Navigation Links
Study reveals new technique for fingerprinting environmental samples

Groundbreaking research led by the U.S. Department of Energy Joint Genome Institute (DOE JGI) demonstrates for the first time that the signatures of the genes alone in terrestrial and aquatic samples can accurately diagnose the health of the sampled environments. This study, published in the April 22nd edition of the journal Science positions large-scale genome sequencing to accelerate advances in environmental sciences akin to the contributions DNA sequencing has made to biomedical sciences.

"These DNA sequence fingerprints can be used to provide highly accurate assessments of the vitality of extremely diverse environments," said Dr. Raymond L. Orbach, Director of the DOE Office of Science, which supported the research. "These fingerprints can be used to reveal environments under stress as well as signal progress in remediating contaminated environments. This may well develop environmental ecology into a fully quantitative science."

Dubbed Environmental Genomic Tags, or EGTs, these indicators capture a DNA profile of a particular niche and reflect the presence and levels of nutrients, pollutants, and other environmental features.

The EGT approach employed in the study shares similarities with aspects of the Human Genome Project research. In the early 1990s, incomplete fragments of human genes called Expressed Sequence Tags (ESTs) were used as diagnostic fingerprints for human tissues to determine their unique features and disease status. These information-rich data allowed researchers to forge ahead with studying genes important in disease processes, long before the completion of the entire human genome.

"EGT fingerprints may be able to offer fundamental insights into the factors impacting on various environments," said DOE JGI Director, Eddy Rubin, who led the research team. "With EGTs we don't actually need a complete genome's worth of data to understand the functions required of the organisms living in a particular setting. Rather, the genes present and their abundances in the EGT data reflect the demands of the setting and, accordingly, can tell us about what's happening in an environment without knowing the identities of the microbes living there."

The EGT fingerprints capture a DNA profile of a particular niche and reflect the presence and levels of nutrients, and pollutants, as well as features like the light and temperature. For example, genes involved in breaking down plant material are over represented in soil and absent in the sea water, while in sea water, genes involved in the passage of sodium, a major chemical component of salt water, were particularly abundant. As light is a major energy source for microbes living in surface water there was an abundance of genes involved in photosynthesis in samples collected from shallow water. These differences in the abundances of genes involved in particular functions provide DNA clues to features of the environments from where the samples were taken. Importantly the DNA clues were easy to find despite the vast numbers of different individual microbial species within the samples.

Rubin argues that the genes and their relative abundance as gleaned from EGTs reflect the intricate physical and biochemical details of a given environment without having to know the actual identities of the resident microbes. This finding is crucial for studies of microbes in the wild since the sheer number of different organisms present in nearly all environments makes it a daunting task to sequence the multitude of organisms one at a time. With the EGT approach, an abbreviated sequencing effort enables scientists to piece together the information and form a useful metabolic picture of an entire complex environment.

This study was done in collaboration with Diversa Corporation of San Diego, Calif. who provided the DNA from the different environments which included soil from a Minnesota farm and samples of several different whale skeletons co llected from the Pacific and Artic ocean depths separated by thousands of miles. The DNA was then sequenced and analyzed by the JGI. While different environments present different metabolic pictures, this research, Rubin said, facilitates a holistic perspective on environmental systems, something that many scientists will welcome.

"Environmental systems are extremely complex, harboring numerous diverse species coexisting in a single locale," said Rubin. "By focusing on the information encoded in the DNA fragments sequenced, independent of the organisms from which they derived, we were able to get around the problem of species diversity."

Very little is known about the microbes since the vast majority of them, some 99 percent, are resistant to being grown under standard laboratory conditions. The EGT strategy's particular utility is that it offers an easily accessible window into that important part of the biosphere with significant impact on the environment.

DOE JGI postdoctoral fellow Susannah Green Tringe was first author on the Science paper that, in addition to other DOE JGI scientists, included collaborators from Diversa Corporation, and the European Molecular Biology Laboratory, Heidelberg, Germany.


'"/>

Source:DOE/Joint Genome Institute


Related biology news :

1. Novel Asthma Study Shows Multiple Genetic Input Required; Single-gene Solution Shot Down
2. Emory Study Tests Bone Marrow Stem Cells to Improve Circulation in Legs
3. UCLA Study Shows One-Third of Drug Ads in Medical Journals Do Not Contain References Supporting Medical Claims
4. Study Demonstrates Gene Expression Microarrays are Comparable and Reproducible
5. Study Links Ebola Outbreaks To Animal Carcasses
6. Breakthrough Microarray-based Technology for the Study of Cancer
7. NYU Study Reveals How Brains Immune System Fights Viral Encephalitis
8. Study finds more than one-third of human genome regulated by RNA
9. Leukemia Drug Breakthrough Study In New England Journal Of Medicine
10. Study identifies predictors of HIV drug resistance in patients beginning triple therapy
11. New Study from Affymetrix Laboratories Points to Changing View of How Genome Works
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/23/2017)... , Mar. 23, 2017 Research and ... System Market Analysis & Trends - Industry Forecast to 2025" ... ... grow at a CAGR of around 8.8% over the next decade ... industry report analyzes the market estimates and forecasts for all the ...
(Date:3/22/2017)... March 21, 2017 Optimove , ... by retailers such as 1-800-Flowers and AdoreMe, today ... Recommendations and Replenishment. Using Optimove,s machine learning algorithms, ... product and replenishment recommendations to their customers based ... predictions of customer intent drawn from a complex ...
(Date:3/20/2017)... At this year,s CeBIT Chancellor Dr. Angela Merkel ... came to the DERMALOG stand together with the Japanese Prime Minster Shinzo ... At the largest German biometrics company the two government leaders could see ... as well as DERMALOG´s multi-biometrics system.   Continue ... ...
Breaking Biology News(10 mins):
(Date:8/16/2017)... ... 16, 2017 , ... While art and science are often thought of as ... might think. A Mesh Is Also a Snare, a group exhibition presented by ... Esther Klein Gallery (EKG) on August 17 and run through September 30. An opening ...
(Date:8/16/2017)... , Aug. 16, 2017  Kingfisher Talent, the ... leadership development, and Virdis Group, global executive search specialists in ... that enables clients to leverage the expertise and reach of ... clients here in the Boston biotech ... of leadership talent throughout the US, Canada ...
(Date:8/15/2017)... ... August 15, 2017 , ... Pittcon is pleased to once ... scientific instruments. This year’s symposium, organized by the Pittcon 2018 program chair, Annette ... Applications.” This dynamic presentation will discuss novel ionization processes, high throughput IMS-MS technologies, ...
(Date:8/15/2017)... ... August 15, 2017 , ... Coffea ... various biotic and abiotic factors. During this educational webinar, participants will learn about ... well as gain a better understanding of how genomics is important for coffee ...
Breaking Biology Technology: