Navigation Links
Researchers develop assay that could be applied to drug screening

Using state of the art imaging technology a team from Yale School of Medicine has glimpsed one of the cell's most important 'nano-machines' in action. The work, performed in collaboration with English and French scientists, provides new insight into the machinery cells use to internalize cell surface receptors.

All cells traffic protein cargos across their outer membrane, and one of the most important routes for cargo internalization is clathrin mediated endocytosis (CME). CME is of fundamental importance for many cellular activities including receptor down-regulation, nutrient uptake and maintenance of signal transmission across nerve cell junctions. Mis-regulation of CME has been implicated in some types of cancer and neuro-degenerative disease and the protein machinery of CME has been co-opted by several viruses, including rabies, as a means of entry into healthy cells.

The researchers led by David Zenisek, assistant professor in the Department of Cellular and Molecular Physiology at Yale School of Medicine, used live cell imaging and a novel fluorescence assay to visualize the formation of clathrin-coated vesicles (CCVs) at single clathrin-coated pits (CCPs) with a time resolution of seconds.

"Although the basic model of clathrin-mediated endocytosis was proposed 41years ago, there are many basic questions outstanding," Zenisek said. "For instance, it wasn't known whether single clathrin coated pits give rise to single clathrin coated vesicles. We have now shown directly that coated pits can produce multiple vesicles in succession."

Using a specially adapted microscope, Zenisek and his colleagues Christien Merrifield and David Perrais, simultaneously measured the minute movements made by coated pits as they invaginated and detected membrane scission, the process by which a coated pit is converted into a clathrin coated vesicle. In further experiments, the researchers showed how proteins linked to the actin framework of the cell are
'"/>

Source:Yale University


Page: 1 2

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Researchers develop assay that could applied drug screening

(Date:7/13/2015)... Conn. , Jul. 13, 2015 NXT-ID, ... "Company"), a biometric authentication company focused on the ... has filed provisional patent 62/188684 for ... achieves another new convenient and secure method to ... new, innovative payment methods, introduced with its groundbreaking ...
(Date:7/9/2015)... SAN JOSE, Calif. , July 9, ... ), the leading developer of human interface ... authentication technology, the industry,s first fully hardware ... innovative Match-in-Sensor secure authentication technology is literally ... pattern storage and biometric matching within the ...
(Date:7/8/2015)... 8, 2015 The consumer products industry ... must have products such as wearables, smart wallets & other ... biometrics based devices continues to rapidly grow.  Biometrics & Wearable ... NXTD ), Google Inc. (NASDAQ: GOOG ), Apple Inc. ... GoPro, Inc. (NASDAQ: GPRO ) and Baidu Inc. (NASDAQ: ...
Breaking Biology News(10 mins):NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 2Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 2Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 3Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 4Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 5Consumers Flock to This Summer's Must Have Tech Gadgets as Latest Digital Wallets, Smart Technology & Wearable Products Continue to Grow in Popularity & Functionality 6
... particles one-billionth of a meter in size can be ... brain cancer. What's more, these nanoparticles can be used ... them, according to researchers at the University of Michigan ... Photofrin along with iron oxide into nanoparticles that would ...
... soul," corneas are the panes in those windows. They shield ... the eye's outermost lens, contributing up to 75 percent of ... at the annual meeting of the American Chemical Society, chemical ... that's finding its way into artificial corneas. It's a hydrogel, ...
... in monoclonal antibody research for oncology offers a ... of antibody therapies against solid tumors. Writing in ... http://www.interscience.wiley.com/cancer-newsroom ), a peer-reviewed journal of the American ... of cancers involve solid tumors, but only 25 ...
Cached Biology News:U-M researchers use nanoparticles to target brain cancer 2U-M researchers use nanoparticles to target brain cancer 3An artificial cornea is in sight, thanks to biomimetic hydrogels 2An artificial cornea is in sight, thanks to biomimetic hydrogels 3An artificial cornea is in sight, thanks to biomimetic hydrogels 4Report calls for improved monoclonal antibodies against solid tumors 2
(Date:8/4/2015)... SAN CARLOS, Calif. , Aug. 4, 2015 /PRNewswire/ ... non-invasive genetic testing and the analysis of circulating cell-free ... its second quarter ended June 30, 2015 after the ... a conference call and webcast at 1:30 p.m. PT ... activities and financial outlook.Event:Natera,s Second Quarter 2015 Results Conference ...
(Date:8/3/2015)... -- Halozyme Therapeutics, Inc. (NASDAQ: HALO ) will webcast ... 2015 on Monday, August 10, 2015 at 4:30 p.m. ... and chief executive officer, will lead the call. On ... for the second quarter 2015. The call ... Halozyme,s corporate website and a recording will be made ...
(Date:8/3/2015)... UK (PRWEB) , ... August 03, 2015 , ... ... consumer of benzene. Benzene is processed to get such derivatives as ethylbenzene, a ... derivatives are principally used within the automotive industry for the production of tyres ...
(Date:8/3/2015)... , August 3, 2015 E-QURE ... commercialization of its Bio-electrical Signal Therapy device ("BST Device"), ... of hard to heal chronic wounds, today announced that ... Chemipal, an Israeli distributor specializing in medical devices, for ... Chemipal is a 70 years old, sales, marketing ...
Breaking Biology Technology:Natera to Release Second Quarter 2015 Results on August 12, 2015 2Halozyme to Host Second Quarter 2015 Financial Results Conference Call 2World Benzene Market to Surpass 46 MMT by 2020, According to TechSci Research Report Published at MarketPublishers.com 2World Benzene Market to Surpass 46 MMT by 2020, According to TechSci Research Report Published at MarketPublishers.com 3E-QURE Corp Signs Second Distribution Agreement in Israel 2E-QURE Corp Signs Second Distribution Agreement in Israel 3
... 2 Associated Cardiovascular Associates,(ACC), a 38-doctor ... in,Southern New Jersey, today announced that it ... and Sage Intergy practice management solution. The,Cherry ... than 30,000 patients,annually, will deploy the single-database, ...
... Track to File NDA with FDA in Early to ... LAKE, N.J., Dec. 2 Strativa Pharmaceuticals, the proprietary ... PRX ) today announced that its development partner, ... bioequivalence studies for a thin film formulation of ondansetron. ...
... InSightec Ltd. announced,today that new publications show ... system has the potential to be an,effective noninvasive ... bones (bone metastases). The results of a 31 ... of Annals of Surgical Oncology, while data from ...
Cached Biology Technology:Associated Cardiovascular Consultants Selects Sage Intergy EHR to Enhance Quality, Clinical Trial Research and Physician-Patient Relationship 2Associated Cardiovascular Consultants Selects Sage Intergy EHR to Enhance Quality, Clinical Trial Research and Physician-Patient Relationship 3Associated Cardiovascular Consultants Selects Sage Intergy EHR to Enhance Quality, Clinical Trial Research and Physician-Patient Relationship 4Strativa Pharmaceuticals Announces Successful Completion of Bioequivalence Studies for Ondansetron Thin Film 2Strativa Pharmaceuticals Announces Successful Completion of Bioequivalence Studies for Ondansetron Thin Film 3Strativa Pharmaceuticals Announces Successful Completion of Bioequivalence Studies for Ondansetron Thin Film 4New Publications Show Noninvasive Magnetic Resonance-Guided Focused Ultrasound to be a Potentially Safe, Effective Pain-Relieving Treatment for Bone Metastases 2New Publications Show Noninvasive Magnetic Resonance-Guided Focused Ultrasound to be a Potentially Safe, Effective Pain-Relieving Treatment for Bone Metastases 3New Publications Show Noninvasive Magnetic Resonance-Guided Focused Ultrasound to be a Potentially Safe, Effective Pain-Relieving Treatment for Bone Metastases 4
... Coated CELlulose) was developed in the late ... one of the earliest commercial pioneers of ... product range in 1987. Sac-Cel rapidly found ... their own "in house" radioimmunoassays, and became ...
... Our Accumix standard is a new ... mode electrospray ionization mass spectrometry. Accumix ... broad mass range coverage for both tuning ... LCQ- and LTQ-MS) and Fourier transform mass ...
Determin interaction of your protein with Echelons lipids...
... N-Dodecyl-N,N-(dimethylammonio)butyrate White ... properties similar to DDMAU (Cat. No. 252005) ... polymethylene arm. More efficient for the extraction ... than DDMAU. Purity: ≥98% by TLC. ...
Biology Products: