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New law for resolution allows unprecedented sharpness in fluorescence microscopy

edients and protein interactions in cells in future.

Original work:

[1]Hell, S. W.
Strategy for far-field optical imaging and writing without diffraction limit.
Phys. Lett. A 326(1-2): 140-145 (2004)

[2] Westphal, V., and S. W. Hell
Nanoscale Resolution in the Focal Plane of an Optical Microscope.
Phys. Rev. Lett. 94: 143903 (2005)

[3] Westphal, V., J. Seeger, T. Salditt and S. W. Hell
Stimulated Emission Depletion Microscopy on Lithographic Nanostructures.
J. Phys. B: At. Mol. Opt. Phys. 38: S695 - S705 (2005)

[4] Kastrup, L., H. Blom, C. Eggeling, S. W. Hell
Fluorescence Fluctuation Spectroscopy in Subdiffraction Focal Volumes.
Phys. Rev. Lett. 94: 178104 (2005)


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Source:Max-Planck-Gesellschaft


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Biotin or fluorescent labels can be attached and the stoichiometry of protein labeling determined....
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