sunscreen and electronics. ... St. Louis, led by Lan Yang, PhD, the Das ... Engineering, and their collaborators at Tsinghua University in China ...
(Date:9/2/2014)... YORK , September 2, 2014 ... Study on Membrane Technology in Pharmaceutical, Biopharma and Life ... Highest Growth by 2019" the global membrane technology market for pharmaceutical, ... million in 2014 and is expected to grow at ... reach an estimated value of USD 10,886.0 million in ...
(Date:9/2/2014)... 2014 Opertech Bio, Inc., a ... cost effective approach to sensory evaluation, today announced ... the U.S. Patent and Trademark Office, encompassing high-throughput ... , “Opertech’s taste evaluation technology called Microtiter Operant ... throughput system for rapid characterization of taste sensory ...
Breaking Biology Technology:Tufftec® Lockers from Scranton Products Selected by Little Company of Mary Hospital Near Chicago to Create an Uplifting Environment 2Tufftec® Lockers from Scranton Products Selected by Little Company of Mary Hospital Near Chicago to Create an Uplifting Environment 3Engineers develop new sensor to detect tiny individual nanoparticles 2Engineers develop new sensor to detect tiny individual nanoparticles 3Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 2Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 3Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 4Global Membrane Technology Market Will Reach $10,886.0 Million in 2019: Persistence Market Research 5Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2
... , QUEBEC CITY , Jan. 22 ... TSX: AEZ) (the "Company"), a late-stage drug development company specialized ... 21, 2010 , the Company received a letter from the ... the minimum closing bid price of its common shares had ...
... , ... Bioamber,( http://www.bio-amber.com/ ) , a joint venture between US-based DNP,( ... )(Agro-industrie Recherches et Développements),announces the successful start-up and commissioning of ... , the plant has been,producing renewable succinic acid from wheat ...
... ... contouring, will be presented at the upcoming ICAD & 3rd ECAA Asia conference. Dr. ... Y. Yu will present his latest clinical data showing successful reduction of acne using TriPollar. ... (PRWEB) January 20, ...
Cached Biology Technology:Æterna Zentaris Receives NASDAQ Notification Related to Minimum Bid Price 2Æterna Zentaris Receives NASDAQ Notification Related to Minimum Bid Price 3Bioamber Commissions World's First Renewable Succinic Acid Plant 2Bioamber Commissions World's First Renewable Succinic Acid Plant 3TriPollar Technology Clinically Proven to Significantly Reduce Stretch Marks and Acne 2TriPollar Technology Clinically Proven to Significantly Reduce Stretch Marks and Acne 3