Navigation Links
Scientists develop new color-coded test for protein folding

Every protein--from albumin to testosterone (Ed : testosterone isn't a protein, it's a steroid)--is folded into a unique, three-dimensional shape that allows it to function properly. Now Stanford University scientists have developed a simple test that instantly changes color when a protein molecule attached to a gold nanoparticle folds or unfolds. The new technique, which works on the same principle as ordinary pH tests that measure the acidity of water, is described in the March 2005 issue of the journal Chemistry and Biology.

"What we've developed is a simple and inexpensive sensor for determining when a protein changes its conformation," said study co-author Richard N. Zare, the Marguerite Blake Wilbur Professor in Natural Science in Stanford's Department of Chemistry. According to Zare, the new sensor may eventually provide biomedical researchers a fast, affordable method for detecting antibodies and other disease-related proteins. Acid and base

In their experiment, Zare, postdoctoral fellow Soonwoo Chah and graduate student Matthew R. Hammond created a liquid solution containing nano-sized particles of gold saturated with a protein called cytochrome c.

"We chose gold nanoparticles because they are simple to prepare, easy to control and cost effective," the authors wrote. "To the best of our knowledge, however, gold nanoparticles have not been previously used to investigate the folding and unfolding of proteins."

The initial batch of gold-cytochrome solution had a rosy red hue and a pH value of 10--about the same as an over-the-counter heartburn medication. But when drops of hydrochloric acid were added, the solution began to change color, turning purple when the pH reached 5.8 and light blue at pH 4, which is close to the acidity of wine. Lab analysis revealed that additional hydrochloric acid was causing the cytochrome c molecules to unfold. As a result, gold nanoparticles coated with cytochrome c began clumping together
'"/>

Source:Stanford University


Page: 1 2

Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists identify genetic pathways essential to RNA interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Scientists develop new color coded test for protein folding

(Date:2/10/2015)... WALTHAM, Mass., Feb. 10, 2015  Alere Inc. ... announced its financial results for the quarter ended ... Executive Officer and President of Alere said, "We ... refocussing Alere as the global leader in rapid ... closing the Alere Health divestiture in early January ...
(Date:2/5/2015)... 5, 2015  Marken is starting its 35 th ... launched a new marketing campaign to solidify its place ... new campaign focuses on First as a ... The first headline in the series, ... with its client,s priorities. Marken recognizes the need to ...
(Date:2/5/2015)... Calif. , Jan. 27, 2015 /PRNewswire/ ... provider of globally deployed, innovative test solutions ... announced the delivery of its TS-323 ... Space Systems Company (LMSSC). GENASYS is a ... demands of mission-critical applications that require performance ...
Breaking Biology News(10 mins):Alere Inc. Announces Fourth Quarter 2014 Results 2Alere Inc. Announces Fourth Quarter 2014 Results 3Alere Inc. Announces Fourth Quarter 2014 Results 4Alere Inc. Announces Fourth Quarter 2014 Results 5Alere Inc. Announces Fourth Quarter 2014 Results 6Alere Inc. Announces Fourth Quarter 2014 Results 7Alere Inc. Announces Fourth Quarter 2014 Results 8Alere Inc. Announces Fourth Quarter 2014 Results 9Alere Inc. Announces Fourth Quarter 2014 Results 10Alere Inc. Announces Fourth Quarter 2014 Results 11Alere Inc. Announces Fourth Quarter 2014 Results 12Alere Inc. Announces Fourth Quarter 2014 Results 13Alere Inc. Announces Fourth Quarter 2014 Results 14Alere Inc. Announces Fourth Quarter 2014 Results 15Alere Inc. Announces Fourth Quarter 2014 Results 16Alere Inc. Announces Fourth Quarter 2014 Results 17Alere Inc. Announces Fourth Quarter 2014 Results 18Alere Inc. Announces Fourth Quarter 2014 Results 19Alere Inc. Announces Fourth Quarter 2014 Results 20Alere Inc. Announces Fourth Quarter 2014 Results 21Alere Inc. Announces Fourth Quarter 2014 Results 22Alere Inc. Announces Fourth Quarter 2014 Results 23Alere Inc. Announces Fourth Quarter 2014 Results 24Alere Inc. Announces Fourth Quarter 2014 Results 25Alere Inc. Announces Fourth Quarter 2014 Results 26Alere Inc. Announces Fourth Quarter 2014 Results 27Alere Inc. Announces Fourth Quarter 2014 Results 28Alere Inc. Announces Fourth Quarter 2014 Results 29Alere Inc. Announces Fourth Quarter 2014 Results 30Alere Inc. Announces Fourth Quarter 2014 Results 31Alere Inc. Announces Fourth Quarter 2014 Results 32Alere Inc. Announces Fourth Quarter 2014 Results 33Alere Inc. Announces Fourth Quarter 2014 Results 34Alere Inc. Announces Fourth Quarter 2014 Results 35Alere Inc. Announces Fourth Quarter 2014 Results 36
... for cancer therapy has been identified by Monash University ... a gene involved in cancer development. , A team ... of Immunology, has identified two proteins that are involved ... telomerase that is essential if cancer cells are to ...
... accumulation of genetic damage in our cells is a major ... published today in the journal Nature by an international group ... critical gene for repairing damaged DNA grow old rapidly and ... that grow old naturally. Furthermore, the premature aging symptoms of ...
... (NRL) and Purdue University have shed light on one ... magnetic. Their description of how being magnetic "helps" ... of the Biophysical Journal. , Magnetic bacteria are found ... and lakes. The strain of bacterium the research ...
Cached Biology News:New target for cancer therapy identified 2Buildup of damaged DNA in cells drives aging 2Buildup of damaged DNA in cells drives aging 3Buildup of damaged DNA in cells drives aging 4Researchers reveal mystery of bacterial magnetism 2
(Date:2/26/2015)... CA (PRWEB) February 26, 2015 The ... online demonstration to support life extension technologies and awareness. ... UTC, Universal Coordinated Time, on March 21st 2015, in ... world. , Eric Schulke, Founder of MILE, says, ... act with urgency. The Movement for Indefinite Life Extension ...
(Date:2/26/2015)... Pittsburgh, PA (PRWEB) February 26, 2015 ... March 9 -12, at the Ernest N. Morial ... 919 exhibitors (count as of February 19, 2015) ... community in industrial, academic, and government labs. The ... such as, but not limited to, analytical chemistry; ...
(Date:2/26/2015)... JUPITER, Fla. , Feb. 26, 2015 /PRNewswire/ ... biotechnology company whose patented and proprietary technologies are ... other proteins for the bioenergy, bio-based chemical, biopharmaceutical ... will report its financial results for year ending ... March 11, 2015 and will host a conference ...
(Date:2/26/2015)... 26, 2015   Lexicon Pharmaceuticals, Inc. (Nasdaq: ... at the Cowen 35 th Annual Health Care Conference ... Boston .  Lexicon management will provide an ... A webcast of the presentation will be available through Lexicon,s ... presentation will be available at www.lexpharma.com until April ...
Breaking Biology Technology:Indefinite Life Extension Activists Organize Online Demonstration 2Indefinite Life Extension Activists Organize Online Demonstration 3Pittcon 2015 Announces Exposition Highlights 2Dyadic International To Announce Fourth Quarter 2014 Financial Results And Host Conference Call On Wednesday, March 11, 2015 2Dyadic International To Announce Fourth Quarter 2014 Financial Results And Host Conference Call On Wednesday, March 11, 2015 3Lexicon To Present At The Cowen 35th Annual Health Care Conference 2
... Boston Scientific Corporation (NYSE: BSX ) ... and news announcements at the 30th Annual Scientific Sessions ... 13 to 16 in Boston. Highlights of the ... II clinical trial and the ALTITUDE Clinical Science Program. ...
... packaging equipment manufacturer, Sepha Ltd, enters strategic European alliances and gears ... Achema 2009 , ... Belfast (Vocus) May 11, 2009 ... and de-blistering equipment, Sepha Ltd is boosting its ...
... Pa., May 8 BioTrends Research Group released highlights from ... a four wave syndicated research study that will track uptake ... launch. The research is based on results of a ... May 7th. 95% of the Rheumatologists surveyed were aware ...
Cached Biology Technology:Boston Scientific Announces Company Schedule for Heart Rhythm Society Scientific Sessions 2Boston Scientific Announces Company Schedule for Heart Rhythm Society Scientific Sessions 3Boston Scientific Announces Company Schedule for Heart Rhythm Society Scientific Sessions 4Boston Scientific Announces Company Schedule for Heart Rhythm Society Scientific Sessions 5Pharmaceutical Packaging Equipment Manufacturer Boosts European Distribution 2Pharmaceutical Packaging Equipment Manufacturer Boosts European Distribution 3Baseline Research Indicates High Interest in Centocor-Ortho Biotech's SIMPONI(R) 2
... and efficient method for purification of DNA fragments ... fragments selectively absorbed in the spin column after ... room temperature for PCR product with equal volume ... DNA can be purified with a single prep ...
When added to either StemSep CD4+ or CD8+ T Cell Enrichment cocktails, human HLA-DR TAC is designed to deplete human HLA-DR from samples to isolate resting CD4+ or CD8+ T cells....
Fujifilm BAS-1800II provides the ideal configuration for a low-cost imager that requires only a small amount of space in the laboratory. The small imager for accurate high-throughput screening...
Request Info...
Biology Products: