Navigation Links
Sea coral's trick helps scientists tag proteins

The glow emitted by a variety of sea coral helped Russian scientists harness the protein that generates the light to create a tiny fluorescent tag that responds to visible light. The two-color tag should help researchers follow individual proteins as they dart around inside living cells.

Under a microscope, the two-color tag--called Dendra because it is derived from the sea coral Dendronephthya--first shows up as a green glow, highlighting the otherwise invisible protein to which it is attached. The green turns to red when the tags are zapped with an intense pulse of visible blue light.

The dramatic color change "makes it possible to precisely label an object, such as a cell, organelle, or protein, with a flash of light and then to follow the object's movement over time," said Konstantin Lukyanov, whose group conducted the Dendra research in the lab of his brother, Sergey Lukyanov, a Howard Hughes Medical Institute (HHMI) international research scholar at the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences in Moscow. "This tool opens new possibilities for studying protein and organelle dynamics in living cells, cell migration during embryogenesis, inflammation, and other pathological and normal processes," he said.

Unlike other green-to-red fluorescent markers, Dendra can be activated by visible blue light, which is less harmful to living cells and does not require special laser equipment, Konstantin Lukyanov explained. In fact, the light source of the laser-scanning confocal microscopes that researchers commonly use to peer into living cells can activate the tag, which should make the new tool useful to many scientists, said Sergey Lukyanov, whose lab reports on the Dendra tag in the April 2006 issue of Nature Biotechnology, published in advance online on March 19, 2006.

Importantly, Dendra is small enough to highlight proteins without interfering with their folding or function. And the resea
'"/>

Source:Howard Hughes Medical Institute


Page: 1 2 3

Related biology news :

1. Old drug, new tricks: Prospects for slashing the impact of malaria
2. Dangerous tricksters: Some bacterie use immune cells to reproduce
3. Researchers use trickery to create immune response against melanoma
4. New finding points way to foiling anthraxs tricks
5. A wolf in sheeps clothing: Plague bacteria reveal one of their virulence tricks
6. An AIDS-related virus tricks cells to become tumors, new Penn study finds
7. Scientists perform tricky operation
8. Yale biologists trick viruses into extinction
9. Toxoplasmosis infection trick revealed by scientists
10. Jumping gene helps explain immune systems abilities
11. Protein helps regulate the genes of embryonic stem cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Sea coral trick helps scientists tag proteins

(Date:11/6/2014)... cancer deaths occur because of metastasis, yet progress ... been slow. , "It,s been particularly challenging to ... Gujral, research fellow in systems biology at Harvard ... detected until after they,ve already metastasized." , Gujral ... that should help researchers better understand how metastasis ...
(Date:11/5/2014)... career to understanding the Earth,s climate and communicating ... deputy director and regional climatologist for the Western ... Redmond has more than three decades of experience ... data to the general public. , At its ... December 15-19, 2014 the American Geophysical Union (AGU) ...
(Date:11/4/2014)... Temple University School of Medicine announced today that ... initiative funded by the Bill & Melinda Gates ... Obstetrics, Gynecology and Reproductive Sciences; and Vice Chair ... School of Medicine will pursue an innovative global ... Biomarkers to Assess Fetal Neurodevelopment.", Grand Challenges Explorations ...
Breaking Biology News(10 mins):Migration negation 2Migration negation 3Nevada climatologist to be honored at AGU Fall Meeting for lifetime of public outreach 2Temple University School of Medicine receives Grand Challenges Explorations grant 2
... DAVIS--Mosquito traps that reek like latrines may be no ... team led by chemical ecologist Walter Leal has discovered ... making humans hold their noses. The synthetic mixture, ... just as enticing to Culex mosquitoes as the current ...
... and South Africa have created a biophysical methodology ... potentially remedy even substantial hearing loss. The authors ... the ear to recognize frequencies originally associated with ... the open-access journal PLoS Computational Biology . ...
... Presenting new research about national, home and business security ... of Houston professor and his colleagues has become the ... to surveillance. Two professors and two ... Institute of Electrical and Electronics Engineers International Conference on ...
Cached Biology News:No more big stink: scent lures mosquitoes, but humans can't smell it 2Biophysical method may help to recover hearing 2National security remedies among topics at surveillance confab 2National security remedies among topics at surveillance confab 3
(Date:11/18/2014)... RPS Diagnostics (RPS®) – a developer ... its third annual partnership of the Centers for ... Antibiotics Week from November 17-23. , Get Smart ... to highlight the coordinated efforts of CDC, state ... partners to provide education about the importance of ...
(Date:11/18/2014)... (PRWEB) November 18, 2014 Alanda ... and Transparency Reporting solutions, announced today that a ... using its Consummate Providerâ„¢ solution in both the ... a Global, SaaS based, Data Quality remediation portal, ... their third party Providers improve their ability to ...
(Date:11/18/2014)... 2014 Array Architects is proud of ... to the nation's hospitals and health systems. It is ... dedicating their careers to the advancement of both the ... her respective field, Laura Silvoy has been chosen to ... Winter Simulation Conference. , Laura will present her ...
(Date:11/18/2014)... DOYLESTOWN, Pa. , Nov. 18, 2014  Novira ... therapies for curative treatment of chronic hepatitis B virus ... , M.D., was appointed to its Scientific Advisory Board ... Brian join our SAB," said Christian S. Schade ... wealth of clinical development experience which will be invaluable ...
Breaking Biology Technology:RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 3Alanda Software Completes Successful Implementation of Global Data Quality & Vendor Management Solution for Top Tier Global Biopharmaceutical Company 2Array Healthcare Simulation Expert Slated to Speak at Winter Simulation Conference 2Novira Therapeutics Announces Appointment of Brian F. Daniels, M.D., to its Scientific Advisory Board 2Novira Therapeutics Announces Appointment of Brian F. Daniels, M.D., to its Scientific Advisory Board 3
... Feb. 10 Microdosing or Phase 0 clinical ... an imperative phase of the drug development process ... niche capability providers in preclinical testing and technology ... partnerships and alliances, fuelling growth opportunities in this ...
... an MBA-student organized event, will be held February 19-20 in Philadelphia. ... from Healthcare Services, Investment, Pharma, Biotech, and Medical Device sectors. ... ... 2009 Wharton Health Care Business Conference , an MBA-student organized ...
... DSM Biologics and Crucell N.V. (Euronext, Nasdaq: ... they have entered into an agreement with Bioceros B.V. ... Under the terms of the agreement, Bioceros will be ... the PER.C6(R) cell line located in the European Union.Karen ...
Cached Biology Technology:Frost & Sullivan Sees Microdosing as Compelling Advantage for Big Pharma 2Frost & Sullivan Sees Microdosing as Compelling Advantage for Big Pharma 314th Annual Wharton Health Care Business Conference to Feature CEOs from WebMD, McKesson, Humana and Given Imaging 2DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 2DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 3DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 4DSM and Crucell Sign Agreement with Bioceros to Serve as Pre-Approved Cell Line Generation Partner of PER.C6(R) Cell Line Proteins 5
Request Info...
... 5B15-5B-11E. Immunogen: Synthetic peptide derived from ... protein. Specificity: Specific for the ... Western blots it identifies the target band ... (positive controls: deglycosylated secreted media from primary ...
Lipid-rich bovine serum albumin for cell culture. It is chromatographically purified and has an IgG content 1.0% (w/w)....
Prepared with distilled water. Yeast extract solution is autolyzed at 150 g/L....
Biology Products: