Navigation Links
Researchers develop assay that could be applied to drug screening

Using state of the art imaging technology a team from Yale School of Medicine has glimpsed one of the cell's most important 'nano-machines' in action. The work, performed in collaboration with English and French scientists, provides new insight into the machinery cells use to internalize cell surface receptors.

All cells traffic protein cargos across their outer membrane, and one of the most important routes for cargo internalization is clathrin mediated endocytosis (CME). CME is of fundamental importance for many cellular activities including receptor down-regulation, nutrient uptake and maintenance of signal transmission across nerve cell junctions. Mis-regulation of CME has been implicated in some types of cancer and neuro-degenerative disease and the protein machinery of CME has been co-opted by several viruses, including rabies, as a means of entry into healthy cells.

The researchers led by David Zenisek, assistant professor in the Department of Cellular and Molecular Physiology at Yale School of Medicine, used live cell imaging and a novel fluorescence assay to visualize the formation of clathrin-coated vesicles (CCVs) at single clathrin-coated pits (CCPs) with a time resolution of seconds.

"Although the basic model of clathrin-mediated endocytosis was proposed 41years ago, there are many basic questions outstanding," Zenisek said. "For instance, it wasn't known whether single clathrin coated pits give rise to single clathrin coated vesicles. We have now shown directly that coated pits can produce multiple vesicles in succession."

Using a specially adapted microscope, Zenisek and his colleagues Christien Merrifield and David Perrais, simultaneously measured the minute movements made by coated pits as they invaginated and detected membrane scission, the process by which a coated pit is converted into a clathrin coated vesicle. In further experiments, the researchers showed how proteins linked to the actin framework of the cell are
'"/>

Source:Yale University


Page: 1 2

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Researchers develop assay that could applied drug screening

(Date:10/14/2014)... how to produce enough food to feed the world ... Organization of the United Nations predicts that food production ... feed a growing global population, and plants are one ... Plants—grains, cereals, fruits, vegetables, and more—feed humans directly ... tap into our knowledge of how plants work to ...
(Date:10/14/2014)... doses of fish oil supplements, rich in omega-3 fatty acids, ... heartbeat in which the heart can beat as fast as ... led by the Montreal Heart Institute were published in the ... October 7th. , For the trial, 337 patients with atrial ... 4 grams of fish oil a day or to placebo ...
(Date:10/14/2014)... WORCESTER, MA – A team of scientists led ... School (UMMS) and the University of Miami Miller ... likely a key genetic pathway underlying bipolar (manic ... better drugs for treating bipolar affective disorder, as ... , The new findings, published online this week ...
Breaking Biology News(10 mins):Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3Rare genetic disease protects against bipolar disorder 2Rare genetic disease protects against bipolar disorder 3
... The Nature Conservancy and Conservation International (CI) have joined ... in one of the largest debt-for-nature swaps in history. ... Ricas most critically threatened tropical forests, with the United ... Costa Rica spending that amount on tropical forest conservation ...
... of Warwick and Astra Zeneca have found a new way ... hydrogen atoms and thus spot unwanted polymorphs in pharmaceuticals. ... which an active drug can actually exist in more than ... to act in very different ways. Now researchers at the ...
... important regulator of blood glucose levels, now has a ... University Medical Center researchers, working with colleagues in Texas, ... systems, which are involved in drug addiction and many ... targets for treating drug abuse, the findings raise questions ...
Cached Biology News:Costa Rica, US announce historic debt-for-nature swap 2Costa Rica, US announce historic debt-for-nature swap 3NMR researchers unlock hydrogen's secrets to spot polymorphism in pharmaceuticals 2Insulin's brain impact links drugs and diabetes 2Insulin's brain impact links drugs and diabetes 3
(Date:10/19/2014)... 19, 2014 The Asia-Pacific Speech Analytics ... in Asia-Pacific with analysis and forecast of revenue. This ... 2014 to $208 million by 2019, at a Compound ... 2019. , Browse through the TOC of the Asia-Pacific ... the in-depth analysis provided. It also provides a glimpse ...
(Date:10/19/2014)... According to market research report “Outdoor Wi-Fi Market ... Global Advancements, Forecasts and Analysis (2013 - 2018)", ... with analysis and forecasting of the global revenues. ... and the restrains for this market with the ... Browse 65 market data tables and 69 figures ...
(Date:10/18/2014)... The Asian arthroscopic devices report defines and ... forecast of revenue. The arthroscopic devices market in Asia ... at a CAGR of 6.5% from 2013 to 2018. ... devices market, to get an idea of the in-depth ... segmentation of arthroscopic devices market in the same region, ...
(Date:10/18/2014)... The Biomass Power industry has ... to 2014. The industry's expansion has been propelled in ... encouraged the use of biomass power. Furthermore, the majority ... require local utilities to generate electricity from renewable power ... campaigning for green technology also influenced industry performance by ...
Breaking Biology Technology:The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 2Biomass Power in the US Industry Market Research Report from IBISWorld Has Been Updated 3
... Massachusetts Governor Mitt Romney has drawn a thoughtful ethical line ... stem cell research in his state and to oppose another. ... news coverage of his announcement, it could define a more ... , ,In fact, if Romney's position became law in Wisconsin ...
... visualize thought? , ,Well, by pressing the open tip of ... hair against the membrane of an individual brain cell, ... flowing through individual ion channels on a cell's membrane. This ... a peek at the electrical activity of individual cells. , ...
... the DEMO conference last week in Scottsdale. Many of the ... 15 years have been introduced at this confab, which celebrated ... more than 250 million readers, listeners and viewers worldwide. , ... we can look at the state of the industry," said ...
Cached Biology Technology:Massachusetts stem-cell plan reflects status quo for Wisconsin research 2Massachusetts stem-cell plan reflects status quo for Wisconsin research 3Prairie Technologies works with UW engineer to visualize the brain 2In search of the next big thing at DEMO 2In search of the next big thing at DEMO 3
MitoScreen (JC-1) Kit , 100 tests Consult technical datasheet for details....
Conjugate Quality Control Evaluation Specificity: Conjugate Quality Control Evaluation ...
... Production Service - Conjugation ... Biotin Conjugation Fluorescein Isothiocyanate Conjugation ... Allophycocyanin Conjugation Alexa Fluor 488 ... Alexa Fluor 405 Conjugation Rpe-Alexa Fluor ...
Request Info...
Biology Products: